[en] Diagnosis of bovine tuberculosis in cattle is challenging due to complex immune host response to infection that limit the performance of available diagnostic tests. In this study, performance of two commercial serological assays developed to detect bovine tuberculosis were evaluated: Enferplex Bovine TB antibody kit including 11 antigens (EnferGroup, Ireland) and IDEXX M. bovis Ab kit (IDEXX, USA). The specificity value obtained with the ELISA IDEXX M. bovis Ab test was 97.1%, whereas it was 97.1% and 95.1% for the high specificity and sensitivity settings, respectively, with the Enferplex Bovine TB antibody kit. The sensitivity of the multiplexed Enferplex Bovine TB antibody test for SICCT-positive animals was higher (N = 172; 51.7% and 58.7% with high specificity and sensitivity settings, respectively) compared to the ELISA IDEXX M. bovis Ab test (sensitivity of 36.6%). "Antigen profiles" generated by the multiplexed Enferplex method showed that five out of 11 antigens present in the test were mostly identified as positive sera in cattle originating from bTB-outbreaks. In comparison, unique profiles appeared to be correlated with false positive results. However additional studies are needed to confirm the observed antigen profiles, and their potential use as an additional diagnostic tool. Serial interpretation of the two serological tests produced higher diagnostic specificity (>99%), reducing false positive results, which is essential for a screening test when the prevalence of bovine tuberculosis is low.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Moens, Charlotte; Veterinary Bacteriology Service, Sciensano, Brussels B-1050, Belgium, Louvain Institute of Biomolecular Science and Technology, Université Catholique de Louvain, Louvain-la-Neuve B-1348, Belgium
Saegerman, Claude ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Epidémiologie et analyse des risques appliqués aux sciences vétérinaires
FOD Volksgezondheid - Federale Overheidsdienst Volksgezondheid - Veiligheid van de Voetselketen en Leefmilieu [BE]
Funding text :
The research that yielded these results, was funded by the Belgian Federal Public Service of Health, Food Chain Safety and Environment through the contract RT 18/01 DIBOTUB. Preliminary results were presented as a poster at the Seventh International Conference on Mycobacterium bovis, Galway, 7-10 June 2022. We thank members from “Association Régionale de Santé et d'Identification Animales” (ARSIA) for providing the serum samples used in this study. We also thank Ana Soares, Philippe Vannoorenberghe, Damien Desqueper, and Willem Van Campe for providing technical assistance.The research that yielded these results, was funded by the Belgian Federal Public Service of Health , Food Chain Safety and Environment through the contract RT 18/01 DIBOTUB . Preliminary results were presented as a poster at the Seventh International Conference on Mycobacterium bovis, Galway, 7-10 June 2022. We thank members from “Association Régionale de Santé et d'Identification Animales” (ARSIA) for providing the serum samples used in this study. We also thank Ana Soares, Philippe Vannoorenberghe, Damien Desqueper, and Willem Van Campe for providing technical assistance.
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