[en] Phosphorus deficiency induces biochemical and morphological changes which affect crop yield and production. Prompt fluorescence signal characterizes the PSII activity and electron transport from PSII to PSI, while the modulated light reflection at 820 (MR 820) nm investigates the redox state of photosystem I (PSI) and plastocyanin (PC). Therefore, combining information from modulated reflection at 820 nm with chlorophyll a fluorescence can potentially provide a more complete understanding of the photosynthetic process and integrating other plant physiological measurements may help to increase the accuracy of detecting the phosphorus deficiency in wheat leaves. In our study, we combined the chlorophyll a fluorescence and MR 820 signals to study the response of wheat plants to phosphorus deficiency as indirect tools for phosphorus plant status characterization. In addition, we studied the changes in chlorophyll content index, stomatal conductance (gs), root morphology, and biomass of wheat plants. The results showed an alteration in the electron transport chain as a specific response to P deficiency in the I-P phase during the reduction of the acceptor side of PSI. Furthermore, P deficiency increased parameters related to the energy fluxes per reaction centers, namely ETo/RC, REo/RC, ABS/RC, and DIo/RC. P deficiency increased the values of MRmin and MRmax and decreased νred, which implies that the reduction of PSI and PC became slower as the phosphorus decreased. The principal component analysis of the modulated reflection and chlorophyll a fluorescence parameters, with the integration of the growth parameters as supplementary variables, accounted for over 71% of the total variance in our phosphorus data using two components and provided a reliable information on PSII and PSI photochemistry under P deficiency.
El-Mejjaouy, Yousra ; Université de Liège - ULiège > TERRA Research Centre ; AgoBioSciences, Plant Stress Physiology Laboratory, University Mohammed VI Polytechnic (UM6P), Benguerir, Morocco
Belmrhar, Laila; AgoBioSciences, Plant Stress Physiology Laboratory, University Mohammed VI Polytechnic (UM6P), Benguerir, Morocco
Zeroual, Youssef; AgoBioSciences, Plant Stress Physiology Laboratory, University Mohammed VI Polytechnic (UM6P), Benguerir, Morocco
Dumont, Benjamin ; Université de Liège - ULiège > TERRA Research Centre > Plant Sciences
Mercatoris, Benoît ; Université de Liège - ULiège > TERRA Research Centre > Biosystems Dynamics and Exchanges (BIODYNE)
Oukarroum, Abdallah ; AgoBioSciences, Plant Stress Physiology Laboratory, University Mohammed VI Polytechnic (UM6P), Benguerir, Morocco ; High Throughput Multidisciplinary Research Laboratory, University Mohammed VI Polytechnic (UM6P), Benguerir, Morocco
Language :
English
Title :
PCA-based detection of phosphorous deficiency in wheat plants using prompt fluorescence and 820 nm modulated reflection signals.
OCP - OCP Group SA Prayon UM6P ULiège - Université de Liège
Funding text :
This work was supported by the Funders of the SoilPhorLife Program, OCP Group (https:// www.ocpgroup.ma/) and Prayon (https://www. prayon.com/en/company/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. For the chemical analysis of soil and plant samples, the authors acknowledge the assistance of Mr Aziz Soulaimani and Mrs Sabah Fathallah.
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