Reference : Steady-state levels of imported tRNAs in Chlamydomonas mitochondria are correlated wi...
Scientific journals : Article
Life sciences : Genetics & genetic processes
Steady-state levels of imported tRNAs in Chlamydomonas mitochondria are correlated with both cytosolic and mitochondrial codon usages
Vinogradova, Elizaveta [> > > >]
Salinas, Thalia [> > > >]
Cognat, Valerie [> > > >]
Remacle, Claire mailto [Université de Liège - ULiège > Département des sciences de la vie > Génétique >]
Marechal-Drouard, Laurence [> > > >]
Nucleic Acids Research
Oxford University Press
Yes (verified by ORBi)
United Kingdom
[en] The mitochondrial genome of Chlamydomonas reinhardtii only encodes three expressed tRNA genes, thus most mitochondrial tRNAs are likely imported. The sharing of tRNAs between chloroplasts and mitochondria has been speculated in this organism. We first demonstrate that no plastidial tRNA is present in mitochondria and that the mitochondrial translation mainly relies on the import of nucleus-encoded tRNA species. Then, using northern analysis, we show that the extent of mitochondrial localization for the 49 tRNA isoacceptor families encoded by the C. reinhardtii nuclear genome is highly variable. Until now the reasons for such variability were unknown. By comparing cytosolic and mitochondrial codon usage with the sub-cellular distribution of tRNAs, we provide unprecedented evidence that the steady-state level of a mitochondrial tRNA is linked not only to the frequency of the cognate codon in mitochondria but also to its frequency in the cytosol, then allowing optimal mitochondrial translation.
C Remacle and L Marechal Drouard are co-corresponding authors

File(s) associated to this reference

Fulltext file(s):

Restricted access
Vinogradova et al 2009.pdfPublisher postprint499.46 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.