micropillar array column; multiplexing; proteomics; Ion Mobility Spectrometry; Mass Spectrometry
Abstract :
[en] Proteomics is one of the most significant methodologies to better understand the molecular pathways involved in diseases and to improve their diagnosis, treatment and follow-up. The investigation of the proteome of complex organisms is challenging from an analytical point of view, because of the large number of proteins present in a wide range of concentrations. In this study, nanofluidic chromatography, using a micropillar array column, was coupled to drift-tube ion mobility and time-of-flight mass spectrometry to identify as many proteins as possible in a protein digest standard of HeLa cells. Several chromatographic parameters were optimized. The high interest of drift-tube ion mobility to increase the number of identifications and to separate isobaric coeluting peptides was demonstrated. Multiplexed drift-tube ion mobility spectrometry was also investigated, to increase the sensitivity in proteomics studies. This innovative proteomics platform will be useful for analyzing patient samples to better understand unresolved disorders.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Nix, Cindy ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Cobraiville, Gaël ; Centre Hospitalier Universitaire de Liège - CHU > > Service de rhumatologie
Gou, Marie-Jia ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Fillet, Marianne ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Language :
English
Title :
Potential of Single Pulse and Multiplexed Drift-Tube Ion Mobility Spectrometry Coupled to Micropillar Array Column for Proteomics Studies.
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Funding text :
Funding: This research was funded by the Belgian National Fund for Scientific Research (F. N.R. S.), grant number: 40001038 and the Fondation Léon Frédéricq. The authors acknowledge the research grants from the Walloon Region of Belgium and EU Commission (project FEDER-PHARE) for the funding of the DTIMS–QTOF instrument.
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