[en] The vertebrate piggyBac derived transposase 5 (PGBD5) encodes a domesticated transposase, which is active and able to transpose its distantly related piggyBac-like element (pble), Ifp2. This raised the question whether PGBD5 would be more effective at mobilizing a phylogenetically closely related pble element. We aimed to identify the pble most closely related to the pgbd5 gene. We updated the landscape of vertebrate pgbd genes to develop efficient filters and identify the most closely related pble to each of these genes. We found that Tcr-pble is phylogenetically the closest pble to the pgbd5 gene. Furthermore, we evaluated the capacity of two murine and human PGBD5 isoforms, Mm523 and Hs524, to transpose both Tcr-pble and Ifp2 elements. We found that both pbles could be transposed by Mm523 with similar efficiency. However, integrations of both pbles occurred through both proper transposition and improper PGBD5-dependent recombination. This suggested that the ability of PGBD5 to bind both pbles may not be based on the primary sequence of element ends, but may involve recognition of inner DNA motifs, possibly related to palindromic repeats. In agreement with this hypothesis, we identified internal palindromic repeats near the end of 24 pble sequences, which display distinct sequences.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Helou, Laura ; Université de Liège - ULiège > Département des sciences cliniques ; UMR INRAE 0085, CNRS 7247, Physiologie de la Reproduction et des Comportements, Centre INRA Val de Loire, 37380 Nouzilly, France
Beauclair, Linda; UMR INRAE 0085, CNRS 7247, Physiologie de la Reproduction et des Comportements, Centre INRA Val de Loire, 37380 Nouzilly, France
Dardente, Hugues; UMR INRAE 0085, CNRS 7247, Physiologie de la Reproduction et des Comportements, Centre INRA Val de Loire, 37380 Nouzilly, France
Piégu, Benoît; UMR INRAE 0085, CNRS 7247, Physiologie de la Reproduction et des Comportements, Centre INRA Val de Loire, 37380 Nouzilly, France
Tsakou-Ngouafo, Louis; UMR MEPHI D-258, I, IRD, Aix Marseille Université, 19-21 Boulevard Jean Moulin, 13005 Marseille, France, CNRS SNC 5039, 13005 Marseille, France
Lecomte, Thierry; EA GICC 7501, CHRU de Tours, 37044 TOURS Cedex 09, France
Kentsis, Alex; Molecular Pharmacology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, USA, Weill Cornell Medical College, Cornell University, New York, NY, USA, Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, NY, USA
Pontarotti, Pierre; UMR INRAE 0085, CNRS 7247, Physiologie de la Reproduction et des Comportements, Centre INRA Val de Loire, 37380 Nouzilly, France, CNRS SNC 5039, 13005 Marseille, France
Bigot, Yves; UMR INRAE 0085, CNRS 7247, Physiologie de la Reproduction et des Comportements, Centre INRA Val de Loire, 37380 Nouzilly, France. Electronic address: yves.bigot@inrae.fr
Language :
English
Title :
The piggyBac-derived protein 5 (PGBD5) transposes both the closely and the distantly related piggyBac-like elements Tcr-pble and Ifp2.
SNFGE - Société Nationale Française de Gastro-Entérologie Merck Ligue Nationale Contre le Cancer Region Centre-Val de Loire
Funding text :
This work was supported by the C.N.R.S., the I.N.R.A., and the GDR CNRS 2157. It also received funds from a research program grants from the Ligue Nationale Contre le Cancer, the Merck foundation, and the French National Society of Gastroenterology. Laura Helou holds a PhD fellowship from the Région Centre Val de Loire. We acknowledge the high-throughput sequencing facilities of I2BC for its sequencing and bioinformatics expertize. Alex Kentsis is a consultant for Novartis and is supported by the National Cancer Institute grants R01 CA214812 and P30 CA008748. Yves Bigot, who was in charge of the achievement of this project does not have to thank the French National Research Agency for its financial support but he kindly thanks it for the excellent reviews embellished with arguments based on scientific and cultural novelties in the expertise of his yearly application file during the last decade. Our thanks to Peter Arensburger at California Polytechnic University in Pomona CA (Cal Poly Pomona) for reviewing the manuscript for legibility.This work was supported by the C.N.R.S. the I.N.R.A. and the GDR CNRS 2157. It also received funds from a research program grants from the Ligue Nationale Contre le Cancer, the Merck foundation, and the French National Society of Gastroenterology. Laura Helou holds a PhD fellowship from the R?gion Centre Val de Loire. We acknowledge the high-throughput sequencing facilities of I2BC for its sequencing and bioinformatics expertize. Alex Kentsis is a consultant for Novartis and is supported by the National Cancer Institute grants R01 CA214812 and P30 CA008748. Yves Bigot, who was in charge of the achievement of this project does not have to thank the French National Research Agency for its financial support but he kindly thanks it for the excellent reviews embellished with arguments based on scientific and cultural novelties in the expertise of his yearly application file during the last decade. Our thanks to Peter Arensburger at California Polytechnic University in Pomona CA (Cal Poly Pomona) for reviewing the manuscript for legibility. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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