Biomonitoring; Birds; Blood; Pesticides; Plasma; moexipril; Pesticide Residues; Animals; Tandem Mass Spectrometry/methods; Chromatography, Liquid/methods; Pesticides/analysis; Galliformes; Pesticide Residues/analysis; Analytical method; Blood samples; GC/MS/MS; LC-MS-MS; Pesticide contaminations; Plant protection products; Plasma samples; Wild birds; Chromatography, Liquid; Tandem Mass Spectrometry; Analytical Chemistry; Biochemistry; Organic Chemistry; General Medicine
Abstract :
[en] In this study, blood and plasma of grey partridges (Perdix perdix) were analyzed to assess their potential contamination by plant protection products (PPP) and especially pesticide compounds. The group of pesticides selected is composed of a huge variety of compounds. Therefore, in this study, two methods were optimized and validated to analyze 104 compounds including herbicides, insecticides, fungicides and photoprotectors or synergists. Various extraction methods found in the literature were compared and adapted for the extraction of pesticides from blood and plasma. After extraction, samples were concentrated then injected for quantification simultaneously in LC-MS/MS and ATD-GC-MS/MS with an automatic thermal desorption step (ATD). Both LC-MS/MS and ATD-GC-MS/MS analyses were performed using the MRM mode with 2 mass transitions for each compound.The two analytical methods achieved a good linearity for the calibration responses in plasma and blood. Methods allowed sensitive detection and quantification in complex biological matrices such as plasma and blood in both LC and GC. For plasma samples and considering all 104 compounds of the study, the average LOD was 0.005 ng mg-1 in LC-MS/MS and 0.035 ng mg-1 in ATD-GC-MS/MS and the average LOQ was 0.017 ng mg-1 and 0.116 ng mg-1 in LC-MS/MS and ATD-GC-MS/MS respectively. Accordingly, the average LOD for blood samples was 0.011 ng mg-1 in LC and 0.028 ng mg-1 in GC whereas the average LOQ was 0.038 ng mg-1 and 0.094 ng mg-1 in LC-MS/MS and ATD-GC-MS/MS respectively. Those analytical methods were then successfully applied to 70 blood samples and 35 plasma samples.
Disciplines :
Chemistry
Author, co-author :
Rodrigues, Anaïs ; Université de Liège - ULiège > Molecular Systems (MolSys) ; Université de Strasbourg, CNRS-UMR 7515, ICPEES, 67087 Strasbourg cedex 2, France. Electronic address: anaisrodrigues@unistra.fr
Gaffard, A ; Centre d'Études Biologiques de Chizé, UMR 7372, CNRS & La Rochelle Université, 79360 Villiers-en-Bois, France
Moreau, J; Centre d'Études Biologiques de Chizé, UMR 7372, CNRS & La Rochelle Université, 79360 Villiers-en-Bois, France, UMR CNRS 6282 Biogéosciences, Université Bourgogne Franche-Comté, 6 Boulevard Gabriel, 21000 Dijon, France
Monceau, K ; Centre d'Études Biologiques de Chizé, UMR 7372, CNRS & La Rochelle Université, 79360 Villiers-en-Bois, France
Delhomme, O; Université de Strasbourg, CNRS-UMR 7515, ICPEES, 67087 Strasbourg cedex 2, France, UFR Sciences fondamentales et appliquées, Université de Lorraine, Campus Bridoux, 57070 Metz, France
Millet, M; Université de Strasbourg, CNRS-UMR 7515, ICPEES, 67087 Strasbourg cedex 2, France
Language :
English
Title :
Analytical development for the assessment of pesticide contaminations in blood and plasma of wild birds: The case of grey partridges (Perdix perdix).
This work was supported by the University of La Rochelle, the French National Centre of Scientific Research (CNRS), and the French National Research Institute for Agriculture, Food and the Environment (INRAE). This study was partly funded by ANR JCJC PestiStress (#19-CE34-0003-01), the BioBird project funded by the regional government of Nouvelle-Aquitaine, the project BIRDPEST funded by RECOTOX 2019, ACI funded by La Rochelle Université, AgriBioBird funded by OSU Theta ISITE-BFC, the French National program EC2CO (Ecosphère Continentale et Côtière), and the Contrat de Plan Etat-Région (CPER) Econat.
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