Endolymph; Organotypic culture; Potassium; Rat; Styrene; Vestibular explants; Animals; Animals, Newborn; Endolymph/drug effects; Endolymph/metabolism; Female; Potassium/metabolism; Rats, Long-Evans; Saccule and Utricle/drug effects; Saccule and Utricle/metabolism; Saccule and Utricle/pathology; Styrene/toxicity; Saccule and Utricle; Toxicology; General Medicine
Abstract :
[en] Despite well-documented neurotoxic and ototoxic properties, styrene remains commonly used in industry. Its effects on the cochlea have been extensively studied in animals, and epidemiological and animal evidence indicates an impact on balance. However, its influence on the peripheral vestibular receptor has yet to be investigated. Here, we assessed the vestibulotoxicity of styrene using an in vitro model, consisting of three-dimensional cultured newborn rat utricles filled with a high‑potassium (K+) endolymph-like fluid, called "cysts". K+ entry in the cyst ("influx") and its exit ("efflux") are controlled by secretory cells and hair cells, respectively. The vestibular epithelium's functionality is thus linked to K+ concentration, measured using a microelectrode. Known inhibitors of K+ efflux and influx validated the model. Cysts were subsequently exposed to styrene (0.25; 0.5; 0.75 and 1 mM) for 2 h or 72 h. The decrease in K+ concentration measured after both exposure durations was dose-dependent, and significant from 0.75 mM styrene. Vacuoles were visible in the cytoplasm of epithelial cells from 0.5 mM after 2 h and from 0.25 mM after 72 h. The results presented here are the first evidence that styrene may deregulate K+ homeostasis in the endolymphatic space, thereby altering the functionality of the vestibular receptor.
Tallandier, Valentin ; Université de Liège - ULiège > GIGA > GIGA Neurosciences ; Institut National de Recherche et de Sécurité, Rue du Morvan, CS 60027, F-54519 Vandœuvre, Cedex, France, DevAH EA 3450 - Développement, Adaptation et Handicap, Régulations cardio-respiratoires et de la motricité-Université de Lorraine, F-54500 Vandœuvre, France
Merlen, L; Institut National de Recherche et de Sécurité, Rue du Morvan, CS 60027, F-54519 Vandœuvre, Cedex, France
Boucard, S; Institut National de Recherche et de Sécurité, Rue du Morvan, CS 60027, F-54519 Vandœuvre, Cedex, France
Thomas, A; Institut National de Recherche et de Sécurité, Rue du Morvan, CS 60027, F-54519 Vandœuvre, Cedex, France
Venet, T; Institut National de Recherche et de Sécurité, Rue du Morvan, CS 60027, F-54519 Vandœuvre, Cedex, France, DevAH EA 3450 - Développement, Adaptation et Handicap, Régulations cardio-respiratoires et de la motricité-Université de Lorraine, F-54500 Vandœuvre, France
Chalansonnet, M; Institut National de Recherche et de Sécurité, Rue du Morvan, CS 60027, F-54519 Vandœuvre, Cedex, France. Electronic address: monique.chalansonnet@inrs.fr
Gauchard, G; DevAH EA 3450 - Développement, Adaptation et Handicap, Régulations cardio-respiratoires et de la motricité-Université de Lorraine, F-54500 Vandœuvre, France
Campo, P; Institut National de Recherche et de Sécurité, Rue du Morvan, CS 60027, F-54519 Vandœuvre, Cedex, France, DevAH EA 3450 - Développement, Adaptation et Handicap, Régulations cardio-respiratoires et de la motricité-Université de Lorraine, F-54500 Vandœuvre, France
Pouyatos, B; Institut National de Recherche et de Sécurité, Rue du Morvan, CS 60027, F-54519 Vandœuvre, Cedex, France
Language :
English
Title :
Styrene alters potassium endolymphatic concentration in a model of cultured utricle explants.
Publication date :
September 2020
Journal title :
Toxicology in Vitro: an International Journal Published in Association with BIBRA
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