Poster (Scientific congresses and symposiums)
Evaluation of a commercial IgG monotest assay: a new automated chemiluminescent immunoassay for the serodiagnosis of cystic echinococcosis
Egrek, Sabrina; Sacheli, Rosalie; Beaumaikers, Elise et al.
202331rd European Congress of Clinical Microbiology & Infectious Diseases (ECCMID)
 

Files


Full Text
ECCMID 2023_ SabrinaEgrek_P3098.pdf
Author postprint (1.67 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Cystic echinococcosis; diagnosis; serology; immunoassay; National Reference Laboratory for Echinococcosis
Abstract :
[en] Background: Cystic echinococcosis (CE) is a zoonotic disease caused by the tapeworm Echinococcus granulosus complex. The geographical distribution is worldwide with variable incidences. In Belgium, only few imported cases are reported each year. Serodiagnosis of CE is performed by using a combination of immunoassays which are mainly based on crude hydatid antigens. The Belgian National Reference Laboratory, has evaluated the CE-IVD Hydatidosis VirClia® IgG chemiluminescent immunoassay and compared it with two other immunoassays. Methods: A total of 79 sera were retrospectively included from 15 patients with CE, 29 with alveolar echinococcosis, 16 with toxocariasis and 19 negative controls. Three immunoassays were compared: the Hydatidosis VirClia® IgG monotest assay which was run on the Virclia® Lotus (Vircell, Spain); the Ridascreen® Echinococcus IgG assay (R-Biopharm, Germany) and the Bordier® Echinococcus granulosus IgG ELISA (Bordier, Switzerland), which were tested on the ETI-Max 3000 immunoassay analyzer (DiaSorin, Italy). The McNemar test is used for statistical analysis. Results: All three methods showed 100% sensitivity. Regarding specificity, the Ridascreen® (78.1%) and VirClia® (76.6%) assays showed comparable performance (p-value: 1), while the Bordier® assay had poor results (54,7%) (p-value: 0,0007). The Bordier® assay showed 76% cross-reactions with E. multilocularis (22/29) and 31% with Toxocara sp. (5/16), while the VirClia® assay showed 51,7% (15/29) and no cross-reaction with Toxocara antigens. For Ridascreen® assay, 34% and 19% cross-reactions were observed for E. multilocularis (10/29) and Toxocara sp. (3/16), respectively. Non-specific reactions in negative controls were only observed with the Ridascreen® (1/19) and Bordier® assays (2/19). The shortest turnaround time was observed with Virclia® Lotus: 1 hour versus 3 hours for two other assays. Conclusions: All assays showed very high sensitivity. However, regarding specificity, the VirClia® performs better than the Bordier® and similarly to the Ridascreen® assay. Besides, the ready-to-use monotest format offers many advantages such as a quicker methodology and a reduced workflow. Therefore, the VirClia® assay is an efficient screening method for the detection of CE but should always be combined with an immunoblot assay to assess the specificity.
Disciplines :
Microbiology
Author, co-author :
Egrek, Sabrina ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de microbiologie clinique
Sacheli, Rosalie  ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de microbiologie clinique
Beaumaikers, Elise ;  Université de Liège - ULiège > Faculté de Médecine > Master sc. pharma., à fin.
Toussaint, Françoise;  CHU Liège - Central University Hospital of Liege [BE] > Service de Microbiologie Clinique, Sérologie infectieuse
Giot, Jean-Baptiste ;  Centre Hospitalier Universitaire de Liège - CHU > > Service des maladies infectieuses - médecine interne
Léonard, Philippe ;  Centre Hospitalier Universitaire de Liège - CHU > > Service des maladies infectieuses - médecine interne
Detry, Olivier  ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de chirurgie abdo, sénologique, endocrine et de transplantation
Truyens, Carine;  ULB - Université Libre de Bruxelles [BE] > Laboratoire de Parasitologie
Hayette, Marie-Pierre ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de microbiologie clinique
Language :
English
Title :
Evaluation of a commercial IgG monotest assay: a new automated chemiluminescent immunoassay for the serodiagnosis of cystic echinococcosis
Publication date :
17 April 2023
Event name :
31rd European Congress of Clinical Microbiology & Infectious Diseases (ECCMID)
Event date :
17 april 2023
By request :
Yes
Audience :
International
Available on ORBi :
since 16 May 2023

Statistics


Number of views
98 (9 by ULiège)
Number of downloads
27 (2 by ULiège)

Bibliography


Similar publications



Contact ORBi