Arabidopsis Proteins; Lipids; Sterols; Phytochrome; Carbon; Autophagy/genetics; Carbon/metabolism; Cell Membrane/metabolism; Gene Expression Regulation, Plant; Hypocotyl/genetics; Light; Sterols/metabolism; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Phytochrome/metabolism; Arabidopsis; Autophagy; Cell Membrane; Hypocotyl; Chemistry (all); Biochemistry, Genetics and Molecular Biology (all); Multidisciplinary; Physics and Astronomy (all); General Physics and Astronomy; General Biochemistry, Genetics and Molecular Biology; General Chemistry
Abstract :
[en] Plant growth ultimately depends on fixed carbon, thus the available light for photosynthesis. Due to canopy light absorption properties, vegetative shade combines low blue (LB) light and a low red to far-red ratio (LRFR). In shade-avoiding plants, these two conditions independently trigger growth adaptations to enhance light access. However, how these conditions, differing in light quality and quantity, similarly promote hypocotyl growth remains unknown. Using RNA sequencing we show that these two features of shade trigger different transcriptional reprogramming. LB induces starvation responses, suggesting a switch to a catabolic state. Accordingly, LB promotes autophagy. In contrast, LRFR induced anabolism including expression of sterol biosynthesis genes in hypocotyls in a manner dependent on PHYTOCHROME-INTERACTING FACTORs (PIFs). Genetic analyses show that the combination of sterol biosynthesis and autophagy is essential for hypocotyl growth promotion in vegetative shade. We propose that vegetative shade enhances hypocotyl growth by combining autophagy-mediated recycling and promotion of specific lipid biosynthetic processes.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Ince, Yetkin Çaka ; Center for Integrative Genomics, Faculty of Biology and Medicine, Génopode Building, University of Lausanne, CH-1015, Lausanne, Switzerland
Krahmer, Johanna; Center for Integrative Genomics, Faculty of Biology and Medicine, Génopode Building, University of Lausanne, CH-1015, Lausanne, Switzerland
Fiorucci, Anne-Sophie ; Center for Integrative Genomics, Faculty of Biology and Medicine, Génopode Building, University of Lausanne, CH-1015, Lausanne, Switzerland
Trevisan, Martine ; Center for Integrative Genomics, Faculty of Biology and Medicine, Génopode Building, University of Lausanne, CH-1015, Lausanne, Switzerland
Galvão, Vinicius Costa; Center for Integrative Genomics, Faculty of Biology and Medicine, Génopode Building, University of Lausanne, CH-1015, Lausanne, Switzerland
Wigger, Leonore ; Genomic Technologies Facility, Faculty of Biology and Medicine, Génopode Building, University of Lausanne, CH-1015, Lausanne, Switzerland
Pradervand, Sylvain; Genomic Technologies Facility, Faculty of Biology and Medicine, Génopode Building, University of Lausanne, CH-1015, Lausanne, Switzerland
Fouillen, Laetitia ; Univ. Bordeaux, CNRS, Laboratoire de Biogenèse Membranaire, UMR 5200, F-33140, Villenave d'Ornon, France
Van Delft, Pierre ; Univ. Bordeaux, CNRS, Laboratoire de Biogenèse Membranaire, UMR 5200, F-33140, Villenave d'Ornon, France
Genva, Manon ; Université de Liège - ULiège > Département GxABT > Chemistry for Sustainable Food and Environmental Systems (CSFES) ; Univ. Bordeaux, CNRS, Laboratoire de Biogenèse Membranaire, UMR 5200, F-33140, Villenave d'Ornon, France
Mongrand, Sebastien; Univ. Bordeaux, CNRS, Laboratoire de Biogenèse Membranaire, UMR 5200, F-33140, Villenave d'Ornon, France
Gallart-Ayala, Hector; Metabolomics Platform, Faculty of Biology and Medicine, Rue du Bugnon 19, University of Lausanne, CH-1005, Lausanne, Switzerland
Ivanisevic, Julijana ; Metabolomics Platform, Faculty of Biology and Medicine, Rue du Bugnon 19, University of Lausanne, CH-1005, Lausanne, Switzerland
Fankhauser, Christian ; Center for Integrative Genomics, Faculty of Biology and Medicine, Génopode Building, University of Lausanne, CH-1015, Lausanne, Switzerland. christian.fankhauser@unil.ch
F.R.S.-FNRS - Fonds de la Recherche Scientifique ANR - Agence Nationale de la Recherche SNF - Schweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung ULiège - Université de Liège UNIL - Université de Lausanne
Work in the Fankhauser lab is supported by the University of Lausanne and the Swiss National Science Foundation (310030B_179558) and the Velux Foundation (Project 1455). The Bordeaux Metabolome-Lipidome Facility-MetaboHUB by a grant from ANR (no. ANR–11–INBS–0010). Manon Genva thanks the National Fund for Scientific Research (FNRS, Belgium, through grant 2022/V 3/5/053-40010130-JG/JN-2724) and the University of Liège for mobility grants.We thank Martina Legris, Laure Allenbach Petrolati, Olivier Michaud, Mieke de Wit, Anupama Goyal, Ana Lopez Vazquez, Ganesh Mahadeo Nawkar, and Maud Lagier for providing resources, technical support and/or comments on the manuscript; René Dreos for advice on statistical methods; Niko Geldner, Ersin Gül (ETH Zurich), Sinem Celebioven (University Zurich), Soner Yildiz (Icahn School of Medicine) and Yasin Dagdas (GMI, Vienna) for suggestions and comments on the manuscript; Christian Hardtke, Teva Vernoux (ENS Lyon), Richard Vierstra (Washington University, St Louis), Dany Geelen (University Gent) and Yasin Dagdas for providing resources. We are grateful to the CIF ( https://cif.unil.ch/ ) for help with microscopy, the GTF ( https://wp.unil.ch/gtf/ ) for RNA-seq. experiments and the Metabolomics Unit ( https://www.unil.ch/metabolomics/en/home.html ) for the lipidomic analysis and the Bordeaux-Metabolome platform for sterol analysis ( https://www.biomemb.cnrs.fr/en/lipidomic-plateform/ ). Work in the Fankhauser lab is supported by the University of Lausanne and the Swiss National Science Foundation (310030B_179558) and the Velux Foundation (Project 1455). The Bordeaux Metabolome-Lipidome Facility-MetaboHUB by a grant from ANR (no. ANR–11–INBS–0010). Manon Genva thanks the National Fund for Scientific Research (FNRS, Belgium, through grant 2022/V 3/5/053-40010130-JG/JN-2724) and the University of Liège for mobility grants.
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