[en] Zinc finger homeodomain (ZF-HD) transcription factors play significant roles in plant growth and responses to environmental stresses. In this study, 32 ZF-HD genes identified in the tobacco (Nicotiana tabacum L.) genome were divided into six groups according to phylogenetic analysis with Arabidopsis and tomato ZF-HD members. An examination of gene structures and conserved motifs revealed the relatively conserved exon/intron structures and motif organization within each subgroup. In addition, various stress-related elements are found in the promoter region of these genes. The expression profiling analysis revealed that NtZF-HD genes expressed in different tissues and could be induced by several abiotic stresses. Notably, NtZF-HD21 was highly expressed in response to the drought treatments. Subcellular localization analysis and a virus-induced gene silencing (VIGS) experiment were performed to investigate the potential functions of NtZF-HD21. The subcellular localization indicated that NtZF-HD21 is a nuclear protein. Furthermore, gene silencing of the NtZF-HD21 gene reduced the drought resistance of tobacco. These findings provide insights for further biological functional analyses of the NtZF-HD genes in tobacco.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Sun, Jinhao ; Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China ; Graduate School of Chinese Academy of Agricultural Science, Beijing, China
Xie, Minmin ; Université de Liège - ULiège > TERRA Research Centre ; Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China ; Graduate School of Chinese Academy of Agricultural Science, Beijing, China
Li, Xiaoxu ; Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China ; Technology Center, China Tobacco Hunan Industrial Co., Ltd, Changsha, China
Li, Zhiyuan; Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China ; Graduate School of Chinese Academy of Agricultural Science, Beijing, China
Wang, Qi; Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China ; Graduate School of Chinese Academy of Agricultural Science, Beijing, China
Ding, Anming ; Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China ; Graduate School of Chinese Academy of Agricultural Science, Beijing, China
Wang, Weifeng; Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China ; Graduate School of Chinese Academy of Agricultural Science, Beijing, China
Sun, Yuhe; Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China ; Graduate School of Chinese Academy of Agricultural Science, Beijing, China
Language :
English
Title :
Systematic investigations of the ZF-HD gene family in Tobacco reveal their multiple roles in abiotic stresses
Funding: This work was supported by the Mining and Application of Tobacco Gene Based on EMS Mutant Database (16102320200002) and the Science Foundation for Young Scholars of the Tobacco Research Institute of the Chinese Academy of Agricultural Sciences (2018B02).
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