Progress in Optimization of Agrobacterium-Mediated Transformation in Sorghum (Sorghum bicolor).

T-DNA; antibiotics; immature embryos; phenolic compounds; tissue culture; Phenols; Agrobacterium tumefaciens/genetics; Phenols/metabolism; Sorghum/genetics; Sorghum/metabolism; Sorghum/microbiology; Tissue Culture Techniques/methods; Transformation, Genetic/genetics; Agrobacterium tumefaciens; Sorghum; Tissue Culture Techniques; Transformation, Genetic; Catalysis; Molecular Biology; Spectroscopy; Computer Science Applications; Physical and Theoretical Chemistry; Organic Chemistry; Inorganic Chemistry; General Medicine

Abstract :

[en] This review archives the achievements made in the last two decades and presents a brief outline of some significant factors influencing the Agrobacterium-mediated transformation of Sorghum bicolor. Recently, progress in successful transformation has been made for this particular monocot crop through direct DNA delivery method and indirect method via Agrobacterium. However, lower transformation rate still proved to be a bottleneck in genetic modification of sorghum. An efficient Agrobacterium transformation system could be attained by optimizing the preliminary assays, comprising of explant source, growth media, antibiotics, Agrobacterium strains and agro-infection response of callus. The selection of competent strains for genetic transformation is also one of the key factors of consideration. Successful transformation is highly dependent on genome configuration of selected cultivar, where non-tannin genotype proved the best suited. Immature embryos from the field source have higher inherent adaptation chances than that of the greenhouse source. A higher concentration of Agrobacterium may damage the explant source. Utilization of anti-necrotic treatments and optimized tissue culture timeframe are the adequate strategies to lower down the effect of phenolic compounds. Appropriate selection of culture media vessels at different stages of tissue culture may also assist in a constructive manner. In conclusion, some aspects such as culture environment with medium composition, explant sources, and genotypes play an indispensable role in successful Agrobacterium-mediated sorghum transformation system.

Disciplines :

Agriculture & agronomy

Author, co-author :

Ahmed, Rana Imtiaz; Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China. imtiazheaven@yahoo.com ; Graduate School of Chinese Academy of Agricultural Science, Beijing 100081, China. imtiazheaven@yahoo.com

Ding, Anming; Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China. dinganming@caas.cn

Xie, Minmin ; Université de Liège - ULiège > TERRA Research Centre ; Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China. xieminmin@caas.cn

Kong, Yingzhen; Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China. kongyingzhen@caas.cn

Language :

English

Title :

Progress in Optimization of Agrobacterium-Mediated Transformation in Sorghum (Sorghum bicolor).

Publication date :

29 September 2018

Journal title :

International Journal of Molecular Sciences

ISSN :

1661-6596

eISSN :

1422-0067

Publisher :

MDPI AG, Basel, Switzerland

Volume :

Issue :

Pages :

2983

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://www.mdpi.com/1422-0067/19/10/2983/pdf

Funders :

NSCF - National Natural Science Foundation of China [CN]

Funding text :

This work was supported by the National Natural Science Foundation of China (3147029, 31670302, and 31600237), the National Key Technology R&D Program (2015BAD15B03) and the Elite Youth Program of Chinese Academy of Agricultural Sciences (to Y.K.).

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since 05 May 2023

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