Impact of Mesoporous Silica Functionalization Fine-Tuning on Antibiotic Uptake/Delivery and Bactericidal Activity.

[en] The mesoporous SBA-15 material was surface-functionalized with amino and carboxylic acid groups and used as a platform to investigate the interaction of these chemical groups with tetracycline, kanamycin, and ampicillin antibiotics. The interactions between the antibiotic and the functionalized surfaces were characterized using two-dimensional 1H-13C HETCOR CP MAS and FTIR spectroscopy and indicated that -COO- NH3 + bondings had been formed between chemical groups on the silica surface and drug molecules. The surface modification resulted in higher kanamycin and ampicillin loadings and a slow-release rate, and all synthesized systems showed antibacterial activity against susceptible Escherichia coli bacteria. Almost total death of bacteria was obtained using a few ppm of tetracycline- and kanamycin-loaded systems, whereas the ampicillin-loaded one showed lower bactericidal activity than free ampicillin.

Disciplines :

Chemistry

Author, co-author :

Bouchmella, Karim ; Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), P.O. Box 6192, Campinas CEP 13083-970, SP, Brazil ; Chemistry Institute (IQ), Universidade Estadual de Campinas (UNICAMP), P.O. Box 6154, Campinas CEP 13083-970, SP, Brazil ; ICGM, University Montpellier, CNRS, ENSCM, 34095 Montpellier, France

Lion, Quentin ; Université de Liège - ULiège > GIGA > GIGA Stem Cells - Medical Chemistry ; Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), P.O. Box 6192, Campinas CEP 13083-970, SP, Brazil

Gervais, Christel ; LCMCP - Laboratoire de Chimie de la Matière Condensée de Paris, Sorbonne Université, 4 place Jussieu, 75252 Paris Cedex 05, France

Cardoso, Mateus Borba ; Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), P.O. Box 6192, Campinas CEP 13083-970, SP, Brazil ; Chemistry Institute (IQ), Universidade Estadual de Campinas (UNICAMP), P.O. Box 6154, Campinas CEP 13083-970, SP, Brazil

Language :

English

Title :

Impact of Mesoporous Silica Functionalization Fine-Tuning on Antibiotic Uptake/Delivery and Bactericidal Activity.

Publication date :

04 April 2023

Journal title :

ACS Omega

eISSN :

2470-1343

Publisher :

American Chemical Society, United States

Volume :

Issue :

Pages :

12154 - 12164

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://pubs.acs.org/doi/pdf/10.1021/acsomega.2c08065

Funders :

CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
FAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulo
CAPES - Coordenação de Aperfeicoamento de Pessoal de Nível Superior
Brazilian Synchrotron Light Laboratory
LNNano - Laboratório Nacional de Nanotecnologia

Funding text :

M.B.C. thanks the support of FAPESP (Grant Nos. 2014/22322-2, 2015/25406-5, and 2021/12071-6) and the productivity research fellowship granted by CNPq (Grant No. 309107/2014-8). K.B. and Q.L. acknowledge the support of Capes (CSF-PAJT - 88887.091023/2014-00). NMR spectroscopic calculations were performed using HPC resources from GENCI-IDRIS (Grant 097535). The French Région Ile de France-SESAME program is acknowledged for financial support (700 MHz spectrometer). The authors would like to thank LNLS and LNNano for all financial support along this work. D1B and D2A SAXS beamlines (LNLS-Brazil) are acknowledged for the usage of the SAXS beamlines. We thank Jessica Fernanda Affonso de Oliveira, Agustin S. Picco, and Murilo Izidoro Santos for fruitful insights in the fluorescence spectroscopy, bactericidal experiments, and drug derivatization for spectroscopic quantification. Pr Ahmad Mehdi is acknowledged for fruitful discussions. We thank Dr. Marcio Chaim Bajgelman (LNBio - Campinas, Brazil) for providing E. coli bacteria (DH5α) for biological assays.

Available on ORBi :

since 25 April 2023

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