[en] Tannourine cedars forest, dominated by cedars (Cedrus libani, Richard), suffers from native sawfly attacks, Cephalcia tannourinensis (Chevin). The current study assesses the presence of entomopathogenic nematodes (EPNs) endemic to the forest for their potential use as biocontrol agents in an integrated pest management to control C. tannourinensis. A survey was conducted using Galleria mellonella baits in fifteen selected sites taking into consideration the cedars' different habitats. One EPN species, Steinernema feltiae, as determined by morphometric and molecular analyses, was found in one site in the forest and was tested under laboratory conditions against C. tannourinensis prepupae, causing a mortality of 64% at 250 Infective Juveniles (IJs)/prepupa. In light of previous studies, Heterorhabditis bacteriophora (commercial strain) was accordingly considered for the controlled in situ experiments where the pathogenicity of this EPN was evaluated on C. tannourinensis. A concentration of 625,000 IJs/m2 of H. bacteriophora caused a mortality rate of 85% on C. tannourinensis following inundative treatments conducted in jars buried in the forest's soil. While EPN succeeded to emerge from G. mellonella cadavers under the same conditions, no EPN emerged out of C. tannourinensis despite the high mortality percentage; also, opportunistic nematodes emerged unexpectedly out of C. tannourinensis cadavers.
Disciplines :
Environmental sciences & ecology
Author, co-author :
Rehayem, Martine ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Doct. scienc. agron. ing. biol.
Noujeim, Elise; National Center for Marine Sciences, National Council for Scientific Research-CNRS, Beirut, Lebanon
Nemer, Nabil; Department of Agricultural Engineering, Faculty of Agricultural and Food Sciences, Holy Spirit University of Kaslik, Jounieh, Lebanon
Pagès, Sylvie; DGIMI, INRA, Université de Montpellier, Montpellier, France
Ogier, Jean-Claude; DGIMI, INRA, Université de Montpellier, Montpellier, France
Thaler, Olivier; DGIMI, INRA, Université de Montpellier, Montpellier, France
Duvic, Bernard; DGIMI, INRA, Université de Montpellier, Montpellier, France
Language :
English
Title :
New insights in biocontrol strategy against Cephalcia tannourinensis, the principal insect defoliator of lebanese cedars
Acknowledgments: This research was supported by grants from the National Council for Scientific Research (CNRS) in Lebanon and by the Programme Hubert Curien CEDRE franco-libanais [grant number 32953UL]. The Tannourine Cedars Forest Nature Reserve partially supported the fieldwork.This research was supported by grants from the National Council for Scientific Research (CNRS) in Lebanon and by the Programme Hubert Curien CEDRE franco-libanais [grant number 32953UL]. The Tannourine Cedars Forest Nature Reserve partially supported the fieldwork.
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