Tomography of the cell nucleus using confocal microscopy and medium voltage electron microscopy.

Animals; Cell Nucleolus/physiology/ultrastructure; Cell Nucleus/ultrastructure; Chromatin/physiology; Chromosomes/physiology; DNA Replication/physiology; Electron Microscope Tomography/methods; Green Fluorescent Proteins/physiology; Humans; Imaging, Three-Dimensional/methods; Microscopy, Confocal/methods; Nuclear Proteins/physiology/ultrastructure

Abstract :

[en] Changes in nuclear structures are widely used by pathologists as diagnostic and prognostic indicators in cancer cells. Recent studies have demonstrated that the cell nucleus is probably the most complex organelle in the cell. It contains the genome and is the site of all related activities such as DNA repair, DNA duplication, RNA synthesis, RNA processing and RNA transport. These activities take place within dynamic three-dimensional compartments. The detailed study of these compartments requires an approach termed "cell tomography" based on 3D imaging using confocal microscopy and electron tomography. In this paper, we will first summarize the most recent findings concerning the organization of the cell nucleus. We will then describe markers used to identify molecules specific for various nuclear compartments and their use in tomography of the cell nucleus by confocal microscopy and electron tomography.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Tchelidze, Pavel

Chatron-Colliet, Aurore

Thiry, Marc ; Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire

Lalun, Natahlie

Bobichon, Helene

Ploton, Dominique

Language :

English

Title :

Tomography of the cell nucleus using confocal microscopy and medium voltage electron microscopy.

Publication date :

2009

Journal title :

Critical Reviews in Oncology/Hematology

ISSN :

1040-8428

eISSN :

1879-0461

Publisher :

Elsevier, Netherlands

Volume :

Issue :

Pages :

127-43

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 30 November 2009

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