Development of a nest-PCR for detection of Fasciola hepatica DNA in the intermediate snail host, Radix cucunorica, and the prevalence in northwestern China.
Huang, Si-Yang; Gong, Jing-Zhi; Yang, Binet al.
2019 • In Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases, 75, p. 103984
[en] Fasciolosis, a foodborne zoonotic disease, caused by Fasciola species which is considered an important problem for human health and livestock husbandry development. Snails are intermediate hosts of F. hepatica, the epidemiological surveillance of snails can evaluate the transmission risk of this disease in human and livestock. In this study, we developed a nest-polymerase chain reaction (nest-PCR) to detect the DNA of F. hepatica in Radix cucunorica, a prevalent intermediate host of this parasite in northwestern China. The nest-PCR was used to amplify a 208 bp fragment of the second internal transcribed spacer (ITS-2) of F. hepatica with two pairs of primers. The method was able to detect up to 0.16 fg genomic DNA in a 25 μL PCR reaction system even effected with high concentrations of snail DNA, and no cross reaction was observed from the genomic DNA of Paramphistomum cervi, Clonorchis sinensis, Orientobilharzia turkestanicum, Metorchis orientalis, Dicrocoelium chinensis. To evaluate the transmission risk of this disease, 409 snail samples collected from different areas of Gansu province were used to detect and analyze the transmission risk of F. hepatica in this area. Of 409 snail samples, the overall prevalence is 43.76%. The prevalence was 92.75% in Gannan Tibetan Autonomous Prefecture, while no snail was positive for F. hepatica in Linxia Hui Autonomous Prefecture. The nest-PCR was firstly used to detect the infection of F. hepatica in snail. It is a novel, useful and convenient method with high sensitivity and specificity. This study is the first report about the epidemiological surveillance of snail infection by F. hepatica in northwestern China, which will help to evaluate the transmission risk of F. hepatica in northwestern China.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Huang, Si-Yang; Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Jiangsu Co-innovation Center for Prevention, Control of Important Animal Infectious Diseases and Zoonosis, Jiangsu Key Laboratory of Zoonosis, Yangzhou, Jiangsu Province 225009, PR China, State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China. Electronic address: siyang.huang@hotmail.com
Gong, Jing-Zhi; Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Jiangsu Co-innovation Center for Prevention, Control of Important Animal Infectious Diseases and Zoonosis, Jiangsu Key Laboratory of Zoonosis, Yangzhou, Jiangsu Province 225009, PR China
Yang, Bin ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) ; Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Jiangsu Co-innovation Center for Prevention, Control of Important Animal Infectious Diseases and Zoonosis, Jiangsu Key Laboratory of Zoonosis, Yangzhou, Jiangsu Province 225009, PR China
Fan, Yi-Min; Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Jiangsu Co-innovation Center for Prevention, Control of Important Animal Infectious Diseases and Zoonosis, Jiangsu Key Laboratory of Zoonosis, Yangzhou, Jiangsu Province 225009, PR China
Yao, Na; Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Jiangsu Co-innovation Center for Prevention, Control of Important Animal Infectious Diseases and Zoonosis, Jiangsu Key Laboratory of Zoonosis, Yangzhou, Jiangsu Province 225009, PR China
Wang, Chun-Ren ; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China
Language :
English
Title :
Development of a nest-PCR for detection of Fasciola hepatica DNA in the intermediate snail host, Radix cucunorica, and the prevalence in northwestern China.
Publication date :
November 2019
Journal title :
Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases
This work was supported by the National Key Research and Development Program of China (Grant No. 2017YFD0501200 ), the “ National Key Basic Research Program (973 Program) of China” (Grant No. 2015CB150300 ), and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (Veterinary Medicine);
Alba, A., Vazquez, A.A., Hernandez, H., Sanchez, J., Marcet, R., Figueredo, M., Sarracent, J., Fraga, J., A multiplex PCR for the detection of Fasciola hepatica in the intermediate snail host Galba cubensis. Vet. Parasitol. 211 (2015), 195–200.
Becker, A.C., Kraemer, A., Epe, C., Strube, C., Sensitivity and efficiency of selected coproscopical methods-sedimentation, combined zinc sulfate sedimentation-flotation, and McMaster method. Parasitol. Res. 115 (2016), 2581–2587.
Caron, Y., Rondelaud, D., Losson, B., The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola sp. Parasitol. Res. 103 (2008), 735–744.
Caron, Y., Righi, S., Lempereur, L., Saegerman, C., Losson, B., An optimized DNA extraction and multiplex PCR for the detection of Fasciola sp. in lymnaeid snails. Vet. Parasitol. 178 (2011), 93–99.
Correa, A.C., Escobar, J.S., Durand, P., Renaud, F., David, P., Jarne, P., Pointier, J.P., Hurtrez-Bousses, S., Bridging gaps in the molecular phylogeny of the Lymnaeidae (Gastropoda: Pulmonata), vectors of fascioliasis. BMC Evol. Biol., 10, 2010, 381.
Cucher, M.A., Carnevale, S., Prepelitchi, L., Labbe, J.H., Wisnivesky-Colli, C., PCR diagnosis of Fasciola hepatica in field-collected Lymnaea columella and Lymnaea viatrix snails. Vet. Parasitol. 137 (2006), 74–82.
Dreyfuss, G., Vignoles, P., Rondelaud, D., Fasciola hepatica: epidemiological surveillance of natural watercress beds in Central France. Parasitol. Res. 95 (2005), 278–282.
George, S.D., Vanhoff, K., Baker, K., Lake, L., Rolfe, P.F., Seewald, W., Emery, D.L., Application of a coproantigen ELISA as an indicator of efficacy against multiple life stages of Fasciola hepatica infections in sheep. Vet. Parasitol. 246 (2017), 60–69.
Happich, F.A., Boray, J.C., Quantitative diagnosis of chronic fasciolosis. 1. Comparative studies on quantitative faecal examinations for chronic Fasciola hepatica infection in sheep. Aust. Vet. J. 45 (1969), 326–328.
Hong, S.T., Choi, M.H., Kim, C.H., Chung, B.S., Ji, Z., The Kato-Katz method is reliable for diagnosis of Clonorchis sinensis infection. Diagn. Microbiol. Infect. Dis. 47 (2003), 345–347.
Huang, W.Y., He, B., Wang, C.R., Zhu, X.Q., Characterisation of Fasciola species from mainland China by ITS-2 ribosomal DNA sequence. Vet. Parasitol. 120 (2004), 75–83.
Jones, R.A., Brophy, P.M., Davis, C.N., Davies, T.E., Emberson, H., Rees Stevens, P., Williams, H.W., Detection of Galba truncatula, Fasciola hepatica and Calicophoron daubneyi environmental DNA within water sources on pasture land, a future tool for fluke control?. Parasit. Vectors, 11, 2018, 342.
Kaplan, R.M., Dame, J.B., Reddy, G.R., Courtney, C.H., The prevalence of Fasciola hepatica in its snail intermediate host determined by DNA probe assay. Int. J. Parasitol. 27 (1997), 1585–1593.
Lin, R.Q., Dong, S.J., Nie, K., Wang, C.R., Song, H.Q., Li, A.X., Huang, W.Y., Zhu, X.Q., Sequence analysis of the first internal transcribed spacer of rDNA supports the existence of the intermediate Fasciola between F. hepatica and F. gigantica in mainland China. Parasitol. Res. 101 (2007), 813–817.
Magalhaes, K.G., Passos, L.K., Carvalho Odos, S., Detection of Lymnaea columella infection by Fasciola hepatica through multiplex-PCR. Mem. Inst. Oswaldo Cruz 99 (2004), 421–424.
Magalhaes, K.G., Jannotti-Passos, L.K., Caldeira, R.L., Berne, M.E., Muller, G., Carvalho, O.S., Lenzi, H.L., Isolation and detection of Fasciola hepatica DNA in Lymnaea viatrix from formalin-fixed and paraffin-embedded tissues through multiplex-PCR. Vet. Parasitol. 152 (2008), 333–338.
Martinez-Perez, J.M., Robles-Perez, D., Rojo-Vazquez, F.A., Martinez-Valladares, M., Comparison of three different techniques to diagnose Fasciola hepatica infection in experimentally and naturally infected sheep. Vet. Parasitol. 190 (2012), 80–86.
Mas-Coma, S., Bargues, M.D., Valero, M.A., Fascioliasis and other plant-borne trematode zoonoses. Int. J. Parasitol. 35 (2005), 1255–1278.
Mas-Coma, S., Valero, M.A., Bargues, M.D., Climate change effects on trematodiases, with emphasis on zoonotic fascioliasis and schistosomiasis. Vet. Parasitol. 163 (2009), 264–280.
Mezo, M., Gonzalez-Warleta, M., Ubeira, F.M., The use of MM3 monoclonal antibodies for the early immunodiagnosis of ovine fascioliasis. J. Parasitol. 93 (2007), 65–72.
Mohammad Alizadeh, A.H., Roshani, M., Lahmi, F., Davoodi, N.A., Rostami Nejad, M., Seyyedmajidi, M.R., Zali, M.R., Cholangiocarcinoma in magnetic resonance cholangiopancreatography and fascioliasis in endoscopic ultrasonography. Case Rep Gastroenterol 5 (2011), 569–577.
Molloy, J.B., Anderson, G.R., The distribution of Fasciola hepatica in Queensland, Australia, and the potential impact of introduced snail intermediate hosts. Vet. Parasitol. 137 (2006), 62–66.
Nawrocki, C.C., Kiatsopit, N., Namsanor, J., Sithithaworn, P., Carlton, E.J., Culture of fecal indicator bacteria from snail intestinal tubes as a tool for assessing the risk of Opisthorchis viverrini infection in Bithynia snail habitat. Parasit. Vectors, 12, 2019, 66.
Nyindo, M., Lukambagire, A.H., Fascioliasis: an ongoing zoonotic trematode infection. Biomed. Res. Int., 2015, 2015, 786195.
Ollerenshaw, C.B., Some observations on the epidemiology of fascioliasis in relation to the timing of molluscicide applications in the control of the disease. Vet. Rec. 88 (1971), 152–164.
Piedrafita, D., Spithill, T.W., Smith, R.E., Raadsma, H.W., Improving animal and human health through understanding liver fluke immunology. Parasite Immunol. 32 (2010), 572–581.
Rathinasamy, V., Hosking, C., Tran, L., Kelley, J., Williamson, G., Swan, J., Elliott, T., Rawlin, G., Beddoe, T., Spithill, T.W., Development of a multiplex quantitative PCR assay for detection and quantification of DNA from Fasciola hepatica and the intermediate snail host, Austropeplea tomentosa, in water samples. Vet. Parasitol. 259 (2018), 17–24.
Schweizer, G., Meli, M.L., Torgerson, P.R., Lutz, H., Deplazes, P., Braun, U., Prevalence of Fasciola hepatica in the intermediate host Lymnaea truncatula detected by real time TaqMan PCR in populations from 70 Swiss farms with cattle husbandry. Vet. Parasitol. 150 (2007), 164–169.
Spithill, T.W., Dalton, J.P., Progress in development of liver fluke vaccines. Parasitol. Today 14 (1998), 224–228.
Takeuchi-Storm, N., Denwood, M., Petersen, H.H., Enemark, H.L., Stensgaard, A.S., Sengupta, M.E., Beesley, N.J., Hodgkinson, J., Williams, D., Thamsborg, S.M., Patterns of Fasciola hepatica infection in Danish dairy cattle: implications for on-farm control of the parasite based on different diagnostic methods. Parasit. Vectors, 11, 2018, 674.
Zhang, J.L., Zhang, C.Q., Gao, L.M., Yang, J.B., Li, H.T., Natural hybridization origin of Rhododendron agastum (Ericaceae) in Yunnan, China: inferred from morphological and molecular evidence. J. Plant Res. 120 (2007), 457–463.
Zhang, X.X., Feng, S.Y., Ma, J.G., Zheng, W.B., Yin, M.Y., Qin, S.Y., Zhou, D.H., Zhao, Q., Zhu, X.Q., Seroprevalence and risk factors of fascioliasis in yaks, Bos grunniens, from three counties of Gansu Province, China. Korean J Parasitol 55 (2017), 89–93.
Zhang, J.L., Si, H.F., Zhou, X.Z., Shang, X.F., Li, B., Zhang, J.Y., High prevalence of fasciolosis and evaluation of the efficacy of anthelmintics against Fasciola hepatica in buffaloes in Guangxi, China. Int J Parasitol Parasites Wildl 8 (2019), 82–87.
