[en] Cryptosporidium is a zoonotic parasite that causes diarrhea in a broad range of animals, including deer. Little is known about the prevalence and genotype of Cryptosporidium spp. in Père David's deer. In this study, 137 fecal samples from Père David's deer were collected between July 2017 and August 2018 in the Dafeng Reserve and analyzed for Cryptosporidium spp. by nested-PCR based on the small subunit ribosomal RNA (SSU rRNA) gene, followed by sequence analyses to determine the species. The 60 kDa glycoprotein (gp60) gene was used to characterize Cryptosporidium spp. Among 137 samples, 2 (1.46%) were positive for Cryptosporidium spp. according to SSU rRNA gene sequencing results. Both samples belonged to the Cryptosporidium deer genotype, with two nucleotide deletions and one nucleotide substitution. The prevalence data and molecular characterization of this study provide basic knowledge for controlling and preventing Cryptosporidium infections in Père David's deer in this area.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Huang, Si-Yang ; College of Veterinary Medicine, Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu Province, PR China
Fan, Yi-Min; College of Veterinary Medicine, Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu Province, PR China
Yang, Yi; College of Veterinary Medicine, Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu Province, PR China
Ren, Yi-Jun; Dafeng Elk National Natural Reserve, Yancheng, Jiangsu Province, PR China
Gong, Jing-Zhi; College of Veterinary Medicine, Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu Province, PR China
Yao, Na; College of Veterinary Medicine, Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu Province, PR China
Yang, Bin ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) ; College of Veterinary Medicine, Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu Province, PR China
Language :
English
Title :
Prevalence and molecular characterization of Cryptosporidium spp. in Père David's deer (Elaphurus davidianus) in Jiangsu, China.
Alves M, Xiao L, Sulaiman I, Lal AA, Matos O, Antunes F. Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal. J Clin Microbiol 2003; 41(6): 2744-2747. http://dx.doi.org/10.1128/JCM.41.6.2744-2747.2003. PMid:12791920.
Baroudi D, Hakem A, Adamu H, Amer S, Khelef D, Adjou K, et al. Zoonotic Cryptosporidium species and subtypes in lambs and goat kids in Algeria. Parasit Vectors 2018; 11(1): 582. http://dx.doi.org/10.1186/s13071-018-3172-2. PMid:30400983.
Cinque K, Stevens MA, Haydon SR, Jex AR, Gasser RB, Campbell BE. Investigating public health impacts of deer in a protected drinking water supply watershed. Water Sci Technol 2008; 58(1): 127-132. http://dx.doi.org/10.2166/wst.2008.632. PMid:18653946.
Deng MQ, Cliver DO. Improved immunofluorescence assay for detection of Giardia and Cryptosporidium from asymptomatic adult cervine animals. Parasitol Res 1999; 85(8-9): 733-736. http://dx.doi.org/10.1007/s004360050623. PMid:10431741.
Ding Y, Ding J, Li P, Zhu J. Strategy study of wild Pere David deer (Elaphurus davidianus) population development in China. J Jiangsu Forestry Sci Technol 2018; 45: 49-51.
Feng Y, Alderisio KA, Yang W, Blancero LA, Kuhne WG, Nadareski CA, et al. Cryptosporidium genotypes in wildlife from a New York watershed. Appl Environ Microbiol 2007a; 73(20): 6475-6483. http://dx.doi.org/10.1128/AEM.01034-07. PMid:17720824.
Feng Y, Karna SR, Dearen TK, Singh DK, Adhikari LN, Shrestha A, et al. Common occurrence of a unique Cryptosporidium ryanae variant in zebu cattle and water buffaloes in the buffer zone of the Chitwan National Park, Nepal. Vet Parasitol 2012; 185(2-4): 309-314. https://doi.org/10.1016/j.vetpar.2011.09.025.
Feng Y, Ortega Y, He G, Das P, Xu M, Zhang X, et al. Wide geographic distribution of Cryptosporidium bovis and the deer-like genotype in bovines. Vet Parasitol 2007b; 144(1-2): 1-9. http://dx.doi.org/10.1016/j.vetpar.2006.10.001. PMid:17097231.
García-Presedo I, Pedraza-Díaz S, González-Warleta M, Mezo M, Gómez-Bautista M, Ortega-Mora LM, et al. The first report of Cryptosporidium bovis, C. ryanae and Giardia duodenalis sub-assemblage A-II in roe deer (Capreolus capreolus) in Spain. Vet Parasitol 2013; 197(3-4): 658-664. http://dx.doi.org/10.1016/j.vetpar.2013.07.002. PMid:23890824.
Huang J, Zhang Z, Zhang Y, Yang Y, Zhao J, Wang R, et al. Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in deer in Henan and Jilin, China. Parasit Vectors 2018; 11(1): 239. http://dx.doi.org/10.1186/s13071-018-2813-9. PMid:29650036.
Huang JY, Yue DY, Qi M, Wang RJ, Zhao JF, Li JQ, et al. Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in dairy cattle in Ningxia, northwestern China. BMC Vet Res 2014; 10(1): 292. http://dx.doi.org/10.1186/s12917-014-0292-6. PMid:25488627.
Jellison KL, Lynch AE, Ziemann JM. Source tracking identifies deer and geese as vectors of human-infectious Cryptosporidium genotypes in an urban/suburban watershed. Environ Sci Technol 2009; 43(12): 4267-4272. http://dx.doi.org/10.1021/es900081m. PMid:19603633.
Kato S, Yanagawa Y, Matsuyama R, Suzuki M, Sugimoto C. Molecular identification of the Cryptosporidium deer genotype in the Hokkaido sika deer (Cervus nippon yesoensis) in Hokkaido, Japan. Parasitol Res 2016; 115(4): 1463-1471. http://dx.doi.org/10.1007/s00436-015-4880-6. PMid:26687968.
Koehler AV, Haydon SR, Jex AR, Gasser RB. Cryptosporidium and Giardia taxa in faecal samples from animals in catchments supplying the city of Melbourne with drinking water (2011 to 2015). Parasit Vectors 2016; 9:315. https://dx.doi.org/10.1186%2 Fs13071-016-1607-1.
Kotková M, Nemejc K, Sak B, Hanzal V, Kvetonova D, Hlaskova L, et al. Cryptosporidium ubiquitum, C. muris and Cryptosporidium deer genotype in wild cervids and caprines in the Czech Republic. Folia Parasitol (Praha) 2016; 63: 003. https://doi.org/10.14411/fp.2016.003.
Li N, Xiao LH, Alderisio K, Elwin K, Cebelinski E, Chalmers R, et al. Subtyping Cryptosporidium ubiquitum, a Zoonotic Pathogen Emerging in Humans. Emerg Infect Dis 2014; 20(2): 217-224. http://dx.doi.org/10.3201/eid2002.121797. PMid:24447504.
Parsons MB, Travis D, Lonsdorf EV, Lipende I, Roellig DMA, Kamenya S, et al. Epidemiology and molecular characterization of Cryptosporidium spp. in humans, wild primates, and domesticated animals in the Greater Gombe Ecosystem, Tanzania. PLoS Negl Trop Dis 2015; 9(2): e0003529. http://dx.doi.org/10.1371/journal.pntd.0003529. PMid:25700265.
Perz JF, Le Blancq SM. Cryptosporidium parvum infection involving novel genotypes in wildlife from lower New York State. Appl Environ Microbiol 2001; 67(3): 1154-1162. http://dx.doi.org/10.1128/AEM.67.3.1154-1162.2001. PMid:11229905.
Robinson G, Chalmers RM, Stapleton C, Palmer SR, Watkins J, Francis C, et al. A whole water catchment approach to investigating the origin and distribution of Cryptosporidium species. J Appl Microbiol 2011; 111(3): 717-730. http://dx.doi.org/10.1111/j.1365-2672.2011.05068.x. PMid:21649804.
Ryan U, Fayer R, Xiao L. Cryptosporidium species in humans and animals: current understanding and research needs. Parasitology 2014; 141(13): 1667-1685. http://dx.doi.org/10.1017/S0031182014001085. PMid:25111501.
Santin M, Fayer R. Enterocytozoon bieneusi, Giardia, and Cryptosporidium infecting white-tailed deer. J Eukaryot Microbiol 2015; 62(1): 34-43. http://dx.doi.org/10.1111/jeu.12155. PMid:25066778.
Tanriverdi S, Blain JC, Deng B, Ferdig MT, Widmer G. Genetic crosses in the apicomplexan parasite Cryptosporidium parvum define recombination parameters. Mol Microbiol 2007; 63(5): 1432-1439. http://dx.doi.org/10.1111/j.1365-2958.2007.05594.x. PMid:17302818.
Wang R, Wang J, Sun M, Dang H, Feng Y, Ning C, et al. Molecular characterization of the Cryptosporidium cervine genotype from a sika deer (Cervus nippon Temminck) in Zhengzhou, China and literature review. Parasitol Res 2008; 103(4): 865-869. http://dx.doi. org/10.1007/s00436-008-1069-2. PMid:18575889.
Wells B, Shaw H, Hotchkiss E, Gilray J, Ayton R, Green J, et al. Prevalence, species identification and genotyping Cryptosporidium from livestock and deer in a catchment in the Cairngorms with a history of a contaminated public water supply. Parasit Vectors 2015; 8(1): 66. http://dx.doi.org/10.1186/s13071-015-0684-x. PMid:25650114.
Xiao L, Sulaiman IM, Ryan UM, Zhou L, Atwill ER, Tischler ML, et al. Host adaptation and host-parasite co-evolution in Cryptosporidium: implications for taxonomy and public health. Int J Parasitol 2002; 32(14): 1773-1785. http://dx.doi.org/10.1016/S0020-7519(02)00197-2. PMid:12464424.
Zhang XX, Cong W, Ma JG, Lou ZL, Zheng WB, Zhao Q, et al. First report of Cryptosporidium canis in farmed Arctic foxes (Vulpes lagopus) in China. Parasit Vectors 2016; 9(1): 126. http://dx.doi.org/10.1186/s13071-016-1396-6. PMid:26934975.
Zhao GH, Du SZ, Wang HB, Hu XF, Deng MJ, Yu SK, et al. First report of zoonotic Cryptosporidium spp., Giardia intestinalis and Enterocytozoon bieneusi in golden takins (Budorcas taxicolor bedfordi). Infect Genet Evol 2015; 34: 394-401. http://dx.doi.org/10.1016/j. meegid.2015.07.016. PMid:26190449.
Zhao GH, Ren WX, Gao M, Bian QQ, Hu B, Cong MM, et al. Genotyping Cryptosporidium andersoni in cattle in Shaanxi Province, Northwestern China. PLoS One 2013; 8(4): e60112. http://dx.doi.org/10.1371/journal.pone.0060112. PMid:23560072.
