A three-year evolution and comparison of the bla CTX-M genes in pathogenic and non-pathogenic Escherichia coli isolated from young diarrheic and septicaemic calves in Belgium
[en] Escherichia coli producing Extended-Spectrum-β-Lactamases (ESBL) are a major public health hazard worldwide.
The most frequent ESBL belong to the CTX-M family. This study follows their prevalence in pathogenic and nonpathogenic ESBL-producing E. coli isolated from young diarrheic and septicaemic calves over three calving
seasons. The triplex PCR targeted three main groups: CTX-M-1, CTX-M-2 and CTX-M-9. Of the 394 isolates
studied, 388 (98.5%) were positive, with a majority of CTX-M-1 (243, 61.7%), following by CTX-M-9 (74, 18.8%)
and CTX-M-2 (64, 16.2%). The progressive decrease of ESBL-resistance of pathogenic E. coli is not linked to any
shift in genetic background, blaCTX-M genes still present in 99% of the isolates, or to the proportion of the three
CTX-M groups. Moreover, no significant difference was observed in the CTX-M content between pathogenic and
non-pathogenic E. coli.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Guérin, Virginie; Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, University of Liège, Bacteriology, Liège, Belgium ; UMR3525, Current affiliation: Unité EERA, Institut Pasteur, APHP, Université Paris Saclay, Paris, France ; CNRS, Roux, Paris, France
Mercuri, Paola ; Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Lucas, Pierrick; Anses sequencing platform, ANSES, Ploufragan-Plouzané-Niort Laboratory, Ploufragan, France
Blanchard, Yannick; Anses sequencing platform, ANSES, Ploufragan-Plouzané-Niort Laboratory, Ploufragan, France
Saulmont, Marc; Regional Animal Health and Identification Association (ARSIA), Ciney, Belgium
Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI)
Thiry, Damien ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Bactériologie vétérinaire et maladies bactériennes animales
Language :
English
Title :
A three-year evolution and comparison of the bla CTX-M genes in pathogenic and non-pathogenic Escherichia coli isolated from young diarrheic and septicaemic calves in Belgium
Argudín, M.A., Deplano, A., Meghraoui, A., Dodémont, M., Heinrichs, A., Denis, O., et al. Bacteria from animals as a pool of antimicrobial resistance genes. Antibiotics, 6, 2017, 12, 10.3390/antibiotics6020012.
Association Régionale de Santé et d’’Identification Animales (ARSIA), Antibiogrammes Rapport d'activités et résultats de l'ARSIA. 6th edition, 2020.
Beutin, L., Montenegro, M.A., Orskov, I., Orskov, F., Prada, J., Zimmermann, S., Stephan, R., Close association of verotoxin (Shiga-like toxin) production with enterohemolysin production in strains of Escherichia coli. J. Clin. Microbiol. 27 (1989), 2559–2564.
Bevan, E.R., Jones, A.M., Hawkey, P.M., Global epidemiology of CTX-M β-lactamases: temporal and geographical shifts in genotype. J. Antimicrob. Chemother. 72 (2017), 2145–2155.
Castanheira, M., Simner, P.J., Bradford, P.A., Extended-spectrum β-lactamases: an update on their characteristics, epidemiology and detection. JAC Antimicrob. Resist., 3, 2021, dlab092, 10.1093/jacamr/dlab092.
D'Andrea, M.M., Arena, F., Pallecchi, L., Rossolini, G.M., CTX-M-type β-lactamases: a successful story of antibiotic resistance. Int. J. Med. Microbiol. 303 (2013), 305–317.
ECDC, (European Centre for Disease Prevention and Control). Antimicrobial Resistance in the EU/EEA (EARS-Net) - Annual Epidemiological Report 2019. 2020, Stockholm, ECDC.
EFSA (European Food Safety Authority) and ECDC (European Centre for Disease Prevention and Control), The European Union Summary Report on Antimicrobial Resistance in zoonotic and indicator bacteria from humans, animals and food in 2017/2018. EFSA J., 18(6007), 2020, 166, 10.2903/j.efsa.2020.6007.
Elmonir, W., Shalaan, S., Tahoun, A., Mahmoud, S.F., Remela, E.M.A., Eissa, R., et al. Prevalence, antimicrobial resistance, and genotyping of Shiga toxin-producing Escherichia coli in foods of cattle origin, diarrheic cattle, and diarrheic humans in Egypt. Gut. Pathog., 13, 2021, 8, 10.1186/s13099-021-00402-y.
Ewers, C., Bethe, A., Semmler, T., Guenther, S., Wieler, L.H., Extended-spectrum β-lactamase-producing and AmpC-producing Escherichia coli from livestock and companion animals, and their putative impact on public health: a global perspective. Clin. Microbiol. Infect. 18 (2012), 646–655.
Guérin, V., Thiry, D., Lucas, P., Blanchard, Y., Cawez, F., Mercuri, P.S., et al. Identification of β-lactamase-encoding (bla) genes in phenotypically β-lactam-resistant Escherichia coli isolated from young calves in Belgium. Microb. Drug Resist. 27 (2021), 1578–1584.
Guérin, V., Farchi, A., Thiry, D., Cawez, F., Mercuri, P.S., Galleni, M., et al. Seven-year evolution of β-lactam resistance phenotypes in Escherichia coli isolated from young diarrheic and septicaemic calves in Belgium. Vet. Sci., 9, 2022, 45, 10.3390/vetsci9020045.
Lahlaoui, H., Ben Haj Khalifa, A., Ben, Moussa M., Epidemiology of Enterobacteriaceae producing CTX-M type extended spectrum β-lactamase (ESBL). Med. Mal. Infect. 44 (2014), 400–404.
Mainil, J.G., Fairbrother, J.M., Pathogenic Escherichia coli in domestic mammals and birds. Pathogenic Escherichia coli: Molecular and Cellular Microbiology. Morabito S, First edition, 2014, Horizon Scientific Press et Caister Academic Press, Norwich, England, UK, 68.
Markey, B.K., Leonard, F.C., Archambault, M., Cullinane, A., Maguire, D., Clinical Veterinary Microbiology. second ed, 2013, Elsevier Ltd.
Naas, T., Oueslati, S., Bonnin, R.A., Dabos, M.L., Zavala, A., Dortet, L., et al. Beta-lactamase DataBase (BLDB) – structure and function. J. Enzyme Inhib. Med. Chem. 32 (2017), 917–919.
Ogutu, J.O., Zhang, Q., Huang, Y., Yan, H., Su, L., Gao, B., et al. Development of a multiplex PCR system and its application in detection of blaSHV, blaTEM, blaCTX-M-1, blaCTX-M-9 and blaOXA-1 group genes in clinical Klebsiella pneumoniae and Escherichia coli strains. J. Antibiot (Tokyo) 68 (2015), 725–733.
Pitout, J.D.D., Hossain, A., Hanson, N.D., Phenotypic and molecular detection of CTX-M-beta-lactamases produced by Escherichia coli and Klebsiella spp. J. Clin. Microbiol. 42 (2004), 5715–5721.
Poyart, C., Mugnier, P., Quesne, G., Berche, P., Trieu-Cuot, P., A novel extended-spectrum TEM-type beta-lactamase (TEM-52) associated with decreased susceptibility to moxalactam in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 42 (1998), 108–113, 10.1128/AAC.42.1.108.
Robinson, T.P., Bu, D.P., Carrique-Mas, J., Fèvre, E.M., Gilbert, M., Grace, D., et al. Antibiotic resistance is the quintessential one health issue. Trans. R. Soc. Trop. Med. Hyg. 110 (2016), 377–380.
Royal Decree of Belgium (French version), Arrêté Royal du 21 juillet 2016. Arrêté royal relatif aux conditions d'utilisation des médicaments par les médecins vétérinaires et par les responsables des animaux. Available (in French) at: https://www.ejustice.just.fgov.be/cgi/article_body.pl?language=fr&caller=summary&pub_date=16-07-29&numac=2016024152, 2016 Last consulted on September 7, 2022.
WHO (World Health Organization), Global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics. 2017, WHO Available at : https://www.who.int/medicines/publications/WHO-PPL-Short_Summary_25Feb-ET_NM_WHO.pdf?ua=1 Last consulted on December 16, 2021.
Zankari, E., Hasman, H., Cosentino, S., Vestergaard, M., Rasmussen, S., Lund, O., et al. Identification of acquired antimicrobial resistance genes. J. Antimicrob. Chemother., 10. PMID, 2012, 22782487, 10.1093/jac/dks261.
