[en] Pectins are major components of the primary plant cell wall. They can be both methylesterified and acetylesterified and de-esterification occurs by specific esterases. Proteins extracted by NaCl treatment from root cell walls of two chicory varieties (Cichorium intybus L. cv. Nausica and Arancha) sampled in an experimental field every 2 weeks between July 2002 and January 2003 were analysed by isoelectrofocalization, semi-denaturing SDS-PAGE, and quantitative assays for their esterase activity. Zymograms showed that chicory root pectin methylesterases belong to a multigene family. The isoelectric points of the pectin methylesterase isoforms ranged from pI 3.8 to pI 9.0. Concerning acetylesterases, only acidic isoforms between pI 4.1 and pI 5.2 were observed, but a large polymorphism of this class of enzymes could be identified in one variety. The results indicate that the root pectin methylesterase activity of the Nausica variety was correlated with ambient temperature, while no significant effect of temperature could be detected on any acetylesterase isoform.
Thonar, Cécile ; Université de Liège - ULiège > Département GxABT > Plant Sciences ; Unité de Recherche en Biologie cellulaire végétale, Facultés Universitaires Notre-Dame de la Paix, Rue de Bruxelles 61, B-5000 Namur, Belgium
Liners, Françoise; Unité de Recherche en Biologie Cellulaire Végétale, Facultés Universitaires Notre-Dame de la Paix, B-5000 Namur, Belgium
Van Cutsem, Pierre; Unité de Recherche en Biologie Cellulaire Végétale, Facultés Universitaires Notre-Dame de la Paix, B-5000 Namur, Belgium
Language :
English
Title :
Polymorphism and modulation of cell wall esterase enzyme activities in the chicory root during the growing season.
The authors are grateful to Nicolas Dauchot and Mallory Draye for helpful discussions and valuable technical advice, to Pierre Cambier for excellent technical help and to Francxcoise Lonnoy for the layout of the figures. This work was supported by Direction Générale de l’Agriculture, Ministère de la Région Wallonne, Belgium.
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