Article (Scientific journals)
Study of Strawberry Notch homolog 1 and 2 expression in human glioblastoma.
Coppieters, Natacha; Scalisi, Joshua; Di Gregorio, Marina et al.
2023In Journal of Neuro-Oncology
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Keywords :
Glioblastoma; Patient-derived cells; Strawberry-Notch homologs 1 and 2; Oncology; Neurology; Neurology (clinical); Cancer Research
Abstract :
[en] [en] PURPOSE: In this work, we aimed to comprehensively document the expression of Strawberry Notch homolog (SBNO) 1 and 2 in glioblastoma (GBM) tissue and patient-derived GBM stem-like cell (GSC) cultures. METHODS: We investigated SBNO1 and SBNO2 expression at the RNA and protein levels in glioma patient tissue and GSCs, respectively by performing immunostainings and qPCR analyses. We also used publicly-available datasets for assessing SBNO1 and SBNO2 gene expression and related copy number alterations. We used lentiviral transduction of SBNO2 to analyze the effect of its expression in patient-derived GSCs. RESULTS: We observed that SBNO2 expression is increased in GBM tissue samples compared to non tumoral brain, or lower-grade gliomas, whereas SBNO1 expression remains unchanged. We hypothesized that such SBNO2 high expression might be linked to copy-number alterations at the level of the 19p13 chromosome section. We located SBNO1 and SBNO2 in different subcellular compartments. Finally, we observed that SBNO2 overexpression induces different phenotypes in different patient-derived GSCs. CONCLUSION: These results provide the first characterization of SBNO1 and SBNO2 expression in glioma tissue, and indicate SBNO2 as highly expressed in GBM.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Coppieters, Natacha;  Laboratory of Nervous System Diseases and Therapy, GIGA Neuroscience, GIGA Institute, University of Liège, Liège, Belgium
Scalisi, Joshua ;  Université de Liège - ULiège > GIGA
Di Gregorio, Marina  ;  Université de Liège - ULiège > Soins primaires et santé
Leparc, Louise ;  Université de Liège - ULiège > Faculté de Médecine > Master sc. bioméd., à fin.
Velazquez Saez, Laetitia ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Lombard, Arnaud  ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de neurochirurgie
Rogister, Bernard  ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de neurologie
Neirinckx, Virginie ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques
Language :
Title :
Study of Strawberry Notch homolog 1 and 2 expression in human glioblastoma.
Publication date :
25 January 2023
Journal title :
Journal of Neuro-Oncology
Publisher :
Springer, United States
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Neurological Foundation of New Zealand [NZ]
Télévie [BE]
Fondation Léon Fredericq [BE]
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Funding text :
We are invaluably grateful for the funding support provided by the the F.R.S.-F.N.R.S., Télévie, the Fondation Hospitalo-Universitaire Léon Frédéricq (Belgium), and the Neurological Foundation (New Zealand). NC is supported by the Neurological Fondation of New Zealand, JS is supported by a Télévie grant, and AL is Post-Doctoral Master Specialist (F.N.R.S.).
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since 26 February 2023


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