Article (Scientific journals)
Role of Alternative Elicitor Transporters in the Onset of Plant Host Colonization by Streptomyces scabiei 87-22
Francis, Isolde M.; Bergin, Danica; Deflandre, Benoit et al.
2023In Biology, 12 (2), p. 234
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Keywords :
General Agricultural and Biological Sciences; General Immunology and Microbiology; General Biochemistry, Genetics and Molecular Biology
Abstract :
[en] Plant colonization by Streptomyces scabiei, the main cause of common scab disease on root and tuber crops, is triggered by cello-oligosaccharides, cellotriose being the most efficient elicitor. The import of cello-oligosaccharides via the ATP-binding cassette (ABC) transporter CebEFG-MsiK induces the production of thaxtomin phytotoxins, the central virulence determinants of this species, as well as many other metabolites that compose the ‘virulome’ of S. scabiei. Homology searches revealed paralogues of the CebEFG proteins, encoded by the cebEFG2 cluster, while another ABC-type transporter, PitEFG, is encoded on the pathogenicity island (PAI). We investigated the gene expression of these candidate alternative elicitor importers in S. scabiei 87-22 upon cello-oligosaccharide supply by transcriptomic analysis, which revealed that cebEFG2 expression is highly activated by both cellobiose and cellotriose, while pitEFG expression was barely induced. Accordingly, deletion of pitE had no impact on virulence and thaxtomin production under the conditions tested, while the deletion of cebEFG2 reduced virulence and thaxtomin production, though not as strong as the mutants of the main cello-oligosaccharide transporter cebEFG1. Our results thus suggest that both ceb clusters participate, at different levels, in importing the virulence elicitors, while PitEFG plays no role in this process under the conditions tested. Interestingly, under more complex culture conditions, the addition of cellobiose restored thaxtomin production when both ceb clusters were disabled, suggesting the existence of an additional mechanism that is involved in sensing or importing the elicitor of the onset of the pathogenic lifestyle of S. scabiei.
Disciplines :
Microbiology
Author, co-author :
Francis, Isolde M. ;  Department of Biology, California State University, Bakersfield, CA 93311-1022, USA
Bergin, Danica;  Department of Biology, California State University, Bakersfield, CA 93311-1022, USA
Deflandre, Benoit 
Gupta, Sagar ;  Department of Biology, California State University, Bakersfield, CA 93311-1022, USA
Salazar, Joren J. C.;  Department of Biology, California State University, Bakersfield, CA 93311-1022, USA
Villagrana, Richard ;  Department of Biology, California State University, Bakersfield, CA 93311-1022, USA
Stulanovic, Nudzejma  ;  Université de Liège - ULiège > Integrative Biological Sciences (InBioS)
Ribeiro Monteiro, Silvia  
Kerff, Frédéric  ;  Université de Liège - ULiège > Département des sciences de la vie > Centre d'Ingénierie des Protéines (CIP)
Loria, Rosemary;  Department of Plant Pathology, University of Florida, Gainesville, FL 32611-0180, USA
Rigali, Sébastien  ;  Université de Liège - ULiège > Département des sciences de la vie
Language :
English
Title :
Role of Alternative Elicitor Transporters in the Onset of Plant Host Colonization by Streptomyces scabiei 87-22
Publication date :
01 February 2023
Journal title :
Biology
eISSN :
2079-7737
Publisher :
MDPI AG
Volume :
12
Issue :
2
Pages :
234
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
CSUB - California State University, Bakersfield [US-CA] [US-CA]
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
Funding number :
Louis Stokes Alliance for Minority Participation
Funding text :
This research was funded by the CSUB Research Council of the University (RCU), CSUB Faculty Teaching and Learning Center (FTLC), and the Louis Stokes Alliance for Minority Participation (LSAMP-NSF). The work of S.R. and B.D was supported by an Aspirant grants from the FNRS (grant 1.A618.18) and a FRIA grant from the FNRS for S.R. and N.S. (FRIA 1.E.116.21). Transcriptomic analysis was supported by a ‘Crédit De Recherche FNRS grant’ (grant R.FNRS.5240 J.0158.21-CDR).
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