LC-UV as tool for nanovectorized anticancer peptide quality control: evaluation of encapsulation efficiency

Many peptides have today a recognized and growing therapeutic interest. Unlike
conventional small molecules, peptides present a wide range of challenges related to
physicochemical stability during the formulation or analytical development. Furthermore,
many compounds of this emerging class of therapeutics have their targets at the
intracellular level hence, they must be incorporated into suitable carriers to achieve their
therapeutic success. This general concept raises the need to develop analytical tools
enabling the determination of the encapsulation efficiency of LB19, a peptide inhibitor of
LDHB proposed as a new therapeutic approach for cancer treatment, into pH sensitive
liposomes and quantitatively demonstrate the ability of these nanocarriers to achieve
intracellular delivery of their payload in-vitro.