Contribution of Capillary Zone Electrophoresis Hyphenated with Drift Tube Ion Mobility Mass Spectrometry as a Complementary Tool to Microfluidic Reversed Phase Liquid Chromatography for Antigen Discovery.
Gou, Marie-Jia; Köse, Murat Cem; Crommen, Jacqueset al.
2022 • In International Journal of Molecular Sciences, 23 (21), p. 13350
Humans; Chromatography, Reverse-Phase; Tandem Mass Spectrometry/methods; Microfluidics; Cell Line, Tumor; Multiple Myeloma; Electrophoresis, Capillary/methods; capillary zone electrophoresis mass spectrometry; drift tube ion mobility spectrometry; orthogonality; untargeted proteomics
Abstract :
[en] The discovery of new antigens specific to multiple myeloma that could be targeted by novel immunotherapeutic approaches is currently of great interest. To this end, it is important to increase the number of proteins identified in the sample by combining different separation strategies. A capillary zone electrophoresis (CZE) method, coupled with drift tube ion mobility (DTIMS) and quadrupole time-of-flight mass spectrometry (QTOF), was developed for antigen discovery using the human myeloma cell line LP-1. This method was first optimized to obtain a maximum number of identifications. Then, its performance in terms of uniqueness of identifications was compared to data acquired by a microfluidic reverse phase liquid chromatography (RPLC) method. The orthogonality of these two approaches and the physicochemical properties of the entities identified by CZE and RPLC were evaluated. In addition, the contribution of DTIMS to CZE was investigated in terms of orthogonality as well as the ability to provide unique information. In conclusion, we believe that the combination of CZE-DTIMS-QTOF and microfluidic RPLC provides unique information in the context of antigen discovery.
Disciplines :
Hematology
Author, co-author :
Gou, Marie-Jia ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments ; Laboratory for the Analysis of Medicines (LAM), Department of Pharmacy, CIRM,
Crommen, Jacques ; Université de Liège - ULiège > Département de pharmacie ; Laboratory for the Analysis of Medicines (LAM), Department of Pharmacy, CIRM,
Nix, Cindy ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments ; Laboratory for the Analysis of Medicines (LAM), Department of Pharmacy, CIRM,
Cobraiville, Gaël ; Centre Hospitalier Universitaire de Liège - CHU > > Service de rhumatologie ; Laboratory for the Analysis of Medicines (LAM), Department of Pharmacy, CIRM,
Caers, Jo ; Centre Hospitalier Universitaire de Liège - CHU > > Service d'hématologie clinique
Fillet, Marianne ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments ; Laboratory for the Analysis of Medicines (LAM), Department of Pharmacy, CIRM
Language :
English
Title :
Contribution of Capillary Zone Electrophoresis Hyphenated with Drift Tube Ion Mobility Mass Spectrometry as a Complementary Tool to Microfluidic Reversed Phase Liquid Chromatography for Antigen Discovery.
Publication date :
01 November 2022
Journal title :
International Journal of Molecular Sciences
ISSN :
1661-6596
eISSN :
1422-0067
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI), Ch
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