Article (Scientific journals)
Characterization of Myeloperoxidase in the Healthy Equine Endometrium
Parrilla Hernandez, Sonia; Franck, Thierry; Munaut, Carine et al.
2023In Animals, 13 (3), p. 375
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Keywords :
General Veterinary; Animal Science and Zoology
Abstract :
[en] Myeloperoxidase (MPO), as a marker of neutrophil activation, has been associated with equine endometritis. However, in absence of inflammation, MPO is constantly detected in the uterine lumen of estrous mares. The aim of this study was to characterize MPO in the uterus of mares under physiological conditions as a first step to better understand the role of this enzyme in equine reproduction. Total and active MPO concentrations were determined, by ELISA and SIEFED assay, respectively, in low-volume lavages from mares in estrus (n = 26), diestrus (n = 18) and anestrus (n = 8) in absence of endometritis. Immunohistochemical analysis was performed on 21 endometrial biopsies randomly selected: estrus (n = 11), diestrus (n = 6) and anestrus (n = 4). MPO, although mostly enzymatically inactive, was present in highly variable concentrations in uterine lavages in all studied phases, with elevated concentrations in estrus and anestrus, while in diestrus, concentrations were much lower. Intracytoplasmic immunoexpression of MPO was detected in the endometrial epithelial cells, neutrophils and glandular secretions. Maximal expression was observed during estrus in mid and basal glands with a predominant intracytoplasmic apical reinforcement. In diestrus, immunopositive glands were sporadic. In anestrus, only the luminal epithelium showed residual MPO immunostaining. These results confirm a constant presence of MPO in the uterine lumen of mares in absence of inflammation, probably as part of the uterine mucosal immune system, and suggest that endometrial cells are a source of uterine MPO under physiological cyclic conditions.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Parrilla Hernandez, Sonia  ;  Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH)
Franck, Thierry  ;  Université de Liège - ULiège > Centres généraux > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Munaut, Carine  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques
Feyereisen, Emilie ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire
Piret, Joëlle ;  Université de Liège - ULiège > Département de morphologie et pathologie (DMP)
Farnir, Frédéric  ;  Université de Liège - ULiège > Département de gestion vétérinaire des Ressources Animales (DRA)
Reigner, Fabrice;  UE1297 PAO, INRAE, 37380 Nouzilly, France
Barrière, Philippe;  UE1297 PAO, INRAE, 37380 Nouzilly, France
Deleuze, Stefan  ;  Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée
Language :
English
Title :
Characterization of Myeloperoxidase in the Healthy Equine Endometrium
Publication date :
21 January 2023
Journal title :
Animals
eISSN :
2076-2615
Publisher :
MDPI AG
Volume :
13
Issue :
3
Pages :
375
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
ULiège FSR - Université de Liège. Fonds spécial pour la recherche [BE]
Available on ORBi :
since 30 January 2023

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