Identification of staphylococcal cassette chromosome mec in Staphylococcus aureus and non-aureus staphylococci from dairy cattle in Belgium: Comparison of multiplex PCR and whole genome sequencing
[en] The present study compared multiplex PCR (mPCR) and Whole Genome Sequencing (WGS) using the SCCmecFinder database to identify the Staphylococcal Cassette Chromosome (SCC) mec in five Staphylococcus aureus (SA)
and nine non-aureus staphylococci (NAS) isolated from dairy cattle. mPCR identified an SCCmecIV in four SA and
one NAS, but could not differentiate between SCCmecII and IV in the fifth SA, that all harbored the mecA gene
and were phenotypically resistant to cefoxitin. SCCmecFinder confirmed the presence of an SCCmecIVc(2B) in
four SA and of the SCCmecIVa(2B) in the fifth SA and the one NAS. Both methods also detected one untypeable
SCCmec in another cefoxitin-resistant NAS harboring the mecA gene and a pseudo SCCmec in one cefoxitinsensitive NAS harboring one mecC-related gene. No SCCmec elements were identified either in one cefoxitinsensitive NAS harboring the mecA2 gene, or in five NAS (one resistant and four sensitive to cefoxitin)
harboring the mecA1 gene. SCCmecFinder could even not identify the presence of any mecA1 gene in these five
NAS, whose presence was nevertheless confirmed by ResFinder. The conclusions of this study are: (i) mPCR and
WGS sequencing using SCCmecFinder are complementary methodologies to identify SCCmec; (ii) SCCmecFinder
and ResFinder to a lesser extent cannot identify all mec gene allotypes; (iii) a specific classification of the SCCmec
in NAS would be epidemiologically helpful; (iv) presence of a mecA gene and a complete SCCmec is linked to
cefoxitin resistance, whereas presence of other mec genes and of pseudo or no SCCmec is not.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Ngassam Tchamba, Cyrille
Touzain, Fabrice
Fergestad, Marte
De Visscher, Anneleen
L'Abee-Lund, Trine
De Vliegher, Sarne
Wasteson, Yngvild
Blanchard, Yannick
Argudín, Maria A.
Mainil, Jacques ✱; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI)
Thiry, Damien ✱; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Bactériologie vétérinaire et maladies bactériennes animales
✱ These authors have contributed equally to this work.
Language :
English
Title :
Identification of staphylococcal cassette chromosome mec in Staphylococcus aureus and non-aureus staphylococci from dairy cattle in Belgium: Comparison of multiplex PCR and whole genome sequencing
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