Article (Scientific journals)
Differential proliferation and metabolic activity of Sertoli cells in the testes of broiler and layer breeder chickens.
Faure, Mélanie; Guibert, Edith; Crochet, Sabine et al.
2017In Poultry Science, 96 (7), p. 2459-2470
Peer Reviewed verified by ORBi
 

Files


Full Text
1-s2.0-S0032579119314415-main.pdf
Author postprint (1.99 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Animals; Cell Proliferation; Chickens/genetics/growth & development/physiology; Male; Selection, Genetic; Sertoli Cells/physiology; Testis/growth & development; Sertoli cell; chicken; metabolism; testis
Abstract :
[en] Decades of genetic selection have generated 2 different, highly specialized types of chickens in which 1 type, known as the layer-type chicken, expresses high laying performance while the other type, known as the broiler-type chicken, is dedicated to the production of fast-growing birds. Selected lines for the latter type often express disorders in their reproductive performance including early sexual maturation and accelerated, non-reversible seasonal decline of their semen production and mating behavior. The aim of the present study was to characterize some metabolic markers of the Sertoli cell populations. Sertoli cells are somatic cells known to support, coordinate, nourish, and protect the germ cell populations from onset to the end of their meiotic process. Comparisons of gonadal development between males of the 2 genetic types taken at their pre-pubertal period indicated that the testes of layer-type chickens are significantly less developed than in broiler-type males taken at the same age. In addition, cultures of purified Sertoli cells from the 2 types revealed in vitro a higher proliferative capacity when issued from layer compared to broiler-type chickens. This was associated with a higher expression of the genes involved in the beta-oxidation of fatty acids (CPT1; PPARβ) as well as a 4-fold increase in the Lactate Dehydrogenase-A expression and activity. In contrast, Sertoli cells from broiler-type chickens presented an elevated activity of citrate synthase and mitochondria, suggesting a better efficacy of aerobic metabolism in Sertoli cells from broiler compared to layer-type chickens. Moreover, the testis from broiler-type chickens seems to be more sensitive to oxidative stress due to the lower global antioxidant capacity compared to layer-type chickens.In conclusion, these results suggest that the metabolic activity of testicular tissues is different in the layer and broiler breeder chickens. The aerobic metabolism more prevalent in broiler-type chickens could be a factor to reduce the male fertility such as germ cell quality.
Disciplines :
Animal production & animal husbandry
Author, co-author :
Faure, Mélanie  ;  Université de Liège - ULiège > GIGA > GIGA Neurosciences - Neuroendocrinology ; PRC, INRA, CNRS, IFCE, Université de Tours, 37380 Nouzilly, France.
Guibert, Edith;  PRC, INRA, CNRS, IFCE, Université de Tours, 37380 Nouzilly, France.
Crochet, Sabine;  URA, INRA 37380 Nouzilly, France.
Chartrin, Pascal;  URA, INRA 37380 Nouzilly, France.
Brillard, Jean-Pierre;  PRC, INRA, CNRS, IFCE, Université de Tours, 37380 Nouzilly, France.
Collin, Anne;  URA, INRA 37380 Nouzilly, France.
Froment, Pascal;  PRC, INRA, CNRS, IFCE, Université de Tours, 37380 Nouzilly, France.
Language :
English
Title :
Differential proliferation and metabolic activity of Sertoli cells in the testes of broiler and layer breeder chickens.
Publication date :
01 July 2017
Journal title :
Poultry Science
ISSN :
0032-5791
eISSN :
1525-3171
Volume :
96
Issue :
7
Pages :
2459-2470
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
© 2017 Poultry Science Association Inc.
Available on ORBi :
since 19 January 2023

Statistics


Number of views
26 (0 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
11
Scopus citations®
without self-citations
10
OpenCitations
 
10
OpenAlex citations
 
12

Bibliography


Similar publications



Contact ORBi