COVID-19; Diagnosis; ELISA; LFA; SARS-CoV-2; Serology; Antibodies, Viral; Nucleocapsid Proteins; Antibodies, Viral/blood; Betacoronavirus/immunology; COVID-19 Testing; Clinical Laboratory Techniques/methods; Common Cold/diagnosis; Common Cold/virology; Coronavirus Infections/diagnosis; Enzyme-Linked Immunosorbent Assay/methods; Female; Humans; Immunoassay/methods; Male; Mass Screening/methods; Nucleocapsid Proteins/immunology; Pandemics; Pneumonia, Viral/diagnosis; Sensitivity and Specificity; Point-of-Care Testing; Betacoronavirus; Clinical Laboratory Techniques; Common Cold; Coronavirus Infections; Enzyme-Linked Immunosorbent Assay; Immunoassay; Mass Screening; Pneumonia, Viral; Microbiology (medical); Infectious Diseases; General Medicine
Abstract :
[en] Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has infected more than 8 million people worldwide, becoming a pandemic. Detecting antibodies against SARS-CoV-2 is of utmost importance and a good indicator of exposure and circulation of the virus within the general population. Two serological tools based on a double recognition assay [enzyme-linked immunosorbent assay (DR-ELISA) and lateral flow assay (DR-LFA)] to detect total antibodies to SARS-CoV-2 have been developed based on the recombinant nucleocapsid protein. A total of 1065 serum samples, including positive for COVID-19 and negative samples from healthy donors or infected with other respiratory pathogens, were analyzed. The results showed values of sensitivity between 91.2% and 100%, and specificity of 100% and 98.2% for DR-LFA and DR-ELISA, respectively. No cross-reactivity against seasonal coronavirus (HCoV-NL63, HCoV-229E, HCoV-HKU1, HCoV-OC43) was found. These results demonstrate the importance of serology as a complementary tool to polymerase chain reaction for follow-up of recovered patients and identification of asymptomatic individuals.
Disciplines :
Microbiology
Author, co-author :
Hoste, Alexis ; Université de Liège - ULiège > Département GxABT > Microbial technologies ; Eurofins-Inmunología y Genética Aplicada (Eurofins-INGENASA), Madrid, Spain, School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom
Venteo, Angel; Eurofins-Inmunología y Genética Aplicada (Eurofins-INGENASA), Madrid, Spain
Fresco-Taboada, Alba; Eurofins-Inmunología y Genética Aplicada (Eurofins-INGENASA), Madrid, Spain
Tapia, Istar; Eurofins-Inmunología y Genética Aplicada (Eurofins-INGENASA), Madrid, Spain
Monedero, Alejandro; Eurofins-Inmunología y Genética Aplicada (Eurofins-INGENASA), Madrid, Spain
López, Lissette; Eurofins-Inmunología y Genética Aplicada (Eurofins-INGENASA), Madrid, Spain
Jebbink, Maarten F; Laboratory of Experimental Virology, Department of Medical Microbiology, Amsterdam UMC location AMC, University of Amsterdam, Amsterdam, the Netherlands
Pérez-Ramírez, Elisa; Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria-Centro de Investigación en Sanidad Animal (INIA-CISA), Valdeolmos, Madrid, Spain
Jimenez-Clavero, Miguel Angel; Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria-Centro de Investigación en Sanidad Animal (INIA-CISA), Valdeolmos, Madrid, Spain
Almonacid, Mercedes; Madrid Salud-Ayuntamiento de Madrid, Madrid, Spain
Vela, Carmen; Eurofins-Inmunología y Genética Aplicada (Eurofins-INGENASA), Madrid, Spain
van der Hoek, Lia; Laboratory of Experimental Virology, Department of Medical Microbiology, Amsterdam UMC location AMC, University of Amsterdam, Amsterdam, the Netherlands
Rueda, Paloma; Eurofins-Inmunología y Genética Aplicada (Eurofins-INGENASA), Madrid, Spain
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