[en] This study aims to characterise the seeds of two varieties (Beldia and Critical) of hemp grown in four geographical areas of northern Morocco regarding their phenolic compounds and antioxidant power. Thirty-three phenolic compounds were identified using the HPLC-DAD/ESI-MS2 technique. The phenolic profiles of hemp seeds of all varieties from all regions were characterised by their richness in N-trans-caffeoyltyramine (390.22–721.41 μg g−1), cannabisin A (217.96–393.37 μg CTE g−1) and cannabisin B (195.25–331.28 μg CTE g−1). The antioxidant activities (expressed as IC50 and EC50 values) were 1.83–4.14, 1.64–4.37, 2.45–6.02, 2.65–9.29 and 1.75–4.37 mg mL−1 of extract for the TAC, DPPH, ABTS, CUPRAC and FRAP tests respectively. A two-way analysis of variance showed that phenolic compounds' content was mainly related to the geographical location and its interaction with the genotype factor. Multivariate analysis showed that hemp seeds from the Jebha and Galaz regions were characterised by a high level of phenolic compounds and a potent antioxidant activity compared to Tamorot and Ratba regions. This characterisation constitutes an interesting database for breeders to create new varieties that meet fluctuating expectations of the cannabis industry.
Disciplines :
Chemistry Agriculture & agronomy
Author, co-author :
Benkirane, Chaymae ; Laboratory of Agricultural Production Improvement, Biotechnology and Environment, Faculty of Sciences Mohammed I University B.P. 717 Oujda 60000 Morocco
Mansouri, Farid ; Laboratory of Agricultural Production Improvement, Biotechnology and Environment, Faculty of Sciences Mohammed I University B.P. 717 Oujda 60000 Morocco ; Higher School of Education and Training Mohammed I University B.P. 410 Oujda 60000 Morocco
Ben Moumen, Abdessamad; Laboratory of Agricultural Production Improvement, Biotechnology and Environment, Faculty of Sciences Mohammed I University B.P. 717 Oujda 60000 Morocco
Taaifi, Yassine; Laboratory of Agricultural Production Improvement, Biotechnology and Environment, Faculty of Sciences Mohammed I University B.P. 717 Oujda 60000 Morocco
Melhaoui, Reda; Laboratory of Agricultural Production Improvement, Biotechnology and Environment, Faculty of Sciences Mohammed I University B.P. 717 Oujda 60000 Morocco
Caid, Hana Serghini ; Laboratory of Agricultural Production Improvement, Biotechnology and Environment, Faculty of Sciences Mohammed I University B.P. 717 Oujda 60000 Morocco
Fauconnier, Marie-Laure ; Université de Liège - ULiège > TERRA Research Centre > Chimie des agro-biosystèmes
Elamrani, Ahmed; Laboratory of Agricultural Production Improvement, Biotechnology and Environment, Faculty of Sciences Mohammed I University B.P. 717 Oujda 60000 Morocco
Abid, Malika; Laboratory of Agricultural Production Improvement, Biotechnology and Environment, Faculty of Sciences Mohammed I University B.P. 717 Oujda 60000 Morocco
Language :
English
Title :
Phenolic profiles of non‐industrial hemp ( <i>Cannabis sativa</i> L.) seed varieties collected from four different Moroccan regions
Publication date :
2023
Journal title :
International Journal of Food Science and Technology
Moroccan Ministry of Higher Education, , Scientific Research and innovation and the National Agency of Medicinal and Aromatic Plant WBI - Wallonie-Bruxelles International
Alonso-Esteban, J.I., Pinela, J., Ćirić, A. et al. (2022). Chemical composition and biological activities of whole and dehulled hemp (Cannabis sativa L.) seeds. Food Chemistry, 374, 1–9.
Bassolino, L., Buti, M., Fulvio, F. et al. (2020). In silico identification of myb and bhlh families reveals candidate transcription factors for secondary metabolic pathways in Cannabis sativa L. Plants, 9, 1–19.
Benkirane, C., Moumen, A.B., Fauconnier, M.-L. et al. (2022). Bioactive compounds from hemp (Cannabis sativa L.) seeds: optimization of phenolic antioxidant extraction using simplex lattice mixture design and HPLC-DAD/ESI-MS2 analysis. RSC Advances, 12, 25764–25777.
Bibi, N., Shah, M.H., Khan, N. et al. (2022). Variations in total phenolic, total flavonoid contents, and free radicals' scavenging potential of onion varieties planted under diverse environmental conditions. Plants, 11, 950.
Cattaneo, C., Givonetti, A., Leoni, V. et al. (2021). Biochemical aspects of seeds from Cannabis sativa L. plants grown in a mountain environment. Scientific Reports, 11, 3927.
Chen, T., He, J., Zhang, J. et al. (2012). The isolation and identification of two compounds with predominant radical scavenging activity in hempseed (seed of Cannabis sativa L.). Food Chemistry, 134, 1030–1037.
Chouvy, P.A. & Afsahi, K. (2014). Hashish revival in Morocco. International Journal of Drug Policy, 25, 416–423.
Chrysargyris, A., Evangelides, E. & Tzortzakis, N. (2021). Seasonal variation of antioxidant capacity, phenols, minerals and essential oil components of sage, spearmint and Sideritis plants grown at different altitudes. Agronomy, 11, 1766.
Crini, G., Lichtfouse, E., Chanet, G. & Morin-Crini, N. (2020). Applications of hemp in textiles, paper industry, insulation and building materials, horticulture, animal nutrition, food and beverages, nutraceuticals, cosmetics and hygiene, medicine, agrochemistry, energy production and environment: a review. Environmental Chemistry Letters, 18, 1451–1476.
Farinon, B., Molinari, R., Costantini, L. & Merendino, N. (2020). The seed of industrial hemp (Cannabis sativa L.): nutritional quality and potential functionality for human health and nutrition. Nutrients, 12, 1935.
Faugno, S., Piccolella, S., Sannino, M. et al. (2019). Can agronomic practices and cold-pressing extraction parameters affect phenols and polyphenols content in hempseed oils? Industrial Crops and Products, 130, 511–519.
Flores-Sanchez, I.J. & Verpoorte, R. (2008). Secondary metabolism in cannabis. Phytochemistry Reviews, 7, 615–639.
Frassinetti, S., Moccia, E., Caltavuturo, L. et al. (2018). Nutraceutical potential of hemp (Cannabis sativa L.) seeds and sprouts. Food Chemistry, 262, 56–66.
Glivar, T., Er, J., Kreft, S. et al. (2020). Cannabinoid content in industrial hemp (Cannabis sativa L.) varieties grown in Slovenia. Industrial Crops and Products, 145, 112028.
Grochowski, D.M., Uysal, S., Aktumsek, A. et al. (2017). In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca. Phytochemistry Letters, 20, 365–372.
Huang, W., Cai, Y., Zhang, Y., Huang, W. & Cai, Y. (2009). Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention. Nutrition and Cancer, 62, 1–20.
Irakli, M., Tsaliki, E., Kalivas, A., Kleisiaris, F., Sarrou, E. & Cook, C.M. (2019). Effect of genotype and growing year on the nutritional, phytochemical, and antioxidant properties of industrial hemp (Cannabis sativa L.) seeds. Antioxidants, 8, 491.
Isidore, E., Karim, H. & Ioannou, I. (2021). Extraction of phenolic compounds and terpenes from Cannabis sativa L. by-products: from conventional to intensified processes. Antioxidants, 10, 942.
Izzo, L., Castaldo, L., Narváez, A. et al. (2020). Analysis of phenolic compounds in commercial Cannabis sativa L. inflorescences using UHPLC-Q-orbitrap HRMS. Molecules, 25, 631.
Kadriye, I., Demirata, B. & Apak, R. (2012). Determination of total antioxidant capacity of lipophilic and hydrophilic antioxidants in the same solution by using ferric – ferricyanide assay. Food Analytical Methods, 5, 1150–1158.
Leonard, W., Zhang, P., Ying, D. & Fang, Z. (2020a). Hempseed in food industry: nutritional value, health benefits, and industrial applications. Comprehensive Reviews in Food Science and Food Safety, 19, 282–308.
Leonard, W., Zhang, P., Ying, D. & Fang, Z. (2020b). Lignanamides: sources, biosynthesis and potential health benefits–a minireview. Critical Reviews in Food Science and Nutrition, 61, 1404–1414.
Leonard, W., Zhang, P., Ying, D., Xiong, Y. & Fang, Z. (2021). Extrusion improves the phenolic profile and biological activities of hempseed (Cannabis sativa L.) hull. Food Chemistry, 346, 128606.
Mansouri, F., Moumen, A.B., Belhaj, K. et al. (2018). Effect of crop season on the quality and composition of extra virgin olive oils from Greek and Spanish varieties grown in the oriental region of Morocco. Emirates Journal of Food and Agriculture, 30, 549–562.
Menga, V., Garofalo, C., Suriano, S. et al. (2022). Phenolic acid composition and antioxidant activity of whole and defatted seeds of Italian hemp cultivars: a two-year case study. Agriculture (Switzerland), 12, 759.
Moccia, S., Siano, F., Russo, G.L. et al. (2020). Antiproliferative and antioxidant effect of polar hemp extracts (Cannabis sativa L., fedora cv.) in human colorectal cell lines. International Journal of Food Sciences and Nutrition, 71, 410–423.
Mutha, R.E., Tatiya, A.U. & Surana, S.J. (2021). Flavonoids as natural phenolic compounds and their role in therapeutics: an overview. Future Journal of Pharmaceutical Sciences, 7, 25.
Nigro, E., Crescente, G., Formato, M. et al. (2020). Hempseed lignanamides rich-fraction: chemical investigation and cytotoxicity towards U-87 glioblastoma cells. Molecules, 25, 1–25.
Ning, K., Hou, C., Wei, X. et al. (2022). Metabolomics analysis revealed the characteristic metabolites of hemp seeds varieties and metabolites responsible for antioxidant properties. Frontiers in Plant Science, 13, 904163.
Pojić, M., Mišan, A., Sakač, M. et al. (2014). Characterization of byproducts originating from hemp oil processing. Journal of Agricultural and Food Chemistry, 62, 12436–12442.
Pollastro, F., Minassi, A. & Fresu, L.G. (2018). Cannabis phenolics and their bioactivities. Current Medicinal Chemistry, 25, 1160–1185.
Rea Martinez, J., Montserrat-De La Paz, S., De La Puerta, R., García-Giménez, M.D. & Fernández-Arche, M.Á. (2020). Characterization of bioactive compounds in defatted hempseed (Cannabis sativa L.) by UHPLC-HRMS/MS and anti-inflammatory activity in primary human monocytes. Food and Function, 11, 4057–4066.
Roumani, M., Duval, R.E., Ropars, A., Risler, A., Robin, C. & Larbat, R. (2020). Phenolamides: plant specialized metabolites with a wide range of promising pharmacological and health-promoting interests. Biomedicine and Pharmacotherapy, 131, 110762.
Schluttenhofer, C. & Yuan, L. (2017). Challenges towards revitalizing hemp: a multifaceted crop. Trends in Plant Science, 22, 917–929.
Taaifi, Y., Benmoumen, A., Belhaj, K. et al. (2021). Seed composition of non-industrial hemp (Cannabis sativa L.) varieties from four regions in northern Morocco. International Journal of Food Science & Technology, 56, 5931–5947.
Zhou, Y., Wang, S., Ji, J., Lou, H. & Fan, P. (2018). Hemp (Cannabis sativa L.) seed Phenylpropionamides composition and effects on memory dysfunction and biomarkers of neuroinflammation induced by lipopolysaccharide in mice. ACS Omega, 3, 15988–15995.