Relationship of foetal number and parity in Barbari goats to plasma profile of caprine pregnancy-associated glycoprotein (caPAG) during gestation and the early postpartum period.

Singh, S P; Ramachandran, N; Sharma, N; Goel, A K; de Sousa, N M; Beckers, Jean-François; Swain, D K; Singh, M K; Kharche, S D

doi:10.1016/j.anireprosci.2019.106190

Article (Scientific journals)

Relationship of foetal number and parity in Barbari goats to plasma profile of caprine pregnancy-associated glycoprotein (caPAG) during gestation and the early postpartum period.

Singh, S P; Ramachandran, N; Sharma, N et al.

2019 • In Animal Reproduction Science, 210, p. 106190

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/298225

DOI
10.1016/j.anireprosci.2019.106190

PubMed
31635784

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Keywords :

Caprine pregnancy-associated glycoprotein; ELISA; Gestation; Goats; Postpartum; Pregnancy Proteins; Animals; Female; Gene Expression Regulation/physiology; Goats/blood; Goats/physiology; Parity; Postpartum Period/blood; Pregnancy; Pregnancy Proteins/blood; Pregnancy Proteins/genetics; Pregnancy Proteins/metabolism; Litter Size; Pregnancy, Animal/physiology; Gene Expression Regulation; Postpartum Period; Pregnancy, Animal; Food Animals; Animal Science and Zoology; Endocrinology; General Medicine

Abstract :

[en] This study was conducted to characterise pregnancy-associated glycoprotein (caPAG) in peripheral plasma during gestation and postpartum periods of nulliparous and multiparous does with one or two foetuses using a caPAG specific two-step sandwich ELISA system. Earliest time-points for detection of pregnancy and foetal number with appropriate cut-off values were identified. Plasma samples from 15 pregnant (multiparous: n = 8; nulliparous: n = 7; during pregnancy and postpartum period) and six non-pregnant (during oestrous cycle) goats were collected and analysed. Mean caPAG concentration was greater than the threshold for pregnancy detection (S-N = 0.40) on d22, peaked on d45 and remained unchanged until parturition. From d45 until parturition, caPAG concentration in multiparous does with two foetuses was 1.4 to 1.8 fold greater (P < 0.001) than those with one foetus. For the ELISA, 0.83 (S-N) was the most appropriate cut-off to differentiate does with two from those with a single foetus with an overall sensitivity and accuracy of 88.9% and 84.7%, respectively. Circulating caPAG concentration in multiparous goats was greater (P < 0.05) compared with nulliparous goats during the early pregnancy and postpartum periods. After parturition, caPAG concentrations markedly decreased and were basal within 14 days postpartum. In conclusion, using the caPAG specific ELISA, results indicated there were unique gestational and postpartum profiles for caPAG concentrations that are affected by number of foetuses and parity of the doe. The marked decrease in concentration of caPAG following parturition indicates there would not be compromising of the detection of subsequent pregnancies in goats using this technique.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Singh, S P; Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, India. Electronic address: shiva.singh@icar.gov.in

Ramachandran, N; Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, India

Sharma, N; Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, India

Goel, A K; Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, India

de Sousa, N M; Laboratory of Animal Endocrinology and Reproduction, Faculty of Veterinary Medicine, University of Liege, 4000, Belgium

Beckers, Jean-François ; Université de Liège - ULiège > Département des sciences fonctionnelles (DSF)

Swain, D K; Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, Uttar Pradesh, India

Singh, M K; Animal Genetics and Breeding Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, India

Kharche, S D; Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, India

Language :

English

Title :

Relationship of foetal number and parity in Barbari goats to plasma profile of caprine pregnancy-associated glycoprotein (caPAG) during gestation and the early postpartum period.

Publication date :

November 2019

Journal title :

Animal Reproduction Science

ISSN :

0378-4320

eISSN :

1873-2232

Publisher :

Elsevier B.V., Netherlands

Volume :

210

Pages :

106190

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://api.elsevier.com/content/article/PII:S0378432019303331?httpAccept=text/xml

Funders :

SERB - Science and Engineering Research Board

Funding text :

The study was supported by a grant from Science and Engineering Research Board-Department of Science and Technology (SERB-DST), Government of India, New Delhi (Grant No.: YSS/2015/001518 ). The authors are thankful for the support extended by the Director of the Institute for providing necessary facilities to carry out this study. The skilled technical assistance by Mr Dinesh Bhatt and Mr H. A. Himkar and help from Dr Deepak Dwivedi and Mr Ranjeet Singh are duly acknowledged.

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