[en] ("[en] PURPOSE: To describe bacterial isolates and associated antibiotic resistance from horses with ulcerative keratitis in Belgium.
METHODS: Medical records from horses with ulcerative keratitis presented to the ophthalmology service of the Veterinary teaching hospital of Liege, Belgium, between 2014 and 2021 were evaluated. Bacterial isolates were identified and VITEK® 2 (Biomérieux) provided antimicrobial susceptibility testing and resistance detection.
RESULTS: Two hundred eyes of 196 horses were sampled. Ninety-seven eyes had a positive bacterial culture (48.5%) and 139 bacterial isolates were identified. Staphylococcus (63/139: 45.3%) and Streptococcus (33/139: 23.7%) were the most frequent genus isolated. Staphylococcus aureus (21/139: 15.1%) was the most frequent species isolated of which half were methicillin-resistant (MRS). Streptococcus equi subsp. zooepidemicus (19/139: 13.7%) was the second most identified bacterial isolate. Only two Pseudomonas species were isolated (2/139: 1.4%). The overall resistance of all bacterial isolates against chloramphenicol (12.4%) and fluoroquinolones (14.3%) was low. Resistance against tobramycin, polymyxin B, gentamicin, fusidic acid, tetracycline, and neomycin ranged from 40.8% to 58.6%. When separating the MRS from the other staphylococci, a significant difference was noted in percentage of resistance to gentamicin (p = .00026) and tetracycline (p = .00015). MRS were highly resistant to gentamicin (75%) and tetracycline (100%), whereas the remaining staphylococci were significantly less resistant to gentamicin (17%) and tetracycline (40.4%).
CONCLUSION: Although Pseudomonas species has been rarely cultured, our results are roughly consistent with previous studies. Multiple drug resistance was high and resistance to first-choice antibiotics in ulcerative keratitis was noted. These results warrant continued monitoring of susceptibility profile.","[en] ","")
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Vercruysse, Eline ; Université de Liège - ULiège > Département d'Enseignement et de Clinique des animaux de Compagnie (DCC) > Ophtalmologie des animaux de compagnie
Narinx, Florine ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée
Rives, Albane ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Productions animales durables
Sauvage, Aurélie ; Université de Liège - ULiège > Département d'Enseignement et de Clinique des Equidés (DCE) > Ophtalmologie des équidés
Grauwels, Magda ; Université de Liège - ULiège > Département d'Enseignement et de Clinique des Equidés (DCE) > Ophtalmologie des équidés
Monclin, Sébastien ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée
Language :
English
Title :
Equine ulcerative keratitis in Belgium: Associated bacterial isolates and in vitro antimicrobial resistance in 200 eyes.
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