Faecal excretion; Grazing; Indoor; Italian ryegrass; LIPE®; Pearl millet; Animal Science and Zoology; Veterinary (all); General Veterinary
Abstract :
[en] A series of trials were conducted in different experimental and feeding conditions to evaluate a purified and enriched lignin extracted from Eucalyptus grandis (LIPE®) as a marker to estimate faecal output in sheep fed temperate and tropical grasses. Two indoor trials were conducted in metabolism cages: the first one (Trial I) was conducted with sheep fed Italian ryegrass (Lolium multiflorum Lam.) pastures, which was evaluated in two different periods [Period I (n = 14), and Period II (n = 15)], and the second one (Trial II) was performed with sheep fed pearl millet [Pennisetum americanum (L.) Leeke] pastures (n = 16). Grazing trials were performed with sheep grazing Italian ryegrass [Trial III (n = 62)] and pearl millet [Trial IV (n = 34)]. According to the manufacturer's statements, sheep were dosed once a day with capsules containing 250 mg of LIPE® for a period of seven days, beginning two days before total faecal collections started and finishing on the last faecal collection day. To measure the observed daily faecal output, sheep were harnessed with faecal bags that were emptied once a day at 0800 h for five days. Faecal subsamples from indoor and grazing trials were taken to determine the LIPE® content by infrared spectroscopy. Regression analyses performed between estimated and observed faecal output (g DM/kg LW/day) by sheep were not significant (p > 0.05) in three of the four evaluated trials. Faecal LIPE® recovery from the total data of the four trials showed a statistical difference when compared to the reference value (100%). In conclusion, our results show that the LIPE® marker presents low accuracy to estimate the daily faecal output of sheep fed temperate (Italian ryegrass) and tropical (pearl millet) grasses.
Disciplines :
Animal production & animal husbandry
Author, co-author :
Amaral, Gláucia Azevedo do; Department of Agricultural Diagnosis and Research, Hulha Negra, Brazil
Azevedo, Eduardo Bohrer de ; Department of Animal Science, Federal University of Santa Maria, Santa Maria, Brazil ; Federal University of Pampa, Itaqui, Brazil
Savian, Jean Víctor; Instituto Nacional de Investigación Agropecuaria (INIA). Programa Pasturas y Forrajes. Estación Experimental INIA Treinta y Tres, Treinta y Tres, Uruguay
Bremm, Carolina; Department of Agricultural Diagnosis and Research, Porto Alegre, Brazil ; Grazing Ecology Research Group, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
David, Diego Bitencourt de; Federal University of Pampa, Itaqui, Brazil ; Department of Agricultural Diagnosis and Research, São Gabriel, Brazil
Gonda, Horacio Leandro ; Swedish University of Agricultural Sciences, Uppsala, Sweden
Bindelle, Jérôme ; Université de Liège - ULiège > TERRA Research Centre > Ingénierie des productions animales et nutrition
Carvalho, Paulo César de Faccio; Grazing Ecology Research Group, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
Language :
English
Title :
Purified and enriched lignin as a marker to estimate faecal output of sheep fed temperate and tropical grasses
CAPES - Coordenação de Aperfeicoamento de Pessoal de Nível Superior CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
Funding text :
The authors would like to thank the financial support from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) and National Council for Scientific and Technological Development - Brasil (CNPq).
Ahvenjärvi, S., Nyholm, L., Nousiainen, J., Mäntysaari, E.A., Lidauer, M., Polyethylene glycol as an indigestible marker to estimate fecal output in dairy cows. J. Dairy Sci. 101 (2018), 4245–4258, 10.3168/jds.2017-13337.
Azevedo, E.B., Poli, C.H.E.C., David, D.B., Amaral, G.A., Fonseca, L., Carvalho, P.C.F., Fischer, V., Morris, S.T., Use of faecal components as markers to estimate intake and digestibility of grazing sheep. Livest. Sci. 165 (2014), 42–50, 10.1016/j.livsci.2014.04.018.
Bannink, A., Ellis, J.L., Mach, N., Spek, J.W., Dijkstra, J., Interactions between enteric methane and nitrogen excretion in dairy cows. Animal 4 (2013), 19–27, 10.1017/S2040470013000277.
Baurhoo, B., Ruiz-Feria, C.A., Zhao, X., Purified lignin: nutritional and health impacts on farm animals - a review. Anim. Feed Sci. Technol. 144 (2008), 175–184.
Benvenutti, M.A., Findsen, C., Savian, J.V., Mayer, D.G., David, G., The effect of stage of regrowth on the physical composition and nutritive value of the various vertical strata of kikuyu (Cenchrus clandestinus) pastures. Trop. Grassl. 8:2 (2020), 141–146, 10.17138/TGFT(8)141-146.
Berchielli, T.T., Oliveira, S.G., Carrilho, E.N.V.M., Feitosa, J.V., Lopes, A.D., Comparison of markers used to estimate fecal production and digesta flow of cattle. Rev. Bras. Zootec. 34 (2005), 987–996, 10.1590/S1516-35982005000300032.
Carvalho, P.C.F., Kozloski, G.V., Ribeiro Filho, H.M.N., Reffatti, M.V., Genro, T.C.M., Euclides, V.P.B., Advances in methods for determining animal intake on pasture. Rev. Bras. Zootec. 36 (2007), 151–170, 10.1590/s1516-35982007001000016.
Cochran, R.C., Adams, D.C., Wallace, J.D., Galyean, M.L., Predicting digestibility of different diets with internal markers: evaluation of four potential markers. J. Anim. Sci. 63 (1986), 1476–1483, 10.2527/jas1986.6351476x.
David, D.B., Poli, C.H.E.C., Savian, J.V., Amaral, G.A., Azevedo, E.B., Carvalho, P.C.F., McManus, C.M., Faecal index to estimate intake and digestibility in grazing sheep. J. Agric. Sci. 152 (2014), 667–674, 10.1017/S0021859613000294.
Delagarde, R., Pérez-Ramírez, E., Peyraud, J.L., Ytterbium oxide has the same accuracy as chromic oxide for estimating variations of faecal dry matter output in dairy cows fed a total mixed ration at two feeding levels. Anim. Feed Sci. Technol. 161 (2010), 121–131, 10.1016/j.anifeedsci.2010.08.006.
Detmann, E., Paulino, M.F., Zervoudakis, J.T., Valadares Filho, S.C., Euclydes, R.F., Lana, R.P., Queiroz, D.S., Chromium and internal markers in intake determination by crossbred steers supplemented at pasture. Rev. Bras. Zootec. 30 (2001), 1600–1609, 10.1590/S1516-35982001000600030.
De Vries, M.F.W., Estimating forage intake and quality in grazing cattle: a reconsideration of the hand-plucking method. J. Range Manag. 48 (1995), 370–375, 10.2307/4002491.
Dixon, R.M., Anderson, S.T., Kidd, L.J., Fletcher, M.T., Management of phosphorus nutrition of beef cattle grazing seasonally dry rangelands: a review. Anim. Prod. Sci. 60 (2020), 863–879, 10.1071/AN19344.
Ferret, A., Plaixats, J., Caja, G., Gasa, J., Prió, P., Using markers to estimate apparent dry matter digestibility, faecal output and dry matter intake in dairy ewes fed Italian ryegrass hay or alfalfa hay. Small Rumin. Res. 33 (1999), 145–152, 10.1016/S0921-4488(99)00015-2.
Figueiredo, M.R.P., Saliba, E.O.S., Barbosa, G.S.S.C., Silva, F.A.E., Nunes, A.N., Da Mota E Silva, C.R., Moreira, G.R., Use of indigestible markers to estimate the apparent dry matter digestibility of diets containing a cocoa by-product. Semin. Cienc. Agrar. 40 (2019), 2771–2782, 10.5433/1679-0359.2019v40n6p2771.
Freitas, H.P., Lage, C.F.A., Malacco, V.M.R., Moura, A.M., Rodrigues, J.P.P., Saturnino, H.M., Coelho, S.G., Reis, R.B., Partial substitution of soybean meal with a yeast-derived protein in the diet of dairy cows under a rotational grazing system. Livest. Sci. 225 (2019), 144–150, 10.1016/j.livsci.2019.05.008.
Glindemann, T., Tas, B.M., Wang, C., Alvers, S., Susenbeth, A., Evaluation of titanium dioxide as an inert marker for estimating faecal excretion in grazing sheep. Anim. Feed Sci. Technol. 152 (2009), 186–197, 10.1016/j.anifeedsci.2009.04.010.
Hassoun, P., Viudes, G., Autran, P., Bastianelli, D., Bocquier, F., A method for estimating dry forage intake by sheep using polyethylene glycol as a faecal marker measured with NIRS. Animal 7:8 (2013), 1280–1288, 10.1017/S1751731113000323.
Hristov, A.N., Ahvenjarvi, S., McAllister, T.A., Huhtanen, P., Composition and digestive tract retention time of ruminal particles with functional specific gravity greater or less than 1.02. J. Anim. Sci. 81 (2003), 2639–2648, 10.2527/2003.81102639x.
Judkins, M.B., Krysl, L.J., Barton, R.K., Estimating diet digestibility: a comparison of 11 techniques across six different diets fed to rams. J. Anim. Sci. 68 (1990), 1405–1415, 10.2527/1990.6851405x.
Kozloski, G.V., Oliveira, L., Poli, C.H.E.C., Azevedo, E.B., David, D.B., Ribeiro Filho, H.M.N., Collet, S.G., Faecal nitrogen excretion as an approach to estimate forage intake of wethers. J. Anim. Physiol. Anim. Nutr. 98 (2014), 659–666, 10.1111/jpn.12118.
Lanzetta, V.A.S., Rezende, A.S.C., Saliba, E.O.S., Lana, Â.M.Q., Rodriguez, N.M., Moss, P.C.B., Validation of Lipe® as method to evaluate the apparent digestibility of nutrients in equines. Rev. Bras. Zootec. 38 (2009), 69–74, 10.1590/S1516-35982009000100009.
Lima, J.B.M.P., Graça, D.S., Borges, A.L.C.C., Saliba, E.O.S., Simão, S.M.B., Use of chromic oxide and LIPE® as external markers to estimate dry matter intake by beef calves. Arq. Bras. Med. Vet. Zootec. 60 (2008), 1197–1204, 10.1590/S0102-09352008000500023.
Merchen, N.R., Digestion, absorption and excretion in ruminants. Church, D.C., (eds.) The Ruminant Animal Digestive Physiology and nutrition. O&B Books, Carvallis, 1993, 172–201.
Mioto da Costa, M.C., Ítavo, L.C.V., Ferreira Ítavo, C.C.B., Dias, A.M., Petit, H.V., Reis, F.A., Gomes, R.C., Leal, E.S., Niwa, M.V.G., De Moraes, G.J., Evaluation of internal and external markers to estimate faecal output and feed intake in sheep fed fresh forage. Anim. Prod. Sci. 59 (2019), 741–748, 10.1071/AN16567.
Mott, G.O., Lucas, H.L., The design, conduct, and Interpretation of Grazing Trials on Cultivated and Improved Pastures. 1952, International Grassland Congress, Pennsylvania, 1380–1385.
Owens, F.N., Hanson, C.F., External and internal markers for appraising site and extent of digestion in ruminants. J. Dairy Sci. 75 (1992), 2605–2617, 10.3168/jds.S0022-0302(92)78023-0.
Penning, P.D., Animal-based techniques for estimating herbage intake. Penning, P.D., (eds.) Herbage Intake Handbook, second ed., 2004, British Grassland Society, Reading, UK, 53–93.
Pinheiro, J.C., Bates, D.M., Linear mixed-effects models: basic concepts and examples. Mixed-Effects Models in S and S-PLUS. Statistics and Computing, 2000, Springer, New York, NY, 10.1007/0-387-22747-4_1.
Prigge, E.C., Varga, G.A., Vicini, J.L., Reid, R.L., Comparison of ytterbium chloride and chromium sesquioxide as fecal indicators. J. Anim. Sci. 53 (1981), 1629–1633, 10.2527/jas1982.5361629x.
Robertson, J.B., Van Soest, P.J., The detergent system of analysis and its application to human foods. James, W.P.T., Theander, O., (eds.) The Analysis of Dietary Fiber in Food, 1981, Marcel Dekker, New York, 123–158.
Rymer, C., Givens, D.I., Owen, E., Axford, R.F.E., The measurement of forage digestibility in vivo. Omed, H.M., (eds.) Forage Evaluation in Ruminant Nutrition, 2000, CABI, Wallingford, 113–134.
Sales, J., Janssens, G.P.J., Acid-insoluble ash as a marker in digestibility studies: a review. J. Anim. Feed Sci. 12 (2003), 383–401, 10.22358/jafs/67718/2003.
Saliba, E.O.S., Faria, E.P., Rodriguez, N.M., Moreira, G.R., Machado Sampaio, I.B., Saliba, J.S., Gonçalves, L.C., Borges, I., Cruz Borges, A.L.C., Use of infrared spectroscopy to estimate fecal output with marker Lipe®. Int. J. Food Sci. Nutr. Diet., 2015, 1–10, 10.19070/2326-3350-si04001.
Saliba, E.O.S., Rodriguez, N.M., Piló-Veloso, D., Utilization of purified lignin extracted from Eucalyptus grandis (PELI), used as an external marker in digestibility trials in various animal species. 9° World Conference on Animal Production. Porto Alegre, 2003.
Santana, M.C.A., Fiorentini, G., Messana, J.D., Dian, P.H.M., Canesin, R.C., Reis, R.A., Berchielli, T.T., Different forms and frequencies of soybean oil supplementation do not alter rumen fermentation in grazing heifers. Anim. Prod. Sci. 57 (2017), 530–538, 10.1071/AN14608.
Savian, J.V., Genro, T.C.M., Neto, A.B., Bremm, C., Azevedo, E.B., David, D.B., Gonda, H.L., Carvalho, P.C.F., Comparison of faecal crude protein and n-alkanes techniques to estimate herbage intake by grazing sheep. Anim. Feed Sci. Technol. 242 (2018), 144–149, 10.1016/j.anifeedsci.2018.06.010.
Savian, J.V., Schons, R.M.T., Mezzalira, J.C., Barth Neto, A., Da Silva Neto, G.F., Benvenutti, M.A., Carvalho, P.C.F., A comparison of two rotational stocking strategies on the foraging behaviour and herbage intake by grazing sheep. Animal 14:12 (2020), 2503–2510, 10.1017/S1751731120001251.
Silva, D.K.A., Bezerra Cordeiro, F.S., da Silva, É.C.L., Cardoso, D.B., Rodrigues Magalhães, A.L., Silva de Melo, A.A., Pereira, K.P., dos Santos Silva, E.T., Intake, performance, carcass traits and meat quality of goats grazing in the Caatinga rangeland. Semin. Agrar. 41 (2020), 1639–1652, 10.5433/1679-0359.2020v41n5p1639.
Silva, W.L., Costa, J.P.R., Caputti, G.P., Lage Filho, N.M., Ruggieri, A.C., Reis, R.A., Effects of grazing intensity and supplementation strategies on Tifton 85 production and on sheep performance. Small Rumin. Res. 174 (2019), 118–124, 10.1016/j.smallrumres.2019.03.015.
Smith, A.M., Reid, J.T., Use of chromic oxide as an indicator of fecal output for the purpose of determining the intake of pasture herbage by grazing cows. J. Dairy Sci. 38 (1955), 515–524, 10.3168/jds.S0022-0302(55)95006-2.
Titgemeyer, E.C., Design and interpretation of nutrient digestion studies. J. Anim. Sci. 75 (1997), 2235–2247, 10.2527/1997.7582235x.
Warner, A.C.I., Rate of passage of digesta through the gut of mammals and birds. Nutr. Abstr. Rev. 51 (1981), 789–820.