[en] Apple scab is an important disease conventionally controlled by chemical fungicides, which should be replaced by more environmentally friendly alternatives. One of these alternatives could be the use of lipopeptides produced by Bacillus subtilis. The objective of this work is to study the action of the three families of lipopeptides and different mixtures of them in vitro and in vivo against Venturia inaequalis. Firstly, the antifungal activity of mycosubtilin/surfactin and fengycin/surfactin mixtures was determined in vitro by measuring the median inhibitory concentration. Then, the best lipopeptide mixture ratio was produced using Design of Experiment (DoE) to optimize the composition of the culture medium. Finally, the lipopeptides mixtures efficiency against V. inaequalis was assessed in orchards as well as the evaluation of the persistence of lipopeptides on apple. In vitro tests show that the use of fengycin or mycosubtilin alone is as effective as a mixture, with the 50-50% fengycin/surfactin mixture being the most effective. Optimization of culture medium for the production of fengycin/surfactin mixture shows that the best composition is glycerol coupled with glutamic acid. Finally, lipopeptides showed in vivo antifungal efficiency against V. inaequalis regardless of the mixture used with a 70% reduction in the incidence of scab for both mixtures (fengycin/surfactin or mycosubtilin/surfactin). The reproducibility of the results over the two trial campaigns was significantly better with the mycosubtilin/surfactin mixture. The use of B. subtilis lipopeptides to control this disease is very promising.
Disciplines :
Microbiology
Author, co-author :
Leconte, Aline ; Université de Liège - ULiège > TERRA Research Centre ; UMRt BioEcoAgro 1158-INRAE, Equipe Métabolites Secondaires D'origine Microbienne, Institut Charles Viollette, Université de Lille, F-59000 Lille, France ; JUNIA, UMRt BioEcoAgro 1158-INRAE, Equipe Métabolites Spécialisés D'origine Végétale, Institut Charles Viollette, F-59000 Lille, France
Tournant, Ludovic; FREDON Hauts-de-France-265 rue Becquerel, F-62750 Loos-en-Gohelle, France
Muchembled, Jérôme; JUNIA, UMRt BioEcoAgro 1158-INRAE, Equipe Métabolites Spécialisés D'origine Végétale, Institut Charles Viollette, F-59000 Lille, France
Paucellier, Jonathan; UMRt BioEcoAgro 1158-INRAE, Equipe Métabolites Secondaires D'origine Microbienne, Institut Charles Viollette, Université de Lille, F-59000 Lille, France
Héquet, Arnaud; Lipofabrik SAS, 917 rue des Saules, F-59343 Lesquin, France
Deracinois, Barbara ; UMRt BioEcoAgro 1158-INRAE, Equipe Métabolites Secondaires D'origine Microbienne, Institut Charles Viollette, Université de Lille, F-59000 Lille, France
Deweer, Caroline ; JUNIA, UMRt BioEcoAgro 1158-INRAE, Equipe Métabolites Spécialisés D'origine Végétale, Institut Charles Viollette, F-59000 Lille, France
Krier, François ; UMRt BioEcoAgro 1158-INRAE, Equipe Métabolites Secondaires D'origine Microbienne, Institut Charles Viollette, Université de Lille, F-59000 Lille, France
Deleu, Magali ; Université de Liège - ULiège > TERRA Research Centre > Chimie des agro-biosystèmes
Oste, Sandrine; FREDON Hauts-de-France-265 rue Becquerel, F-62750 Loos-en-Gohelle, France
Jacques, Philippe ; Université de Liège - ULiège > TERRA Research Centre ; Lipofabrik SAS, 917 rue des Saules, F-59343 Lesquin, France
Coutte, François ; UMRt BioEcoAgro 1158-INRAE, Equipe Métabolites Secondaires D'origine Microbienne, Institut Charles Viollette, Université de Lille, F-59000 Lille, France ; Lipofabrik SAS, 917 rue des Saules, F-59343 Lesquin, France
Language :
English
Title :
Assessment of Lipopeptide Mixtures Produced by Bacillus subtilis as Biocontrol Products against Apple Scab (Venturia inaequalis).
ERDF - European Regional Development Fund SME Instrument H2020
Funding text :
These studies were carried out with the financial support of the ERDF (European Regional Development Fund) and the Hauts-de-France Region, as part of the Smartbiocontrol programme (BioProd and BioProtect) included in the INTERREG V France–Walloon–Vlaanderen programme, the ALIBIOTECH programme and the AgriBioPOM project financed by the call for projects on agricultural systems and the environment in the Hauts-de-France Region and for Lipofabrik in 2019 and 2020 by the grant agreement of the SME instrument no. 849713 under the European Union’s Horizon 2020 research and innovation programme.The authors would like to thank students from Polytech-lille (Sarah Berrada, Alexis Delrue, Nadine Halabi, Sarah Klai, Julia Morieux, Raphaël Leroux, Margot Thiblet et Mélissa Legrand) for their participation in the optimization Landy medium for the production of lipopeptides by B. subtilis ATCC 21332 and for the validation of QuEChERS method, as well as Emeline Bertrand from Institut Charles Viollette and Thomas Delhez from University of Liège for their help in lipopeptide production. DoE and mass spectrometry analysis were and performed on the REALCAT platform, funded by a French governmental subsidy managed by the French National Research Agency (ANR) within the framework of the “Future Investments’ program (ANR-11- EQPX-0037). The Hauts-de-France region, FEDER, Ecole Centrale de Lille, and Centrale Initiatives Foundation are also warmly acknowledged for their financial contributions to the acquisition of the REALCAT platform equipment.
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