Faba bean; Gene expression; In silico analysis; Osmotic stress; qRT-PCR; Salt stress; Physiology; Agronomy and Crop Science; Plant Science
Abstract :
[en] Drought and salinity are the most important environmental constraints affecting faba bean (Vicia faba L.) development and crop yield in Tunisia and other Mediterranean countries. Through using different strategies, associating in silico analysis of gene expression and qRT-PCR, this study aims at identifying key genes of faba bean molecular pathways potentially involved in salt and drought response. The impact of these stresses on several physiological and biochemical parameters were investigated in two genotypes (Bachar and Giza 3). To uncover abiotic stress-related genes and better understand the mechanisms of salt and drought stress tolerance in faba bean, a total of 25 faba bean genes were identified through in silico analysis. These genes were associated with important cellular processes such as transcription regulation, signal transport, kinases, phytohormonal signaling, and defense/stress responses. Most of the studied candidates were expressed at various levels in different organs including leaves, roots, flowers, stems, cotyledons, and seeds suggesting a potential role in the growth and development of faba bean plants. Furthermore, qRT-PCR was used to study gene expression profiles in leaves and roots of Bachar and Giza 3 plants under salt and drought stresses, and ABA treatment. The results showed that selected transcripts were differentially expressed under various treatments in both genotypes suggesting their important roles in abiotic stress tolerance responses. The osmotic-responsive genes identified in this study may be considered as potential candidates with a further application as stress selection markers for the creation of faba bean stress-tolerant varieties in various breeding programs.
Disciplines :
Agriculture & agronomy
Author, co-author :
Abid, Ghassen ; Laboratory of Legumes, Biotechnology Center of Borj Cedria, University of Tunis El Manar, Hammam-Lif, Tunisia
Saidi, Mohammed ; Université de Liège - ULiège > Forêts, Nature et Paysage > Gestion des ressources forestières et des milieux naturels ; Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax, Sfax, Tunisia
Ouertani, Rim Nefissi; Laboratory of Plant Molecular Physiology, Biotechnology Center of Borj Cedria, University of Tunis El Manar, Hammam-Lif, Tunisia
Muhovski, Yordan; Department of Life Sciences, Walloon Agricultural Research Centre, Gembloux, Belgium
Jebara, Salwa Harzalli; Laboratory of Legumes, Biotechnology Center of Borj Cedria, University of Tunis El Manar, Hammam-Lif, Tunisia
Ghouili, Emna; Laboratory of Legumes, Biotechnology Center of Borj Cedria, University of Tunis El Manar, Hammam-Lif, Tunisia
Sassi, Khaled; Laboratory of Agronomy, National Agronomy Institute of Tunisia (INAT), University of Carthage, Tunis-Mahrajène, Tunisia
Baudoin, Jean-Pierre ; Université de Liège - ULiège > Département GxABT > Plant Sciences
Ayed, Mohamed El; Laboratory of Bioactive Substances, Biotechnology Center of Borj Cedria, University of Tunis El Manar, Hammam-Lif, Tunisia
Elkahoui, Salem; Laboratory of Bioactive Substances, Biotechnology Center of Borj Cedria, University of Tunis El Manar, Hammam-Lif, Tunisia ; Department of Biology, College of Science, University of Ha’il, Hail, Saudi Arabia
Barhoumi, Fathi; Laboratory of Legumes, Biotechnology Center of Borj Cedria, University of Tunis El Manar, Hammam-Lif, Tunisia
Jebara, Moez; Laboratory of Legumes, Biotechnology Center of Borj Cedria, University of Tunis El Manar, Hammam-Lif, Tunisia
Language :
English
Title :
Differential gene expression reveals candidate genes for osmotic stress response in faba bean (Vicia faba L.) involved in different molecular pathways
Publication date :
March 2021
Journal title :
Acta Physiologiae Plantarum
ISSN :
0137-5881
eISSN :
1861-1664
Publisher :
Springer Science and Business Media Deutschland GmbH
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