[en] The quality of fresh or thawed sperm in stallions has been generally determined by the viability and total and progressive motility of the sperm. Today, the expression of ProAKAP4, a protein present in the flagellum of spermatozoa, appears to be an innovative and relevant functional marker to assess semen quality and male fertility. This study aims to compare the concentration of ProAKAP4 in the semen from 5 stallions frozen with two different extenders immediately after thawing (T0) and 4 h post-thawing (T4). Viability, total and progressive motility were measured in parallel. Significant differences for sperm viability and total motility were observed between the two extenders, as was the concentration of ProAKAP4 both at T0 and T4. At T4, all quality parameters and ProAKAP4 content significantly decreased compared to T0, but with a considerably slower decrease in one extender than the other. These preliminary results suggest that measuring the concentration of ProAKAP4 is a promising tool for the comparison of different extenders and the selection of the optimal freezing medium for each stallion ejaculate.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Blommaert, D; Centre Européen du Cheval, Département Reproduction, Mont-le-Soie, 1, Vielsalm, 6698, Belgium
Sergeant, N; SPQI S.A.S, 82 rue Jeanne d'Arc, 59000, Lille, France, Univ. Lille, Inserm UMRS 1172, Place de Verdun, 59045, Lille, France
Delehedde, M; SPQI S.A.S, 82 rue Jeanne d'Arc, 59000, Lille, France
Donnay, I; Louvain Institute of Biomolecular Science and Technology, Catholic University of Louvain, Louvain-la-Neuve, Belgium
Lejeune, Jean-Philippe ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée ; Centre Européen du Cheval, Département Reproduction, Mont-le-Soie, 1, Vielsalm, 6698, Belgium
Franck, Thierry ; Université de Liège - ULiège > Centres généraux > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Serteyn, Didier ; Université de Liège - ULiège > Centres généraux > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Language :
English
Title :
First results about ProAKAP4 concentration in stallion semen after cryopreservation in two different freezing media.
We thank the Wallonia Region for their financial support (Framework agreement: “ soutien à la filière équine ” (SPW-DGO3- D317046 )). We also thank S. Henkinet, N. Notele for their excellent technical assistance.
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