[en] The hop plant (Humulus lupulus L.) has been exploited for a long time for both its brewing and medicinal uses, due in particular to its specific chemical composition. These last years, hop cultivation that was in decline has been experiencing a renewal for several reasons, such as a craze for strongly hopped aromatic beers. In this context, the present work aims at investigating the genetic and chemical diversity of fifty wild hops collected from different locations in Northern France. These wild hops were compared to ten commercial varieties and three heirloom varieties cultivated in the same sampled geographical area. Genetic analysis relying on genome fingerprinting using 11 microsatellite markers showed a high level of diversity. A total of 56 alleles were determined with an average of 10.9 alleles per locus and assessed a significant population structure (mean pairwise FST = 0.29). Phytochemical characterization of hops was based on volatile compound analysis by HS- SPME GC-MS, quantification of the main prenylated phenolic compounds by UHPLC-UV as well as untargeted metabolomics by UHPLC-HRMS and revealed a high level of chemical diversity among the assessed wild accessions. In particular, analysis of volatile compounds revealed the presence of some minor but original compounds, such as aromadendrene, allo-aromadendrene, isoledene, β-guaiene, α-ylangene and β-pinene in some
wild accessions; while analysis of phenolic compounds showed high content of β-acids in these wild accessions, up to 2.37% of colupulone. Genetic diversity of wild hops previously observed was hence supported by their chemical diversity. Sample soil analysis was also performed to get a pedological classification of these different collection sites. Results of the multivariate statistical analysis suggest that wild hops constitute a huge pool of chemical and genetic diversity of this species.
Disciplines :
Chemistry Agriculture & agronomy
Author, co-author :
Paguet, Anne-Sophie
Siah, Ali
Lefèvre, Gabriel
Moureu, Sophie
Cadalen, Thierry
Samaillie, Jennifer
Michels, Franck ; Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
Deracinois, Barbara
Flahaut, Christophe
Alves Dos Santos, Harmony
Etienne-Debaecker, Audrey
Rambaud, Caroline
Chollet, Sylvie
Molinié, Roland
Fontaine, Jean-Xavier
Waterlot, Christophe
Fauconnier, Marie-Laure ; Université de Liège - ULiège > TERRA Research Centre > Chimie des agro-biosystèmes
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