Safety, Risk, Reliability and Quality; Food Science
Abstract :
[en] Opuntia dillenii (Ker Gawl.) Haw. belongs to the Cactaceae family and is native to the arid and semi-arid regions of Mexico and the southern United States. O. dillenii are now used as medicinal plants in various countries. In this study, we investigated the chemical composition of ethanolic extracts obtained from seeds, juice, and peel of O. dillenii fruits collected from Morocco, and we evaluated their antibacterial, antifungal, and antidiabetic activities. Phytochemical screening revealed high quantities of polyphenols (193.73 ± 81.44 to 341.12 ± 78.90 gallic acid eq [g/100 g dry weight]) in the extracts. The major phenolic compounds determined by HPLC were gallic acid, vanillic acid, and syringic acid. Regarding flavonoids, quercetin 3-O-β-D-glucoside and kaempferol were the predominant molecules. Juice extracts showed weak to moderate antibacterial activity against the bacteria species Listeria monocytogenes, Escherichia coli, and Salmonella braenderup. All tested extracts displayed a significant inhibitory effect on α-glucosidase and α-amylase activities in vitro, with the peel extracts showing the greatest inhibitory effects. Together, these findings suggest that O. dillenii fruits are a promising source for the isolation of novel compounds with antibacterial or antidiabetic activities. For the most abundant phytochemicals identified in O. dillenii peel ethanolic extract, molecular docking simulations against human pancreatic α-amylase enzyme were performed. These indicated the presence of bioactive compounds in the extract with a better potential to decrease the enzyme activity than the commercial drug acarbose.
Loukili, EL Hassania ; Laboratory of Applied and Environmental Chemistry (LCAE), Mohammed First University, Faculty of Sciences, B.P. 717 60000, Oujda, Morocco
Bouchal, Btissam ; Genetics Unit, Medical Sciences Research Laboratory, Faculty of Medicine and Pharmacy of Oujda, University Mohammed Premier, Oujda, Morocco
Bouhrim, Mohamed ; Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Mohammed First University, Faculty of Sciences, B.P. 717 60000, Oujda, Morocco
Abrigach, Farid ; Laboratory of Applied and Environmental Chemistry (LCAE), Mohammed First University, Faculty of Sciences, B.P. 717 60000, Oujda, Morocco
Genva, Manon ; Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
Zidi, Kahina ; Laboratory of Applied Biochemistry, Faculty of Natural Sciences and Life, University of Bejaia, Targa-Ouezmour, 06000 Bejaia, Algeria
Bnouham, Mohamed ; Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Mohammed First University, Faculty of Sciences, B.P. 717 60000, Oujda, Morocco
Bellaoui, Mohammed ; Genetics Unit, Medical Sciences Research Laboratory, Faculty of Medicine and Pharmacy of Oujda, University Mohammed Premier, Oujda, Morocco
Hammouti, Belkheir ; Laboratory of Applied and Environmental Chemistry (LCAE), Mohammed First University, Faculty of Sciences, B.P. 717 60000, Oujda, Morocco
Addi, Mohamed ; Laboratory of Agricultural Production Improvement, Biotechnology and Environment, Faculty of Sciences, Mohammed I University, BP-717, 60000 Oujda, Morocco
Ramdani, Mohammed ; Laboratory of Applied and Environmental Chemistry (LCAE), Mohammed First University, Faculty of Sciences, B.P. 717 60000, Oujda, Morocco
Fauconnier, Marie-Laure ; Université de Liège - ULiège > TERRA Research Centre > Chimie des agro-biosystèmes
Language :
English
Title :
Chemical Composition, Antibacterial, Antifungal and Antidiabetic Activities of Ethanolic Extracts of Opuntia dillenii Fruits Collected from Morocco
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