[en] Vegetables and fruits can potentially accumulate cyanotoxins after water contaminated with cyanobacteria is used for irrigation. We developed and validated an analytical method to quantify eight microcystin congeners (MCs) and nodularin (NOD) using ultra high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) in three different matrices. Strawberries, carrots and lettuce are selected as model matrices to represent the fruits/berries, leafy and root vegetables, sequentially. The validation of a UHPLC-MS/MS method in the strawberry matrix is novel. Matrix effects are observed in all three matrices. Our methodology uses matrix-matched calibration curves to compensate for the matrix effect. The implementation of our method on 103 samples, containing nine different sorts of fruits and vegetables from the Belgian market, showed no presence of MCs or NOD. However, the recoveries of our quality controls showed the effectiveness of our method, illustrating that the use of this method in future research or monitoring as well as in official food controls in fruit and vegetable matrices is valid.
Disciplines :
Food science Chemistry Public health, health care sciences & services
Author, co-author :
Van Hassel, Wannes ; Université de Liège - ULiège > Integrative Biological Sciences (InBioS)
Masquelier, Julien
Andjelkovic, Mirjana
Rajkovic, Andreja
Language :
English
Title :
Towards a Better Quantification of Cyanotoxins in Fruits and Vegetables: Validation and Application of an UHPLC-MS/MS-Based Method on Belgian Products
Publication date :
20 October 2022
Journal title :
Separations
eISSN :
2297-8739
Publisher :
MDPI AG
Special issue title :
Development of Highly Efficient Separation-Based Analytical Methods for Food Integrity Assurance
H2020 - 965173 - Imptox - AN INNOVATIVE ANALYTICAL PLATFORM TO INVESTIGATE THE EFFECT AND TOXICITY OF MICRO AND NANO PLASTICS COMBINED WITH ENVIRONMENTAL CONTAMINANTS ON THE RISK OF ALLERGIC DISEASE IN PRECLINICAL AND CLINICAL
Name of the research project :
SP 21/5 CYANTIR 1
Funders :
FPS Health Federal Public Service Health, Food Chain Safety and Environment [BE] EU - European Union [BE]
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