Bacterial community composition in Lake Tanganyika: Vertical and horizontal heterogeneity

[en] Vertical and latitudinal differences in bacterial community composition (BCC) in Lake Tanganyika were studied during the dry season of 2002 by means of denaturing gradient gel electrophoresis analysis of PCR-amplified 16S RNA fragments. Dominant bands were sequenced and identified as members of the Cyanobacteria, Actinobacteria, Nitrospirae, green nonsulfur bacteria, and Firmicutes divisions and the Gamma- and Deltaproteobacteria subdivisions. The BCC in the lake displayed both vertical and latitudinal variation. Vertical changes in BCC were related to the thermal water column stratification, which influences oxygen and nutrient concentrations. Latitudinal variation was related to upwelling of deep water and increased primary production in the south of the lake. The number of bands per sample increased with bacterial production in the epilimnion of the lake, suggesting a positive diversity-productivity relationship. Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Disciplines :

Aquatic sciences & oceanology

Author, co-author :

De Wever, A.; Ghent University, Department of Biology, Section of Protistology and Aquatic Ecology, 9000 Ghent, Belgium

Muylaert, K.; Ghent University, Department of Biology, Section of Protistology and Aquatic Ecology, 9000 Ghent, Belgium

Van Der Gucht, K.; Ghent University, Department of Biology, Section of Protistology and Aquatic Ecology, 9000 Ghent, Belgium

Pirlot, S.; University of Namur, Department of Biology, URBO, Namur, Belgium

Cocquyt, C.; Ghent University, Department of Biology, Section of Protistology and Aquatic Ecology, 9000 Ghent, Belgium

Descy, Jean-Pierre ; Université de Liège - ULiège > Département d'astrophysique, géophysique et océanographie (AGO) > Chemical Oceanography Unit (COU) ; University of Namur, Department of Biology, URBO, Namur, Belgium

Plisnier, Pierre-Denis ; Université de Liège - ULiège > Département d'astrophysique, géophysique et océanographie (AGO) > Chemical Oceanography Unit (COU) ; University of Namur, Department of Biology, URBO, Namur, Belgium, Royal Museum for Central Africa, Tervuren, Belgium

Vyverman, W.; Ghent University, Department of Biology, Section of Protistology and Aquatic Ecology, 9000 Ghent, Belgium

Language :

English

Title :

Bacterial community composition in Lake Tanganyika: Vertical and horizontal heterogeneity

Publication date :

2005

Journal title :

Applied and Environmental Microbiology

ISSN :

0099-2240

eISSN :

1098-5336

Publisher :

American Society for Microbiology, Washington, Us dc

Volume :

Issue :

Pages :

5029 - 5037

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 18 October 2022

Statistics

Number of views

104 (1 by ULiège)

Number of downloads

24 (0 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

100

Bibliography

Ahn, T. S., S. H. Hong, D. J. Kim, J. H. Suck, and V. V. Drucker. 1999. The bacterial community of southern Lake Baikal in winter. J. Microbiol. 37:10-13.
Altschul, S., T. Madden, A. Schaffer, J. H. Zhang, Z. Zhang, W. Miller, and D. Lipman. 1998. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Faseb. J. 12:A1326-A1326.
Bel'kova, N. L., V. V. Parfenova, T. Y. Kostornova, L. Y. Denisova, and E. F. Zaichikov. 2003. Microbial biodiversity in the water of Lake Baikal. Microbiology 72:203-212.
Coulter, G. W. (ed.). 1991. Lake Tanganyika and its life. Oxford University Press, New York, N.Y.
Crump, B. C., G. W. Kling, M. Bahr, and J. E. Hobbie. 2003. Bacterioplankton community shifts in an arctic lake correlate with seasonal changes in organic matter source. Appl. Environ. Microbiol. 69:2253-2268.
Denisova, L. Y., N. L. Bel'kova, I. I. Tulokhonov, and E. F. Zaichikov. 1999. Bacterial diversity at various depths in the southern part of Lake Baikal as revealed by 16S rDNA sequencing. Microbiology 68:475-483.
Descy, J.-P., M.-A. Hardy, S. Sténuite, S. Pirlot, B. Leporcq, I. Kimirei, B. Sekadende, S. R. Mwaitega, and D. Sinyenza. 2005. Phytoplankton pigments and community composition in Lake Tanganyika. Freshwater Biol. 50:668-684.
Descy, J. P., H. W. Higgins, D. J. Mackey, J. P. Hurley, and T. M. Frost. 2000. Pigment ratios and phytoplankton assessment in northern Wisconsin lakes. J. Phycol. 36:274-286.
Eder, W., and R. Huber. 2002. New isolates and physiological properties of the Aquificales and description of Thermocrinis albus sp. nov. Extremophiles 6:309-318.
Elsgaard, L., D. Prieur, G. M. Mukwaya, and B. B. Jorgensen. 1994. Thermophilic sulfate reduction in hydrothermal sediment of Lake Tanganyika, East Africa. Appl. Environ. Microbiol. 60:1473-1480.
Fuhrman, J. A., and F. Azam. 1980. Bacterioplankton secondary production estimates for coastal waters of British Columbia, Antarctica, and California. Appl. Environ. Microbiol. 39:1085-1095.
Fuhrman, J. A., and F. Azam. 1982. Thymidine incorporation as a measure of heterotrophic bacterioplankton production in marine surface waters: evaluation and field results. Mar. Biol. 66:109-120.
Gasol, J. M., M. Comerma, J. C. Garcia, J. Armengol, E. O. Casamayor, P. Kojecka, and K. Ŝimek. 2002. A transplant experiment to identify the factors controlling bacterial abundance, activity, production, and community composition in a eutrophic canyon-shaped reservoir. Limnol. Oceanogr. 47:62-77.
Greenberg, A. E., American Public Health Association, American Water Works Association, and Water Environment Federation (ed.). 1992. Standard methods for the examination of water and wastewater, 18th ed. American Public Health Association, Washington, D.C.
Hecky, R. E., and H. J. Kling. 1981. The phytoplankton and proto-zooplankton of the euphotic zone of Lake Tanganyika-species composition, biomass, chlorophyll content, and spatio-temporal distribution. Limnol. Oceanogr. 26:548-564.
15a. Horner-Devine, M. C, M. A. Leibold, V. H. Smith, and B. J. M. Bohannon. 2003. Bacterial diversity patterns along a sradient of primary productivity. Ecol. Lett. 6:613-622.
Inagaki, F., M. Suzuki, K. Takai, H. Oida, T. Sakamoto, K. Aoki, K. H. Nealson, and K. Horikoshi. 2003. Microbial communities associated with geological horizons in coastal subseafloor sediments from the Sea of Okhotsk. Appl. Environ. Microbiol. 69:7224-7235.
Reference deleted.
Kerkhof, L. J., M. A. Voytek, R. M. Sherrell, D. Millie, and O. Schofield. 1999. Variability in bacterial community structure during upwelling in the coastal ocean. Hydrobiologia 401:139-148.
Kirchman, D. L., and D. Castle. 2004. Composition of estuarine bacterial communities assayed by denaturing gradient gel electrophoresis and fluorescence in situ hybridization. Limnol. Oceanogr. Methods 2:303-314.
Koizumi, Y., H. Kojima, and M. Fukui. 2004. Dominant microbial composition and its vertical distribution in saline meromictic Lake Kaiike (Japan) as revealed by quantitative oligonucleotide probe membrane hybridization. Appl. Environ. Microbiol. 70:4930-4940.
Konopka, A., T. Bercot, and C. Nakatsu. 1999. Bacterioplankton community diversity in a series of thermally stratified lakes. Microb. Ecol. 38:126-135.
Kurata, S., T. Kanagawa, Y. Magariyama, K. Takatsu, K. Yamada, T. Yokomaku, and Y. Kamagata. 2004. Reevaluation and reduction of a PCR bias caused by reannealing of templates. Appl. Environ. Microbiol. 70:7545-7549.
Lindström, E. S., K. Vrede, and E. Leskinen. 2004. Response of a member of the Verrucomicrobia, among the dominating bacteria in a hypolimnion, to increased phosphorus availability. J. Plankton Res. 26:241-246.
Reference deleted.
Lysnes, K., I. H. Thorseth, B. O. Steinsbu, L. Ovreas, T. Torsvik, and R. B. Pedersen. 2004. Microbial community diversity in seafloor basalt from the arctic spreading ridges. FEMS Microbiol. Ecol. 50:213-230.
MacIsaac, E. A., and J. G. Stockner. 1993. Enumeration of phototrophic picoplankton by autofluorescence microscopy, p. 187-197. In P. F. Kemp, B. F. Sherr, E. B. Sherr, and J. J. Cole (ed.), Handbook of methods in aquatic microbial ecology. Lewis Publishers, Boca Raton, Fla.
Malinsky-Rushansky, N. Z., and C. Legrand. 1996. Excretion of dissolved organic carbon by phytoplankton of different sizes and subsequent bacterial uptake. Mar. Ecol. Prog. Ser. 132:249-255.
Menden-Deuer, S., and E. J. Lessard. 2000. Carbon to volume relationships for dinoflagellates, diatoms, and other protist plankton. Limnol. Oceanogr. 45:569-579.
Montagnes, D. J. S., and D. H. Lynn. 1993. A quantitative protargol stain (QPS) for ciliates and other protists, p. 229-240. In P. F. Kemp, B. F. Sherr, E. B. Sherr, and J. J. Cole (ed.). Handbook of methods in aquatic microbial ecology. Lewis Publishers, Boca Raton. Fla.
Muyzer, G., E. C. Dewaal, and A. G. Uitterlinden. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S ribosomal RNA. Appl. Environ. Microb. 59:695-700.
Nold, S. C., and G. Zwart. 1998. Patterns and governing forces in aquatic microbial communities. Aquat. Ecol. 32:17-35.
Pandolfini, E., I. Thys, B. Leporcq, and J. P. Descj. 2000. Grazing experiments with two freshwater zooplankters: fate of chlorophyll and carotenoid pigments. J. Plankton Res. 22:305-319.
Pinhassi, J., A. Winding, S. J. Binnerup, U. L. Zweifel, B. Riemann, and A. Hagstrom. 2003. Spatial variability in bacterioplankton community composition at the Skagerrak-Kattegat front. Mar. Ecol. Prog. Ser. 255:1-13.
Plisnier, P.-D. 1998. Lake Tanganyika: recent climate changes and teleconnections with ENSO, p. 228-250. In G. Demarée, J. Alexandre, and M. De Dapper (ed.), Proceedings of the International Conference "Tropical Climatology, Meteorology and Hydrology." Royal Academy of Overseas Sciences and Royal Meteorological Institute of Belgium, Brussels, Belgium.
Plisnier, P.-D., D. Chitamwebwa, L. Mwape, K. Tshibangu, V. Langenberg, and E. Coenen. 1999. Limnological annual cycle inferred from physical-chemical fluctuations at three stations of Lake Tanganyika. Hydrobiologia 407:45-58.
Plisnier, P.-D., and E. J. Coenen. 2001. Pulsed and dampened annual limnological fluctuations in Lake Tanganyika, p. 83-96. In M. Munawar and R. E. Hecky (ed.), The great lakes of the world (GLOW): food-web, health and integrity. Backhuys Publishers, Leiden, The Netherlands.
Porter, K. G., and Y. S. Feig. 1980. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr. 25:943-948.
Putt, M., and D. K. Stoecker. 1989. An experimentally determined carbon-volume ratio for marine oligotrichous ciliates from estuarine and coastal waters. Limnol. Oceanogr. 34:1097-1103.
Sarvala, J., K. Salonen, M. Jarvinen, E. Aro, T. Huttula, P. Kotilainen, H. Kurki, V. Langenberg, P. Mannini, A. Peltonen, P.-D. Plisnier, I. Vuorinen, H. Molsa, and O. V. Lindqvist. 1999. Trophic structure of Lake Tanganyika; carbon flows in the pelagic food web. Hydrobiologia 407:149-173.
39a. Schauer, M., R. Massana, and C. Pedros-Alio. 2000. Spatial differences in bacterioplankton composition along the Catalan coast (NW Mediterranean) assessed by molecular fingerprinting. FEMS Microbiol. Ecol. 33:51-59.
Semenova, E. A., K. D. Kuznedelov, and M. A. Grachev. 2001. Nucleotide sequences of fragments of 16S rRNA of the Baikal natural populations and laboratory cultures of cyanobacteria. Mol. Biol. 35:405-410.
Sherr, E. B., and B. F. Sherr. 1993. Preservation and storage of samples for enumeration of heterotrophic protists, p. 207-212. In P. F. Kemp, B. F. Sherr, E. B. Sherr, and J. J. Cole (ed.), Handbook of methods in aquatic microbial ecology. Lewis Publishers, Boca Raton, Fla.
Sievert, S. M., T. Brinkhoff, G. Muyzer, V. Ziebis, and J. Kuever. 1999. Spatial heterogeneity of bacterial populations along an environmental gradient at a shallow submarine hydrothermal vent near Milos Island (Greece). Appl. Environ. Microbiol. 65:3834-3842.
Simpson, J. H., P. B. Tett, M. L. Argoteespinoza, A. Edwards, K. J. Jones, and G. Savidge. 1982. Mixing and phytoplankton growth around an island in a stratified sea. Cont. Shelf Res. 1:15-31.
Swofford, D. L. 1999. PAUP*: phylogenetic analysis using parsimony (* and other methods), version 4. Sinauer Associates, Sunderland, Mass.
Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin, and D. G. Higgins. 1997. The ClustalX Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25:
Urbach, E., K. L. Vergin, L. Young, A. Morse, G. L. Larson, and S. J. Giovannoni. 2001. Unusual bacterioplankton community structure in ultraoligotrophic Crater Lake. Limnol. Oceanogr. 46:557-572.
Van der Gucht, K., K. Sabbe, L. De Meester, N. Vloemans, G. Zwart, M. Gillis, and W. Vyverman. 2001. Contrasting bacterioplankton community composition and seasonal dynamics in two neighbouring hypertrophic freshwater lakes. Environ. Microbiol. 3:680-690.
van Hannen, E. J., W. M. Mooij, M. P. van Agterveld, H. J. Gons, and H. J. Laanbroek. 1999. Detritus-dependent development of the microbial community in an experimental system: qualitative analysis by denaturing gradient gel electrophoresis. Appl. Environ. Microbiol. 65:2478-2484.
Warnecke, F., R. Amann, and J. Pernthaler. 2004. Actinobacterial 16S rRNA genes from freshwater habitats cluster in four distinct lineages. Environ. Microbiol. 6:242-253.
Yannarell, A. C, and E. W. Triplett. 2004. Within- and between-lake variability in the composition of bacterioplankton communities: investigations using multiple spatial scales. Appl. Environ. Microbiol. 70:214-223.
Zwart, G., B. C. Crump, M. Agterveld, F. Hagen, and S. K. Han. 2002. Typical freshwater bacteria: an analysis of available 16S rRNA gene sequences from plankton of lakes and rivers. Aquat. Microb. Ecol. 28:141-155.