[en] Saffron is a spice derived from the flower of Crocus sativus L., which has a special aroma, colour and odour influencing positively its economic value. In this context, ten saffron ecotypes were screened for their biochemical composition and antioxidant activity. The samples were also analysed using GC-MS and LC-MS to determine their content of volatile and phenolic compounds, respectively. The results revealed statistically significant differences among samples based on moisture (9.09%-11.23%), total phenols (31.62-62.71 mg EAG/g), total flavonoids (23.02-40.02 mg ER/mg), total carotenoids (66.12-155.05 μg/g), picrocrocin (88.99-121.53), crocin (137.44-228.39) and safranal (26.56-53.04). The radical scavenging activity ranged from 17.09% to 29.53% for DPPH assay, and oscillated from 0.128 mmol AAE/g to 0.239 mmol AAE/g for ABTS test, while the ferric reducing antioxidant potency (FRAP) varied from 0.974 to 1.989 mmol Fe2+/g. Gas chromatography-mass spectrometry (GC-MS) analysis identified 66 volatile compounds, among which the Safranal and Isophorone were the most abondant. The ES1 from Taliouine recorded a very distinct volatile composition compared to the others ecotypes with 22 authentic volatile compounds. Moreover, liquid chromatography- mass spectrometry (LC-MS) analysis revealed 14 phenolic compounds with picrocrocin and crocin were found to be the major compounds. The principal component analysis classified the investigated ecotypes into two mean distinctive sets with ES1 and ES9 were distinguished as a single items. The α-pinene, β-pinene, limonene, anethole, acetic acid, ketoisophorone, isophorone, safranal, thymoquinone, total flavonoids, FRAP and total carotenoids, are the main discriminant variables. The two-dimensional analysis of the clustered heatmaps divided showed a relatively similar patterns as the principal component analysis (PCA) and confirmed the singularity of the sample ES1 based on its particular volatile profile dominated mainly by α-terpinyl acetate, methyleugenol, copaene, anethole, limonene, methyl-cyclopentane, which were not identified in the other samples even at minor levels. These findings herein found revealed the high quality of Moroccan saffron, which is very important for the species breeding and valorization.
Disciplines :
Chemistry Agriculture & agronomy
Author, co-author :
Kabiri, Ghizlane; Laboratory of Industrial Engineering and Surface Engineering, Faculty of Science and Techniques Sultan Moulay Slimane University Beni Mellal Morocco
Hssaini, Lahcen; National Institute of Agricultural Research Rabat Morocco
Naim, Nadia; National School of Agriculture Meknes Morocco
Houmanat, Karim; National Institute of Agricultural Research Rabat Morocco
Ennahli, Said; National School of Agriculture Meknes Morocco
Fauconnier, Marie-Laure ; Université de Liège - ULiège > TERRA Research Centre > Chimie des agro-biosystèmes
Hanine, Hafida; Laboratory of Industrial Engineering and Surface Engineering, Faculty of Science and Techniques Sultan Moulay Slimane University Beni Mellal Morocco
Language :
English
Title :
Aromatic potential, quality and antioxidant activity of saffron grown in Morocco
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Bibliography
Serrano-Díaz J, Sánchez AM, Martínez-Tomé M, Winterhalter P, Alonso GL. A contribution to nutritional studies on Crocus sativus flowers and their value as food. J Food Compos Anal. 2013;31:101-108.
Alavizadeh SH, Hosseinzadeh H. Bioactivity assessment and toxicity of crocin: A comprehensive review. Food Chem Toxicol. 2014;64:65-80. doi:10.1016/j.fct.2013.11.016
Vavilov NI. The origin, variation, immunity and breeding of cul- tivated plants. The Cronica Botanica, Co.; 1951. doi:10.1126/science.115.2990.433.b
Tammaro F. Crocus sativus L. Cv. Piano di Navelli (L'Aquila saffron): Environment, cultivation, morphometric characteristics, active principles, uses, in Proceedings of the International Conference on Saffron (Crocus sativus L.), L'Àquilla, Italy. October 27-29, 1989; pp 47-57.
Lage M, Faiz C, Cantrell CL. Developmental project for introducing saffron (Crocus sativus L.) as an alternative crop in other Moroccan regions, in Proceedings of the 2nd International Symposium on Saffron Biology and Technology, Masshad, Iran, October 28-30. 2006; pp. 49-52.
Ganaie DB, Singh Y. Cultivation practices and waning production of saffron in Jammu & Kashmir. Agri Res & Tech. 2019;20:148-153.
Abdullaev FI, Espinosa-Aguirre JJ. Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials. Cancer Detect Prev. 2004;28:426-432. doi:10.1016/j.cdp.2004.09.002
Al-Mofleh LA, Alhaider AA, Mossa JS, Al-Sohaibani MO, Qureshi S, Rafatullah S. Antigastric ulcer studies on 'saffron' Crocus sativus L. in rats. Paki J Biol Sci. 2006;9(6):1009-1013. doi:10.3923/pjbs.2006.1009.1013
Mzabri I, Chetouani M, Charif K, Addi M, Nkengurutse J. The Agro-morphological behavior of saffron under the environmental conditions of four areas in Eastern Morocco. Mater Today Proc. 2019;13:1062-1069. doi:10.1016/j.matpr.2019.04.072
Aboudrare A, Aw-Hassan A, Lybbert TJ. Importance Socio-économique du Safran pour les Ménages des Zones de Montagne de la Région de Taliouine-Taznakht au Maroc. Rev Mar Sci Agron Vét. 2014;2:5-14.
Mzabri I, Charif K, Rimani M, Kouddane N, Boukroute A, Berrichi A. History, biology, and culture of Crocus sativus: Overview and perspectives. Arab J Chem Enviro Res. 2021;08:01-28.
ISO/TS 3632-2. “Safran (Crocus sativus L.)- Partie 1: spécifications, Partie 2: Méthodes d'essai: International Organisation for Standardization, 2003.
Tajik S, Zarinkamar F, Soltani BM, Nazari M. Induction of phenolic and flavonoid compounds in leaves of saffron (Crocus sativus L.) by salicylic acid. Sci Hortic. 2019;257:108751. doi:10.1016/j.scienta.2019.108751
Lamaison JL, Carnat A. Teneurs en principaux flavonoids des fleurs de Crataegeus monogyna Jacq et de Crataegeus laevigata (Poiret, D. C) en fonction de la végétation. Pharm Acta Helv. 1999;65:315-320.
Lahmass I, Ouahhoud S, Elmansuri M, et al. Determination of antioxidant properties of six by-products of Crocus sativus L. (Saffron) Plant Products. Waste Biomassn Valorisation. 2018;9:1349-1357. doi:10.1007/s12649-017-9851-y
Tuberoso CIG, Boban M, Bifulco E, Budimir D, Pirisi FM. Antioxidant capacity and vasodilatory properties of Mediterranean food: the case of Cannonau wine, myrtle berries liqueur and strawberry-tree honey. Food Chem. 2013;140(4):686-691. doi:10.1016/j.foodchem.2012.09.071
Urbani E, Blasi F, Simonetti MS, Chiesi C, Cossignani L. Investigation on secondary metabolite content and antioxidant activity of commercial saffron powder. Eur Food Res Technolo. 2016;242(6):987-993. doi:10.1007/s00217-016-2687-z
Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of 'antioxidant power': The FRAP assay. Anal Biochem. 1996;239(1):70-76. doi:10.1006/ABIO.1996.0292
Pulido R, Bravo L, Saura-Calixto F. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. J Agric Food Chem. 2000;48(8):3396-3402. doi:10.1021/jf9913458
Baba SA, Malik AH, Wani ZA, et al. Phytochemical analysis and antioxidant activity of different tissue types of Crocus sativus and oxidative stress alleviating potential of saffron extract in plants, bacteria and yeast. S Afr J Bot. 2015;99:80-87. doi:10.1016/j.sajb.2015.03.194
Belyagoubi L, Loukidi B, Belyagoubi-Benhammo N, et al. Valorization of Algerian Saffron: Stigmas and Flowers as Source of Bioactive Compounds. Waste Biomass Valorization. 2021;12:1-13. doi:10.1007/s12649-021-01454-6
Caser M, Demasi S, Stelluti S, Donno D, Scariot V. Crocus sativus L. Cultivation in alpine environments: Stigmas and tepals as source of Bioactive Compounds. Agronomy. 2020;10:1473. doi:10.3390/agronomy10101473
Azghandi Fardaghi A, Es-haghia A, Feizy J, Lakshmipathy R. Antioxidant capacity and chemical composition of different parts of saffron flowers. J Food Bioprocess Eng. 2021;4(1):69-74. doi:10.22059/JFABE.2021.316326.1078
Hadizadeh F, Mahdavi M, Emami SA, et al. Evaluation of ISO method in saffron qualification. In II International Symposium on Saffron Biology and Technology. 2006;739:405-410.
Nazarian R, Samim N, Jami MQ. Determination of saffron (Crocus sativus L.) quality characteristics in Herat–Afghanistan. J Hortic Postharvest Res. 2021;13:11-18. doi:10.22077/jhpr.2021.3663.1168
Atyane LH, Molinet J, Serghini MA, Dupuy N, Maimouni LEL. Influence of drying process on safranal content in the Taliouine Saffron (Morocco): quantification by gas chromatography J Mater. Environ Sci. 2017;8(S):4597-4603.
Samaha H, Chahine N, Sobolev AP, Menghini L, Makhlouf H. 1H-NMR Metabolic Profiling and Antioxidant Activity of Saffron (Crocus sativus) Cultivated in Lebanon. Molecules. 2021;26:4906. doi:10.3390/molecules26164906
Ordoudi SA, Befani CD, Nenadis N, Koliakos GG, Tsimidou MZ. Further examination of antiradical properties of Crocus sativus stigmas extract rich in crocins. J Agric Food Chem. 2009;57:3080-3086. doi:10.1021/jf804041g
Tuberoso CI, Rosa A, Montoro P, Fenu MA, Pizza C. Antioxidant activity, cytotoxic activity and metabolic profiling of juices obtained from saffron (Crocus sativus L.) floral by-products. Food Chem. 2016;199:18-27. doi:10.1016/j.foodchem.2015.11.115
Sevindik B. Stability of volatile compounds of Turkish saffron (Crocus sativus) after one-year storage. J Raw Mater Processed Food. 2020;1(2):72-79.
Karabagias I, Liakou V, Koutsoubou M, Kontakos S, Kontominas M. Characterization and geographical discrimination of Saffron from Greece, Spain, Iran, and Morocco based on volatile and bioactivity markers, using chemometrics. Eur Food Res Technol. 2017;243:1577-1591. doi:10.1007/s00217-017-2866-6
Azarabadi N, Özdemir F. Determination of crocin content and volatile components in different qualities of iranian saffron. GIDA the journal of food. 2018;43(3):476-489.
Crozet A, De Sus-rousset H, De Durfort SJ. Crocus sativus L.(Iridaceae), Saffron (I). Phytothérapie. 2012;10:121-125. doi:10.15237/gida.GD18018
Tsimidou M, Biliaderis CG. Kinetic studies of Saffron (Crocus sativus L.) quality deterioration. J Agric Food Chem. 1997;45(8):2890-2898. doi:10.1021/jf970076n
Aiello D, Siciliano C, Mazzotti F, Di Donna L, Athanassopoulos CM, Napoli A. Molecular species fingerprinting and quantitative analysis of saffron (Crocus sativus L.) for quality control by MALDI mass spectrometry. Royal Society of. Chemistry. 2018;8:36104-36113. doi:10.1039/C8RA07484D
Kalinowska M, Gryko K, Wróblewska AM, Jabłonska-Trypuc A, Karpowicz D. Phenolic content, chemical composition and anti−/pro-oxidant activity of Gold Milenium and Papierowka apple peel extracts. Sci Rep. 2020;10:14915. doi:10.1038/s41598-020-71351-w
Terpinc P, Čeh B, Ulrih NP, Abramovič H. Studies of the correlation between antioxidant properties and the total phenolic content of different oil cake extracts. Ind Crops Prod. 2012;39:210-217. doi:10.1016/j.indcrop.2012.02.023
Hssaini L, Hernandez F, Viuda-Martos M, et al. Survey of phenolic acids, flavonoids and in vitro antioxidant potency between fig peels and pulps: Chemical and chemometric approach. Molecules. 2021;26:2574. doi:10.3390/molecules26092574
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