[en] Intramuscular fat (IMF) content is one of the most important factors determining beef quality and price. Intramuscular adipocytes develop from mesenchymal stem cells (MSCs) in mesoderm. The mechanisms of preadipocytes differentiate into mature adipocytes to a great extent are clear, but the commitment of MSCs to preadipocytes is largely unknown. In this study, the Platelet-derived growth factor receptor α (PDGFRα) positive progenitor cells were isolated from the longissimus dorsi muscle (LM) of fetal bovine and induced adipogenesis. To optimize the in vitro IMF differentiation model, the effects of bone morphogenic protein 4 (BMP4) and rosiglitazone during differentiation were studied. Comparing with control group, progenitor cells treated with BMP4 or rosiglitazone accumulated more intracellular lipid. Furthermore, the mRNA expression level of adipocyte-specific genes also increased significantly in BMP4 or rosiglitazone treated cells. The result indicated that BMP4 and rosiglitazone could promote adipogenesis and be applied in adipogenic differentiation of fetal bovine derived progenitor cells.
Disciplines :
Animal production & animal husbandry Genetics & genetic processes
Author, co-author :
Wang, Yahui; Key Laboratory of Animal Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
Ren, Ling; Key Laboratory of Animal Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
Xing, Yishen; Key Laboratory of Animal Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
Hu, Xin ; Université de Liège - ULiège > TERRA Research Centre ; Key Laboratory of Animal Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
Li, Qian; Key Laboratory of Animal Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
Xu, Lingyang; Key Laboratory of Animal Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
Li, Junya; Key Laboratory of Animal Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
Zhang, Lupei; Key Laboratory of Animal Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
Language :
English
Title :
BMP4 and rosiglitazone improves adipogenesis of bovine fetal muscle derived progenitor cells
Publication date :
February 2021
Journal title :
Pakistan Journal of Zoology
ISSN :
0030-9923
Publisher :
University of Punjab (new Campus)
Volume :
53
Issue :
1
Pages :
395 - 398
Peer reviewed :
Peer reviewed
Funding text :
Animal experiments were conducted according to the guidelines established by the Regulations for the Administration of Affairs Concerning Experimental Animals (Ministry of Science and Technology, China). All animal protocols were approved by Animal Ethics Committee of Institute of Animal Sciences, Chinese Acadamy of Agricultrual Sciences. Pregnant cows were raised in Inner Mongolia Aokesi Agriculture Co., Ltd (Wulagai, China). All efforts were made to minimize the cow’s suffering. This work was supported by National Natural Science Foundation of China (31672384), the Cattle Breeding Innovative Research Team (ASTIPIAS03 and ZDXT2018006).Animal experiments were conducted according to the guidelines established by the Regulations for the Administration of Affairs Concerning Experimental Animals (Ministry of Science and Technology, China). All animal protocols were approved by Animal Ethics Committee of Institute of Animal Sciences, Chinese Acadamy of Agricultrual Sciences. Pregnant cows were raised in Inner Mongolia Aokesi Agriculture Co., Ltd (Wulagai, China). All efforts were made to minimize the cow's suffering. This work was supported by National Natural Science Foundation of China (31672384), the Cattle Breeding Innovative Research Team (ASTIPIAS03 and ZDXT2018006).
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