[en] BACKGROUND: miRNAs play critical roles in growth and development. Various studies of chicken muscle development have focused on identifying miRNAs that are important for embryo or adult muscle development. However, little is known about the role of miRNAs in the whole muscle development process from embryonic to post-hatching periods. Here, we present a comprehensive investigation of miRNA transcriptomes at 12-day embryo (E12), E17, and day 1 (D1), D14, D56 and D98 post-hatching stages.
RESULTS: We identified 337 differentially expressed miRNAs (DE-miRNAs) during muscle development. A Short Time-Series Expression Miner analysis identified two significantly different expression profiles. Profile 4 with downregulated pattern contained 106 DE-miRNAs, while profile 21 with upregulated pattern contained 44 DE-miRNAs. The DE-miRNAs with the upregulated pattern mainly played regulatory roles in cellular turnover, such as pyrimidine metabolism, DNA replication, and cell cycle, whereas DE-miRNAs with the downregulated pattern directly or indirectly contributed to protein turnover metabolism such as glycolysis/gluconeogenesis, pyruvate metabolism and biosynthesis of amino acids.
CONCLUSIONS: The main functional miRNAs during chicken muscle development differ between embryonic and post-hatching stages. miRNAs with an upregulated pattern were mainly involved in cellular turnover, while miRNAs with a downregulated pattern mainly played a regulatory role in protein turnover metabolism. These findings enrich information about the regulatory mechanisms involved in muscle development at the miRNA expression level, and provide several candidates for future studies concerning miRNA-target function in regulation of chicken muscle development.
Disciplines :
Animal production & animal husbandry Genetics & genetic processes
Author, co-author :
Liu, Jie; Shandong Academy of Agricultural Sciences, Poultry Institute, Ji'nan, 250023, China ; Poultry Breeding Engineering Technology Center of Shandong Province, Ji'nan, 250023, China
Li, Fuwei; Shandong Academy of Agricultural Sciences, Poultry Institute, Ji'nan, 250023, China ; Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Ji'nan, 250023, China
Hu, Xin ; Université de Liège - ULiège > TERRA Research Centre ; Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Beijing, 100193, China
Cao, Dingguo; Shandong Academy of Agricultural Sciences, Poultry Institute, Ji'nan, 250023, China ; Poultry Breeding Engineering Technology Center of Shandong Province, Ji'nan, 250023, China
Liu, Wei; Shandong Academy of Agricultural Sciences, Poultry Institute, Ji'nan, 250023, China
Han, Haixia; Shandong Academy of Agricultural Sciences, Poultry Institute, Ji'nan, 250023, China
Zhou, Yan; Shandong Academy of Agricultural Sciences, Poultry Institute, Ji'nan, 250023, China. sally7919@163.com ; Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Ji'nan, 250023, China. sally7919@163.com
Lei, Qiuxia; Shandong Academy of Agricultural Sciences, Poultry Institute, Ji'nan, 250023, China. lei_qiuxia@163.com ; Poultry Breeding Engineering Technology Center of Shandong Province, Ji'nan, 250023, China. lei_qiuxia@163.com
Language :
English
Title :
Deciphering the miRNA transcriptome of breast muscle from the embryonic to post-hatching periods in chickens.
We thank International Science Editing (http://www.internationalscienceediting.com) for editing this manuscript.This research was funded by Natural Science Foundation of Shandong province (ZR2019BC077); Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs (poultrylab2019–3); Earmarked Fund for Modern Agro-industry Technology Research System (CARS-41); Jinan Layer Experiment Station of China Agriculture Research System (CARA-40-S12); Collection, Protection and Accurate Identification of Livestock Germplasm Resources (2019LZGC019); Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences (CXGC2016A04); Research and Demonstration on Key Technologies of Precision Breeding and Management of Laying Hens in Key R & D Projects in Shandong Province (2019JZZY020611); Shandong Provincial Key Laboratory of Special Construction Project (SDKL201810); and Construction of Subjects and Teams of Institute of Poultry Science (CXGC2018E11).
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