Changes in photosynthesis, growth and biomass composition in outdoor Chlorella g120 culture during the metabolic shift from heterotrophic to phototrophic cultivation regime

Masojídek, Jiří; Ranglová, Karolína; Rearte, Tomás Agustín; Celis Plá, Paula S.M.; Torzillo, Giuseppe; Benavides, Ana Margarita Silva; Neori, Amir; Gómez, Cintia; Álvarez-Gómez, Félix; Lukeš, Martin; Caporgno, Martín P.; Abdala, Roberto; Miazek, Krystian; Massocato, Thaís Fávero; da Silva, Jaqueline Carmo; Atzmüller, Richard; Al Mahrouqui, Hafidh; Estrella, Francisca Suárez; Figueroa, Felix L.

doi:10.1016/j.algal.2021.102303

Article (Scientific journals)

Changes in photosynthesis, growth and biomass composition in outdoor Chlorella g120 culture during the metabolic shift from heterotrophic to phototrophic cultivation regime

Masojídek, Jiří; Ranglová, Karolína; Rearte, Tomás Agustín et al.

2021 • In Algal Research, 56, p. 102303

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/295494

DOI
10.1016/j.algal.2021.102303

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Keywords :

Chlorophyll fluorescence; Microalgae; Photosynthesis; Physiology; Thin-layer cascade; Trophic conversion; Agronomy and Crop Science

Abstract :

[en] Up to now, scarce information has been available regarding major photobiochemical changes that accompany the metabolic shift from heterotrophy to phototrophy in microalgae cells. In the present trials the trophic conversion from heterotrophic to phototrophic growth regime was studied in the microalga Chlorella vulgaris g120 grown in outdoor thin-layer cascades. The crucial question was whether Chlorella g120 can undergo the photoacclimation of its photosynthetic apparatus under natural irradiance. As for comparison the phototrophic strain Chlorella vulgaris R-117 was cultured in parallel. Various in-situ and ex-situ monitoring techniques namely oxygen production and chlorophyll fluorescence were tested to characterize physiological response of the Chlorella g120 culture during photoacclimation and correlate it with growth. We show that Chlorella g120 can undergo the metabolic shift from heterotrophic to phototrophic growth regime, but its conversion is. A rather high respiration rate was interpreted as a strategy to dissipate the unused light energy. Chlorella g120 was found as the strain characterized by a small-antenna size strain (low chlorophyll/cell ratio < 1%) which should be favorable for light utilization in dense cultures. The high carotenoid/chlorophyll ratio (= 0.63) may be of interest from biotechnological point of view for carotenoid (namely lutein) production. The build-up of dissolved oxygen (DO) concentration and high electron transport rate measured in-situ indicated high photosynthetic activity of outdoor microalgae cultures. These variables are usable on-line as reliable markers as they reflect the physiological status of microalgae culture. Highly productive cultures, like Chlorella R-117 have high photosynthesis which is inevitably accompanied by the build-up on high DO gradients. To maintain high biomass production in such strains we have to find an interplay among photosynthetic activity, gradients of DO concentration, biomass density and culture layer thickness that can secure high growth rate (and productivity).

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Masojídek, Jiří; Laboratory of Algal Biotechnology, Centre ALGATECH, Institute of Microbiology, Czech Academy of Sciences, Třeboň, Czech Republic ; Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic

Ranglová, Karolína; Laboratory of Algal Biotechnology, Centre ALGATECH, Institute of Microbiology, Czech Academy of Sciences, Třeboň, Czech Republic ; Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic

Rearte, Tomás Agustín; Departamento de Recursos Naturales y Ambiente, Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, Argentina ; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Argentina

Celis Plá, Paula S.M.; Laboratory of Aquatic Environmental Research (LACER), Center of Advanced Studies, University of Playa Ancha, Viña del Mar, Chile ; HUB-AMBIENTAL UPLA, Vicerrectoría de Investigación Postgrado e Innovación, Universidad de Playa Ancha, Valparaíso, Chile

Torzillo, Giuseppe; CNR, Institute of Bioeconomy, Sesto Fiorentino, Italy ; Escuela de Biologia, CIMAR – Centro de Investigación en Ciencias del Mar y Limnología, Universidad de Costa Rica, San Pedro, Costa Rica

Benavides, Ana Margarita Silva; Escuela de Biologia, CIMAR – Centro de Investigación en Ciencias del Mar y Limnología, Universidad de Costa Rica, San Pedro, Costa Rica

Neori, Amir; Morris Kahn Marine Research Station, Marine Biology Department, The Leon H. Charney School of Marine Sciences, University of Haifa, Israel ; The Interuniversity Institute for Marine Sciences, Eilat, Israel

Gómez, Cintia; Department of Chemical Engineering, University of Almería, Almería, Spain

Álvarez-Gómez, Félix; University of Málaga, Institute of Blue Biotechnology and Development (IBYDA), Department of Ecology, Málaga, Spain

Lukeš, Martin; Laboratory of Algal Biotechnology, Centre ALGATECH, Institute of Microbiology, Czech Academy of Sciences, Třeboň, Czech Republic

More authors (9 more)

Language :

English

Title :

Changes in photosynthesis, growth and biomass composition in outdoor Chlorella g120 culture during the metabolic shift from heterotrophic to phototrophic cultivation regime

Publication date :

June 2021

Journal title :

Algal Research

ISSN :

2211-9264

Publisher :

Elsevier B.V.

Volume :

Pages :

102303

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://api.elsevier.com/content/article/PII:S2211926421001223?httpAccept=text/xml

Funders :

CAS Institute of Microbiology

Funding text :

This research was funded by the EU Program Horizon 2020 (project SABANA, grant no. 727874 ) and in part by National Sustainability Program of the Ministry of Education, Youth and Sports (project Algatech Plus LO1416 ) and cross-border InterReg Program between Czech Republic and Austria (Algenetics No. ATCZ15 ). Amir Neori was supported by BARD Research Grant Award No. US-4599-13R (The United States–Israel Binational Agricultural Research and Development Fund) and by the Israeli Ministry for Science and Technology and the Dead Sea-Arava Science Center (DSASC).

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