[en] Chondroitin sulfate (CS)-calcium complex (CSCa) was fabricated, and the structural characteristics of CSCa and its proliferative bioactivity to the chondrocyte were investigated in vitro. Results suggested calcium ions could bind CS chains forming polysaccharide-metal complex, and the maximum calcium holding capacity of CSCa reached 4.23 %. Characterization of CSCa was performed by EDS, AFM, FTIR, UV, XRD and 1H-NMR. It was found that calcium ions were integrated with CS by binding the sulfate or carboxyl groups. The thermal properties analysis indicated CSCa had a good thermal stability by TGA and DSC. CSCa could interact the calcium-sensing receptor increasing the intracellular calcium ions and influence the cell cycle. The TGF-β1 secretion induced by CSCa could activate the TGF-β/Smads pathway and change the genes associated proliferation expression ultimately leading to the chondrocyte proliferation. This research probably has an important implication for understanding the effect of CSCa on bone care as food supplements.
Zhang, Chunhui; Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Electronic address: dr_zch@163.com
Mo, Haizhen; School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China
Zhang, Hongru ; Université de Liège - ULiège > TERRA Research Centre
Qin, Xiaojie; Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China
Li, Juan; Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China
Zhang, Zhiqiang; Shandong Haiyu Biotechnology Co., Ltd., Jining, 272113, China
Richel, Aurore ; Université de Liège - ULiège > TERRA Research Centre > Smart Technologies for Food and Biobased Products (SMARTECH)
Language :
English
Title :
Fabrication of chondroitin sulfate calcium complex and its chondrocyte proliferation in vitro.
Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences
Funding text :
This work was supported by the grants of “ Central Public-interest Scientific Institution Basal Research Fund ( Y2020PT33 )” and “ Technology Innovation Program ( CAAS-ASTIP-2020-IFST-05 )”.
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