Anti-osteoporosis; Bone mineral density; Bone turnover markers; Chondroitin sulfate calcium complex; Gut microbiota; Chondroitin Sulfates; Calcium; Animals; Bacteria/metabolism; Bone Density/drug effects; Calcium/therapeutic use; Chondroitin Sulfates/therapeutic use; Dietary Supplements; Feces/microbiology; Femur/drug effects; Femur/pathology; Femur/ultrastructure; Gastrointestinal Microbiome/drug effects; Gastrointestinal Microbiome/physiology; Male; Metabolome/drug effects; Osteoporosis/diet therapy; Rats, Sprague-Dawley; Bone turnover marker; Calcium complexes; Chondroitin sulphate calcium complex; Chondroitinsulfate; In-vitro; Metabolite profiles; Ovariectomized rats; Organic Chemistry; Polymers and Plastics; Materials Chemistry
Abstract :
[en] Although chondroitin sulfate calcium complex (CSCa) was claimed to have the bioactivity for bone care in vitro, its anti-osteoporosis bioactivity was little reported in vivo. Here, the effects of CSCa on osteoporosis rats were investigated. Results showed that, compared with the osteoporosis rats, CSCa could improve the bone mineral density and microstructure of femur, and change the bone turnover markers level in serum. 16S rRNA sequencing and metabolomics analysis indicated CSCa intervention altered the composition of gut microbiota along with metabolite profiles in ovariectomized rat faeces. The correlation analysis showed some gut microbiota taxa were significantly correlated with osteoporosis phenotypes and the enriched metabolites. Taken together, dietary CSCa intervention has the potential to alleviate the osteoporosis and related symptoms probably involving gut microbiota or the metabolite profiles as demonstrated in rats. This study provides some scientific evidence for the potential effects of CSCa as the food supplement on the osteoporosis.
Zhang, Chunhui; Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: dr_zch@163.com
Qin, Xiaojie; Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Zhang, Hongru ; Université de Liège - ULiège > TERRA Research Centre
Zhang, Zhiqiang; Shandong Haiyu Biotechnology Co., Ltd., Jining 272113, China
Richel, Aurore ; Université de Liège - ULiège > TERRA Research Centre > Smart Technologies for Food and Biobased Products (SMARTECH)
Language :
English
Title :
Modulation of gut microbiota by chondroitin sulfate calcium complex during alleviation of osteoporosis in ovariectomized rats.
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