[en] Ceramides are central molecules in sphingolipid metabolism. They are involved in the regulation of cancer-cell growth, differentiation, senescence and apoptosis. To better understand how these secondary messengers induce their biological effects, adenocarcinoma cells (HCT116) were treated with exogenous long-chain ceramides (C16-ceramide) in order to mimic endogenous sphingolipids. This treatment induced a decrease of cell viability partly due to apoptosis as shown by PARP cleavage and a decrease of pro-caspase 3. Two-dimensional differential in-gel electrophoresis (2D-DIGE) revealed the differential expression of 51 proteins in response to C16-ceramide. These proteins are notably involved in cell proliferation, apoptosis, protein transport and transcriptional regulation. Among them, the cell death-promoting factor Btf was found to be implicated in the apoptotic signal triggered by ceramide. In adenocarcinoma cells, Btf regulates apoptosis related proteins such as Mdm2, p53, BAX and pBcl-2 and thus plays an important role in the ceramide mediated cell death. These findings bring new insight into the proapoptotic ceramide-dependent signaling pathway.
Centre/Unité de recherche :
Giga-Signal Transduction - ULiège
Disciplines :
Pharmacie, pharmacologie & toxicologie
Auteur, co-auteur :
Renert, Anne-Francoise
Leprince, Pierre ; Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Dieu, Marc
Renaut, Jenny
Raes, Martine
Bours, Vincent ; Centre Hospitalier Universitaire de Liège - CHU > Génétique
Chapelle, Jean-Paul ; Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
Piette, Jacques ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie - Immunologie - Département des sciences de la vie - GIGA-Research
Merville, Marie-Paule ; Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
Fillet, Marianne ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Langue du document :
Anglais
Titre :
The proapoptotic C16-ceramide-dependent pathway requires the death-promoting factor Btf in colon adenocarcinoma cells.
Date de publication/diffusion :
2009
Titre du périodique :
Journal of Proteome Research
ISSN :
1535-3893
eISSN :
1535-3907
Maison d'édition :
American Chemical Society (ACS), Washington, Etats-Unis - District de Columbia
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