[en] Aim: The Belgium’s strategy against COVID-19 was partly based on mass screening. Here, we reported the results observed in a Belgian mass screening center. Materials & methods: Between October 2020 and February 2021, 32,089 samples were collected analyzed with reverse-transcription PCR (Thermo Fisher Scientific kits and apparatus). Patients were categorized according to their contagiousness (extrapolated from the cycle threshold [Ct] values and the recommendation of Sciensano). Results: We observed association between Ct values and age, with higher Ct observed in extreme age groups (<6 years and >75 years). Conclusion: The analysis of the evolution of the contagiousness of these patients tested twice within a 7-day period showed the relevancy of the recommendation edited by Sciensano.
Disciplines :
Immunology & infectious disease
Author, co-author :
Dufour, Patrice ; Centre Hospitalier Universitaire de Liège - CHU > > Pool assistant - biologie clinique ; Department of Laboratory Medicine, Centre Hospitalier Régional de la Citadelle, Liège, 4000, Belgium
Paridaens, Henry; Department of Laboratory Medicine, Centre Hospitalier Régional de la Citadelle, Liège, 4000, Belgium
Senterre, Jean-Marc; Department of Laboratory Medicine, Centre Hospitalier Régional de la Citadelle, Liège, 4000, Belgium
Minon, Jean-Marc ; Department of Laboratory Medicine, Centre Hospitalier Régional de la Citadelle, Liège, 4000, Belgium
Language :
English
Title :
Relevance of cycle threshold values in mass screening by reverse-transcription-PCR during COVID-19 pandemic in Belgium: a decision-making support?
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Bibliography
European Centre for Disease Prevention and Control. COVID-19 situation update worldwide, as of week 22, updated 9 June 2022. https://www.ecdc.europa.eu/en/geographical-distribution-2019-ncov-cases
Han MS, Byun J-H, Cho Y, Rim JH. RT-PCR for SARS-CoV-2: quantitative versus qualitative. Lancet Infect. Dis. 21(2), 165 (2021).
Yu F, Yan L, Wang N et al. Quantitative detection and viral load analysis of SARS-CoV-2 in infected patients. Clin. Infect. Dis. 71(15), 793-798 (2020).
Singanayagam A, Patel M, Charlett A et al. Duration of infectiousness and correlation with RT-PCR cycle threshold values in cases of COVID-19, England, January to May 2020. Eurosurveillance 25(32), 2001483 (2020).
Bullard J, Dust K, Funk D et al. Predicting infectious severe acute respiratory syndrome coronavirus 2 from diagnostic samples. Clin. Infect. Dis. 71(10), 2663-2666 (2020).
He J, Guo Y, Mao R, Zhang J. Proportion of asymptomatic coronavirus disease 2019: a systematic review and meta-analysis. J. Med. Virol. 93(2), 820-830 (2021).
You Y, Yang X, Hung D, Yang Q, Wu T, Deng M. Asymptomatic COVID-19 infection: diagnosis, transmission, population characteristics. BMJ Support. Palliat. Care doi:10.1136/bmjspcare-2020-002813 (2021) (Epub ahead of print).
Ra SH, Lim JS, Kim G, Kim MJ, Jung J, Kim S-H. Upper respiratory viral load in asymptomatic individuals and mildly symptomatic patients with SARS-CoV-2 infection. Thorax 76(1), 61-63 (2021).
Yanes-Lane M, Winters N, Fregonese F et al. Proportion of asymptomatic infection among COVID-19 positive persons and their transmission potential: a systematic review and meta-analysis. PLOS ONE 15(11), e0241536 (2020).
Hanson KE, Caliendo AM, Arias CA et al. Infectious Diseases Society of America guidelines on the diagnosis of coronavirus disease 2019 (COVID-19): serologic testing. Clin. Infect. Dis. doi: 10.1093/cid/ciaa1343 (2020) (Epub ahead of print).
Yu X, Li N, Dong Y. Observation on China's strategies to prevent the resurgence of the COVID-19 epidemic. Risk Manag. Healthc. Policy 14, 2011-2019 (2021).
Jamal SA, Farooq MU, Bidari V. Challenges associated with using cycle threshold (Ct) value of reverse-transcription polymerase chain reaction (RT-PCR) as a criteria for infectiousness of coronavirus disease 2019 (COVID-19) patients in India. Infect. Control Hosp. Epidemiol. 1-2 DOI: 10.1017/ice.2021.357 (2021).
Dahdouh E, Lazaro-Perona F, Romero-Gomez MP, Mingorance J, Garcia-Rodriguez J. Ct values from SARS-CoV-2 diagnostic PCR assays should not be used as direct estimates of viral load. J. Infect. 82(3), 414-451 (2021).
Rabaan AA, Tirupathi R, Sule AA et al. Viral dynamics and real-time RT-PCR Ct values correlation with disease severity in COVID-19. Diagnostics (Basel) 11(6), 1091 (2021).
Gallo Marin B, Aghagoli G, Lavine K et al. Predictors of COVID-19 severity: a literature review. Rev. Med. Virol. 31(1), 1-10 (2021).
Ade C, PumJ, Abele I, Raggub L, Bockmuhl D, Zollner B. Analysis of cycle threshold values in SARS-CoV-2-PCR in a long-term study. J. Clin. Virol. 138, 104791 (2021).
Buchan BW, Hoff JS, Gmehlin CG et al. Distribution of SARS-CoV-2 PCR cycle threshold values provide practical insight into overall and target-specific sensitivity among symptomatic patients. Am. J. Clin. Pathol. 154(4), 479-485 (2020).
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