Reference : Vector-mediated delivery of bcl-2 prevents degeneration of auditory hair cells and ne...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Vector-mediated delivery of bcl-2 prevents degeneration of auditory hair cells and neurons after injury.
Staecker, Hinrich [> > > >]
Liu, Wei [> > > >]
Malgrange, Brigitte mailto [Université de Liège - ULiège > > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc. - Neurologie >]
Lefèbvre, Philippe mailto [Université de Liège - ULiège > Département des sciences cliniques > Oto-rhino-laryngologie et audiophonologie >]
Van De Water, Thomas R [> > > >]
Yes (verified by ORBi)
[en] Adenoviridae ; Animals ; Animals, Newborn ; Apoptosis/genetics ; Brain-Derived Neurotrophic Factor/pharmacology ; Cell Survival ; Cells, Cultured ; Cisplatin/toxicity ; Genes, bcl-2/physiology ; Genetic Vectors ; Hair Cells, Auditory/drug effects/pathology ; Membrane Potential, Mitochondrial/drug effects/genetics ; Neomycin/toxicity ; Nerve Degeneration/genetics ; Neurons/drug effects/pathology ; Oxidative Stress ; Rats ; Rats, Wistar ; Simplexvirus ; Spiral Ganglion/drug effects/pathology
[en] OBJECTIVE: To test the hypothesis that bcl-2 prevents oxidative stress-induced apoptosis of auditory sensory cells in explants of the organ of Corti and dissociated cell cultures of the spiral ganglion. METHODS: Organ of Corti explants and dissociated spiral ganglion cell cultures obtained from 3-day-old (P3) rats or adult spiral ganglion cell cultures from 28-day-old (P28) rats were transduced with vectors containing a human bcl-2 gene. Cultures were then exposed to neomycin, cisplatin or subjected to withdrawal of neurotrophin supplementation. Outcome measures included hair cell and neuron counts, mitochondrial membrane potential and a histological measure of apoptosis. RESULTS: Expression of bcl-2 in the organ of Corti explants and neuronal cell cultures provided a significant level of protection against cell death. Bcl-2 expression in the organ of Corti explants also protected mitochondria from loss of membrane potential and blocked an early step in the commitment of hair cells to apoptosis. CONCLUSION: Expression of bcl-2 in cochlear tissues protects sensory cells from a variety of insults that have been demonstrated to damage the inner ear.
Copyright © 2007 S. Karger AG, Basel.

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