[en] China is the largest potato producing country worldwide, with this crop representing the fourth largest staple food crop in China. However, the steady presence of Potato spindle tuber viroid (PSTVd) over the past five decades has a significant economic impact on potato production. To determine why PSTVd control measures have been ineffective in China, more than 1 000 seed potatoes collected between 2009 and 2014 were subjected to PSTVd detection at the Supervision and Testing Center for Virus-free Seed Potatoes Quality, Ministry of Agriculture, China. A high PSTVd infection rate (6.5%) was detected among these commercial seed potatoes. Some breeding lines of potato collected from 2012 to 2015 were also tested for PSTVd infection, revealing a high rate of PSTVd contamination in these potato propagation materials. Furthermore, comparison of the full-length sequences of 71 different Chinese PSTVd isolates revealed a total of 74 predominant PSTVd variants, which represented 42 different sequence variants of PSTVd. Comparative sequence analysis revealed 30 novel PSTVd sequence variants specific to China. Comprehensive phylogenetic analysis uncovered a close relationship between the Chinese PSTVd sequence variants and those isolated from Russia. It is worth noting that three intermediate strains and six mild strains were identified among these variants. These results have important implications for explaining the ineffective control of PSTVd in China and thus could serve as a basic reference for designing more effective measures to eliminate PSTVd from China in the future.
Disciplines :
Microbiology
Author, co-author :
Qiu, Cai-Ling; Northeast Agricultural University, Harbin, China ; Virus-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, China
Zhang, Zhi-Xiang; State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
Li, Shi-Fang; State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
Bai, Yan-Ju; Northeast Agricultural University, Harbin, China ; Virus-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, China
Liu, Shangwu ; Virus-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, China
Fan, Guo-Quan; Virus-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, China
Gao, Yan-Ling; Northeast Agricultural University, Harbin, China ; Virus-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, China
Zhang, Wei; Northeast Agricultural University, Harbin, China ; Virus-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, China
Zhang, Shu; Virus-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, China
Lü, Wen-He; Northeast Agricultural University, Harbin, China
Lü, Dian-Qiu; Virus-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, China
Language :
English
Title :
Occurrence and molecular characterization of Potato spindle tuber viroid (PSTVd) isolates from potato plants in North China
Using PSTree Vd-f seeds for o plantatpoting is commonly considered to be crucial, representing the most effective strategy for eliminating PSTVd from potato crops. Although virus (including viroid)-free seed potatoes have been produced and sold by a broad array of entities, including research institutes, universities, seed companies, private companies, and even small farmers (Jansky et al. 2009), PSTVd still exists in China and has not exhibited an obvious decline. This problem may be explained by the low quality of seed potatoes. T s p q the Supervision and T C irus-free Seed PoV - tatoes Quality was established at the Institute of Virus-free Seedling Research, Heilongjiang Academy of Agricultural Sciences under the support of the Ministry of Agriculture of China. One of the tasks of this Center is to test for PSTVdThe work was supported by the China Agriculture Research System (CARS-10-P14), the National Science Foundation for Post-doctoral Scientists of China 125),10491(201 the Heilongjiang Funds for Distinguished oung YS,istcientChina (JC 201018), the Harbin Application T Research and Development Projects, China AEA the Y S S Heilongjiang Province, China (QC2015026).
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