Expression patterns; Family genes; Gossypium arboreum; Cotton Fiber; Plant Science
Abstract :
[en] MAIN CONCLUSION: GL2-interacting-repressor (GIR) family members may contribute to fiber/fuzz formation via a newly discovered unique pathway in Gossypium arboreum. There are similarities between cotton fiber development and the formation of trichomes and root hairs. The GL2-interacting-repressors (GIRs) are crucial regulators of root hair and trichome formation. The GaFzl gene, annotated as GaGIR1, is negatively associated with trichome development and fuzz initiation. However, there is relatively little available information regarding the other GIR genes in cotton, especially regarding their effects on cotton fiber development. In this study, 21 GIR family genes were identified in the diploid cotton species Gossypium arboreum; these genes were divided into three groups. The GIR genes were characterized in terms of their phylogenetic relationships, structures, chromosomal distribution and evolutionary dynamics. These GIR genes were revealed to be unequally distributed on 12 chromosomes in the diploid cotton genome, with no GIR gene detected on Ga06. The cis-acting elements in the promoter regions were predicted to be responsive to light, phytohormones, defense activities and stress. The transcriptomic data and qRT-PCR results revealed that most GIR genes were not differentially expressed between the wild-type control and the fuzzless mutant line. Moreover, 14 of 21 family genes were expressed at high levels, indicating these genes may play important roles during fiber development and fuzz formation. Furthermore, Ga01G0231 was predominantly expressed in root samples, suggestive of a role in root hair formation rather than in fuzz initiation and development. The results of this study have enhanced our understanding of the GIR genes and their potential utility for improving cotton fiber through breeding.
Disciplines :
Agriculture & agronomy
Author, co-author :
Feng, Xiaoxu ; Université de Liège - ULiège > TERRA Research Centre ; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China
Cheng, Hailiang; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China
Zuo, Dongyun; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China
Zhang, Youping; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China
Wang, Qiaolian; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China
Lv, Limin; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China
Li, Shuyan; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China
Yu, John Z; Southern Plains Agricultural Research Center, USDA-ARS, Crop Germplasm Research Unit, 2881 F&B Road, College Station, Texas, 77845, USA. john.yu@usda.gov
Song, Guoli ; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China. songguoli@caas.cn
Language :
English
Title :
Genome-wide identification and expression analysis of GL2-interacting-repressor (GIR) genes during cotton fiber and fuzz development.
Publication date :
2022
Journal title :
Planta
ISSN :
0032-0935
eISSN :
1432-2048
Publisher :
Springer Science and Business Media Deutschland GmbH, Germany
NSCF - National Natural Science Foundation of China [CN] USDA - United States Department of Agriculture [US-DC] [US-DC]
Funding text :
This work was funded by grants from the National Natural Science Foundation of China (No. 31621005), the National Natural Science Foundation of China (No. 31901581), the National Key R & D Plan of China (No. 2018YFD0100402) and the United States Department of Agriculture—Agricultural Research Service (USDA-ARS Project No. 3091-21000044-00D). The funders had no role in the experimental design, data collection and analysis, or in writing work.The authors thank Lima Soares Emanoella from Plant Genetics Laboratory of Gembloux Agro Bio-Tech, University of Liège for kindly revising the draft.
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