2D-DIGE; Parathyroid carcinoma; biomarkers; case report; parathyroid adenoma; proteomics; Adenoma/diagnosis; Adenoma/pathology; Humans; Male; Middle Aged; Parathyroid Neoplasms/diagnosis; Parathyroid Neoplasms/pathology; Proteomics/methods; Adenoma; Parathyroid Neoplasms; Biochemistry; Molecular Biology; Genetics; Cancer Research
Abstract :
[en] BACKGROUND/AIM: The lack of specific parathyroid carcinoma (PC) biomarkers in clinical practice points out the importance of analyzing the proteomic signature of this cancer. We performed a comparative proteomic analysis of PC and parathyroid adenoma (PA) co-existing in the same patient.
PATIENTS AND METHODS: PC and PA were taken from a 63-year-old patient. Using two-dimensional differential gel electrophoresis (2D-DIGE) coupled to mass spectrometry we examined the differences between PC and PA proteins. For validation, additional PC and PA samples were obtained from 10 patients. Western blot analysis was used to validate the difference of expression observed with 2D-DIGE analysis. Bioinfomatic analysis was performed using QIAGEN's Ingenuity Pathways Analysis (IPA) to determine the predominant canonical pathways and interaction networks involved.
RESULTS: Thirty-three differentially expressed proteins were identified in PC compared to PA. Among these, ubiquitin C-terminal hydrolase-L1 (UCH-L1) was highly overexpressed in PC. The result was confirmed by Western Blot analysis in additional PC samples.
CONCLUSION: Our comparative proteomic analysis of co-existing neoplasms allowed detecting specific and peculiar differences between PC and PA overcoming population biological variability.
Disciplines :
Oncology
Author, co-author :
Ciregia, Federica ; Université de Liège - ULiège > GIGA > GIGA I3 - Rheumatology ; Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy
Cetani, Filomena; Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy
Pardi, Elena; Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy
Soggiu, Alessio; Surgical and Dental Sciences-One Health Unit, Department of Biomedical, University of Milano, Milan, Italy
Piras, Cristian; Department of Health Sciences, Campus Universitario "S. Venuta", University "Magna Græcia" of Catanzaro, Catanzaro, Italy
Zallocco, Lorenzo; Department of Pharmacy, University of Pisa, Pisa, Italy
Borsari, Simona; Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy
Ronci, Maurizio; Department of Pharmacy, University G. d'Annunzio of Chieti-Pescara, Chieti, Italy
Caruso, Vanni; School of Pharmacy & Pharmacology - College of Health and Medicine, University of Tasmania, Hobart, TAS, Australia
Marcocci, Claudio; Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy
Mazzoni, Maria Rosa; Department of Pharmacy, University of Pisa, Pisa, Italy
Lucacchini, Antonio; Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy
Giusti, Laura; School of Pharmacy, University of Camerino, Camerino, Italy laura.giusti@unicam.it
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