[en] Sweet cherry (Prunus avium L.) is a stone fruit widely consumed and appreciated for its organoleptic properties, as well as its nutraceutical potential. We here investigated the characteristics of six non-commercial Tuscan varieties of sweet cherry maintained at the Regional Germplasm Bank of the CNR-IBE in Follonica (Italy) and sampled ca. 60 days post-anthesis over three consecutive years (2016-2017-2018). We adopted an approach merging genotyping and targeted gene expression profiling with metabolomics. To complement the data, a study of the soluble proteomes was also performed on two varieties showing the highest content of flavonoids. Metabolomics identified the presence of flavanols and proanthocyanidins in highest abundance in the varieties Morellona and Crognola, while gene expression revealed that some differences were present in genes involved in the phenylpropanoid pathway during the 3 years and among the varieties. Finally, proteomics on Morellona and Crognola showed variations in proteins involved in stress response, primary metabolism and cell wall expansion. To the best of our knowledge, this is the first multi-pronged study focused on Tuscan sweet cherry varieties providing insights into the differential abundance of genes, proteins and metabolites.
Berni, Roberto ; Université de Liège - ULiège > TERRA Research Centre > Echanges Eau - Sol - Plantes ; Department of Life Sciences, University of Siena, via P.A. Mattioli 4, I-53100, Siena, Italy
Charton, Sophie ; Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 41, Rue du Brill, L-4422, Belvaux, Luxembourg
Planchon, Sébastien ; Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 41, Rue du Brill, L-4422, Belvaux, Luxembourg
Legay, Sylvain; Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 5, rue Bommel, L-4940, Hautcharage, Luxembourg
Romi, Marco; Department of Life Sciences, University of Siena, via P.A. Mattioli 4, I-53100, Siena, Italy
Cantini, Claudio; Istituto per la BioEconomia (IBE CNR), Dipartimento di Scienze BioAgroAlimentari, via Aurelia 49, 58022, Follonica, Italy
Cai, Giampiero; Department of Life Sciences, University of Siena, via P.A. Mattioli 4, I-53100, Siena, Italy
Hausman, Jean-Francois; Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 5, rue Bommel, L-4940, Hautcharage, Luxembourg
Renaut, Jenny; Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 41, Rue du Brill, L-4422, Belvaux, Luxembourg. jenny.renaut@list.lu
Guerriero, Gea ; Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 5, rue Bommel, L-4940, Hautcharage, Luxembourg. gea.guerriero@list.lu
Language :
English
Title :
Molecular investigation of Tuscan sweet cherries sampled over three years: gene expression analysis coupled to metabolomics and proteomics.
During the acquisition of the data presented in this study, R.B. was in receipt of the PhD fellowship “Pegaso” financed by the Region Tuscany. Aude Corvisy and Laurent Solinhac are gratefully acknowledged for technical help in sequencing and genotyping.
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